3,7,11,15-tetramethylhexadec-1-en-3-ol

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study well documented, meets generally accepted scientific principles, acceptable for assessment.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1970

Report date:

1970

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

BASF-Test, no guideline available at that time.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

3,7,11,15-tetramethylhexadec-1-en-3-ol

EC Number:

208-008-8

EC Name:

3,7,11,15-tetramethylhexadec-1-en-3-ol

Cas Number:

505-32-8

Molecular formula:

C20H40O

IUPAC Name:

3,7,11,15-tetramethylhexadec-1-en-3-ol

Details on test material:

- Name of test material (as cited in study report): Isophytol G
- Physical state: fluid
- Analytical purity: ca. 90 %
- Impurities (identity and concentrations): not reported

Test animals / tissue source

Species:

rabbit

Strain:

Vienna White

Details on test animals or tissues and environmental conditions:

TEST ANIMALS
- Source: no data
- Age at study initiation: no data
- Weight at study initiation: 2.01 and 1.92 kg

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: NaCl-treated eye of the same animals served as control

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 50 µl
- Concentration (if solution): undiluted

Duration of treatment / exposure:

not removed

Observation period (in vivo):

3 days (free of symptoms)

Number of animals or in vitro replicates:

2 males

Details on study design:

REMOVAL OF TEST SUBSTANCE
- Washing (if done): not washed out

TOOL USED TO ASSESS SCORE: fluorescein (72h after test substance administration)

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

cornea opacity score

Basis:

animal #1

Time point:

other: 24, 48, 72 h

Score:

0.33

Max. score:

4

Reversibility:

fully reversible within: 2 days

Results 2

Irritation parameter:

cornea opacity score

Basis:

animal #2

Time point:

other: 24, 48, 72 h

Score:

0.67

Max. score:

4

Reversibility:

fully reversible within: 3 days

Results 3

Irritation parameter:

conjunctivae score

Basis:

animal #1

Time point:

other: 24, 48, 72 h

Score:

0.33

Max. score:

3

Reversibility:

fully reversible within: 2 days

Results 4

Irritation parameter:

conjunctivae score

Basis:

animal #2

Time point:

other: 24, 48, 72 h

Score:

0.67

Max. score:

3

Reversibility:

fully reversible within: 3 days

Any other information on results incl. tables

One hour after application slight erythema of the conjunctiva (some vessels definitely injected; Draize 1) was found, 24 hours after application slight erythema of the conjunctiva (Draize 1) and slight corneal opacity (Draize 1) were observed, but 2 and 3 days after application, respectively, the symptoms had subsided, and observation was stopped. No effects on the iris or chemosis were reported in this study.

NaCl-treated control eyes were without symptoms during the whole obsevation period. Reported findings in the investigation were converted to Draize scores according to Grundler (1983).

Findings at different timepoints after test substance application, converted to Draize scores:

	1 h		24 h		48 h		72 h
Irritation parameter	#1	#2	#1	#2	#1	#2	#1	#2
Cornea	0	0	1	1	0	1	0	0
Conjunctiva erythema	1	1	1	1	0	1	0	0

# animal number