Alkanes, C10-13, chloro

Long-term toxicity to fish

Administrative data

Endpoint:

adult fish: sub(lethal) effects

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Limited reporting in expert review; cited in final RAR (EU, 2000); considered acceptable for assessment.

Data source

Reference

Reference 1

Reference Type:

review article or handbook

Title:

Unnamed

Year:

1975

Reference 2

Reference Type:

secondary source

Title:

Unnamed

Year:

2000

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)

Deviations:

yes

Remarks:

incomplete reporting (no data on concentrations tested, no detailed results presented, only mininmal description of test methods)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Alkanes, C10-13, chloro

EC Number:

287-476-5

EC Name:

Alkanes, C10-13, chloro

Cas Number:

85535-84-8

IUPAC Name:

Alkanes, C10-C13, Chloro

Details on test material:

- Name of test material (as cited in study report): C10-12 chlorinated paraffin
- Substance type: technical product
- Physical state: no data
- Analytical purity: no data
- Impurities (identity and concentrations): no data
- Composition of test material, percentage of components: C10-12 Chlorinated paraffin (58% chlorinated)

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
none

Sampling and analysis

Analytical monitoring:

not specified

Details on sampling:

no data

Test solutions

Vehicle:

not specified

Details on test solutions:

no data

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow trout

Study design

Test type:

flow-through

Water media type:

not specified

Limit test:

no

Total exposure duration:

20 d

Post exposure observation period:

no data

Test conditions

Hardness:

no data

Test temperature:

10oC

pH:

no data

Dissolved oxygen:

no data

Salinity:

no data

Nominal and measured concentrations:

no data

Details on test conditions:

TEST CONCENTRATIONS
- Test concentrations: not clear from report, but appears to range between 0.04 and 100 mg/L

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Duration:

20 d

Dose descriptor:

NOEC

Effect conc.:

< 0.04 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

behaviour

Details on results:

Progressive loss of motor function leading to immobility within 15-20 days

Results with reference substance (positive control):

no data

Reported statistics and error estimates:

none

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

Exposure of rainbow trout (Onchorynchus mykiss) to a C10-12 chlorinated paraffin (58% chlorinated) at concentrations down to 0.04 mg/L resulted in progressive loss of motor function, and in some cases, death. The NOEC for this material was less than 0.04 mg/L. A LC50 of more than 100 mg/L after 24 days was noted.

Executive summary:

According to an expert review, Rainbow trout (Onchorynchus mykiss) were exposed to a C10-12 chlorinated paraffin (58% chlorinated) in a flow-through system at 10^oC for up to 20 days. Although not clear from the report, it appears concentrations of between 0.04 and 100 mg/L were used. A LC50 of more than 100 mg/L after 24 days was noted. Various sub-lethal effects were reported resulting in a progressive loss of motor function and immobilisation, beginning within 15 to 20 days of the start of exposure. When death occured it resulted from debilitation, cessation of feeding and other secondary effects. The NOEC for the C10-12 chlorinated paraffin was less than 0.04 mg/L in this study.