Enbucrilate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

other: Analogy

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study and GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2014

Report date:

2014

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Butyl cyanoacetate

EC Number:

226-730-1

EC Name:

Butyl cyanoacetate

Cas Number:

5459-58-5

Molecular formula:

C7H11NO2

IUPAC Name:

butyl cyanoacetate

Test material form:

liquid

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Duration of test (contact time):

28 d

Results and discussion

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

82

Sampling time:

28 d

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

Despite the fact that N-Butyl- Cyano-Acrylate will immediately polymerize in the presence of water, the biodegradation behaviour of the monomer was read across to the biodegradability of N-Butyl- Cyano-Acetate. The rationale is based on structural similarity of the molecules and providing evidence for the circumstances where monomers may occur at very low concentrations of N-Butyl- Cyano-Acrylate.

The mean biodegradation level of 10% of N-Butyl-
Cyano-Acetate was reached at day 2 (ThODNH4, ThODNO3).
At the end of the 10-day window at day 12, the
degradation of N-Butyl-Cyano-Acetate was 88%
(ThODNH4) and 71% (ThODNO3) and therefore the 10 day
window criterion was passed. The mean biodegradation
at test end after 28 days was 103% (ThODNH4) and 82%
(ThODNO3).

Therefore, N-Butyl-Cyano-Acetate is considered to be readily
biodegradable based on ThODNH4 and ThODNO3.

Executive summary:

Providing evidence for circumstances where monomers of N-Butyl- Cyano-Acrylate may not instantly polymerize in water but may occur at very low concentrations a read across to the biodegradability of N-Butyl- Cyano-Acetate was done based on structural similarity. The test item N-Butyl-Cyano-Acetate was investigated for its ready biodegradability in a manometric respirometry test (OECD 301F) over a period of 28 days.

The test item N-Butyl-Cyano-Acetate contains nitrogen; therefore the evaluation of biodegradation has to be based ThODNH4 and ThODNO3. The criterion for ready biodegradability under the conditions of a manometric respirometry test is the 10-day window, describing the period between reaching at least 10% degradation and 60% degradation. This period should not exceed 10 days.

The mean biodegradation level of 10% of N-Butyl- Cyano-Acetate was reached at day 2 (ThODNH4, ThODNO3). At the end of the 10-day window at day 12, the degradation of N-Butyl-Cyano-Acetate was 88% (ThODNH4) and 71% (ThODNO3) and therefore the 10 day window criterion was passed. The mean biodegradation at test end after 28 days was 103% (ThODNH4) and 82% (ThODNO3). Therefore, N-Butyl-Cyano-Acetate is considered to be readily biodegradable based on ThODNH4 and ThODNO3.