Potassium dimethyldithiocarbamate

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

1980

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions (E.coli strain is missing)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1980

Report date:

1980

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

yes

Remarks:

Only four strains were used although OECD 471 orders five. 2-Aminoanthracene was the only positive control for the activation assays, which is not recommended by OECD 471.

GLP compliance:

no

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Sodium dimethyldithiocarbamate

EC Number:

204-876-7

EC Name:

Sodium dimethyldithiocarbamate

Cas Number:

128-04-1

IUPAC Name:

sodium dimethyldithiocarbamate

Details on test material:

- Name of test material (as cited in study report): Wingstop B
- Lot/batch No.: TT 6-502, 385481
- Purity: no data
- Other: Wingstop B is a rubber chemical used to retard vulcanization.

Method

Target gene:

His auxotrophy, defective DNA repair activity (uvrB) and defective LPS barrier (rfa)

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Additional strain / cell type characteristics:

other: Ampicillin resistance (TA 98 and TA 100 only)

Metabolic activation:

with and without

Metabolic activation system:

S9 mix from livers of rats treated with Aroclor 1254

Test concentrations with justification for top dose:

–S9: 32, 63, 320 and 630 µg/plate (TA 100 and 1535 additionally: 10, 32, 100 and 320 µg/plate)
+S9: 10, 32, 100, 320 and 1000 µg/plate

Vehicle / solvent:

DMSO

Controls

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

Remarks:

DMSO

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: 2-Nitrofluorene (2NF, -S9), Quinacrine mustard (QM, -S9), Sodium azide (NaN3, -S9); strain not reported All strains: 2-aminoanthracene (2-AA, +S9)

Details on test system and experimental conditions:

METHOD OF APPLICATION: in agar (plate incorporation)

Results and discussion

Test results

Species / strain:

S. typhimurium TA 100

Metabolic activation:

without

Genotoxicity:

positive

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

valid

Untreated negative controls validity:

not applicable

Positive controls validity:

valid

Any other information on results incl. tables

Table 7.6.1 -A1 Gene mutation assay (plate incorporation) Average revertants per plate in tester strains TA98, TA 100, TA 1535 and TA 1537 without S9-mix Concentration [µg/plate] Revertants/plate [mean of 3 plates] TA 98 TA 100 TA 1535 TA 1537 Solvent control 47 63 14 8 32 57 140 20 17 63 45 204 37 21 320 51 234 59 22 630 47 185 41 21 Pos. control 1168 1191 875 1988 Solvent control – 64 8 – 10 – – 8 – 32 – 84 8 – 100 – 161 18 – 320 – 200 34 – Pos. control – 1056 931 – Table 7.6.1-A2 Gene mutation assay (plate incorporation) Average revertants per plate in tester strains TA98, TA 100, TA 1535 and TA 1537 with S9-mix (rat)
Concentration [µg/plate]		Revertants/plate [mean of 3 plates]
		TA 98		TA 100		TA 1535	TA 1537
Solvent control		21		76		6	6
10		22		150		11	5
32		37		136		18	10
100		34		247		25	12
320		26		75		14	6
1000		23		–		5	5
Pos. control	1169		1034		128		128