Registration Dossier
Registration Dossier
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EC number: 429-990-6 | CAS number: -
Endpoint summary
Administrative data
Description of key information
Additional information
Biodegradation in water
Two studies were conducted to assess the biodegradability of the test item Incozol LV. In the CO2 evolution test according to OECD TG 301B and in the closed bottle test according to OECD TG 301 D the test item do not reach the threshold of degradation to fulfil the stringent criteria of OECD TG for readily biodegradation. According to ECHA Guidance IR/CSR chapter R.7B ‘Biodegradation above 20% of theoretical (measured as BOD, DOC removal or COD) may be regarded as evidence of inherent, primary biodegradability’. In the closed bottle test Incozol LV attained 26 % degradation after 28 days and in the CO2 evolution test 47 % degradation. Thus, Incozol LV is regarded as inherently biodegradable.
CO2 evolution test
A study was conducted according to OECD TG 301B and Directive 92/69/EEC Part C4 to assess the ready biodegradability of Incozol LV by measuring carbon dioxide evolution. Therefore, weights of 0.0756, 0.0756 and 0.0763 g Incozol LV product were added, corresponding to between 25.2 and 25.4 mg/L, and were intended to give concentrations of Incozol LV equating to 15 mg organic carbon per litre. A nominal concentration of sodium benzoate corresponding to 15 mg C/L was used as reference. Rapid CO2 generation began immediately and continued until Day 8. The final CO2 yields for the test substance, expressed as a percentage of theoretical, were 48 % in one replicate and 46 % in the other. These data are above the 10 % level generally regarded as representing the threshold of significant degradation, but below the 60 % 'pass' level that represents complete mineralisation. According to ECHA Guidance IR/CSR chapter R.7B ‘Biodegradation above 20 % of theoretical (measured as BOD, DOC removal or COD) may be regarded as evidence of inherent, primary biodegradability’, thus, Incozol LV is regarded as inherent primarily biodegradable. All the validity criteria were met and the results of this study may, therefore, be considered entirely valid.
Closed bottle test
A study was conducted according to OECD TG 301D to investigate the ready biodegradability of the test material when exposed to sewage treatment microorganisms under aerobic conditions. The oxygen depletion of the inoculated control did not exceed 1.5 mg O2/L after 28 days, the residual oxygen concentration in the test bottles remained at 5.20 mg O2/L or greater in all test vessels and the difference between the extremes of replicate oxygen depletion values at the end of the test was less than 20 % in all vessels thereby satisfying the validation criteria. Nitrate and nitrite analysis of the test solutions at each sampling occasion confirmed that no significant oxygen consumption as a result of nitrification occurred during the study. The test material attained 26 % degradation after 28 days and, therefore, cannot be considered as readily biodegradable under the strict terms and conditions of OECD TG 301D. According to ECHA Guidance IR/CSR chapter R.7B ‘Biodegradation above 20 % of theoretical (measured as BOD, DOC removal or COD) may be regarded as evidence of inherent, primary biodegradability’, thus, Incozol LV is regarded as inherently biodegradable.
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