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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil macroorganisms except arthropods
Administrative data
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2018-08-10 to 2019-04-17
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test substrate
- Vehicle:
- no
- Details on preparation and application of test substrate:
- The artificial soil was prepared as follows:
a) 10 per cent sphagnum peat (pH 5.62, the water content measured before use was 1.78%)
b) 20 per cent kaolin clay (kaolinite content was greater than 30%);
c) 70 per cent industrial sand (fine sand should be dominant with more than 50 per cent of the particles between 50 and 200 microns);
According to above proportions, prepare 6 groups of 2500 g artificial soil for he test (50 g industrial sand was deducted from amount of industrial sand to use for mixing with test item). The dry constituents are blended in the correct proportions and mixed thoroughly in blender. The moisture content was adjusted to about 20% of the dry weight with distilled water (500 ml). After test item application, the moisture content was adjusted to about 30-35% (the mixture should be moist but not so wet that water appears when the artificial soil is compressed). pH of artificial soil was determined before the test.
0.2507, 0.4520, 0.8020, 1.4056, 2.5060 g of test item were weighed and mixed with 50 g industrial sand respectively. The treated sand was mixed thoroughly with proper artificial soil (dry weight was 2450 g), and distilled water (250 ml) was added to adjust the moisture content to about 30% of dry weight and mixed to be homogeneous. For each treated soil, four 750 g of the wet test medium were weighed and put into four labelled test containers with glass plate.
A concurrent control group with four replicates maintained under identical conditions only without test item application was performed in parallel. 50 g industrial sand was applied to properly pre-moistened artificial soil ( dry weight was 2450 g), then distilled water (250 ml) was added to adjust the moisture content to about 30% of dry weight and mixed to be homogeneous.
Test organisms
- Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- The test earthworm (Eisenia foetida) was obtained from Chenggong Earthworm Raising Farm in Dongli District, Tianjin. The domestication conditions of earthworms were 20±2° C, 80±15% relative humidity and continuous light.
The adult (at least two months old with clitellum) worms with an individual weight of 0.3023~0.5604 g were used in the test.
Study design
- Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- no
- Total exposure duration:
- 14 d
Test conditions
- Test temperature:
- 18,7-20,3 °C
- pH:
- 5.51-5.91
- Moisture:
- 29.8-29.9%
- Details on test conditions:
- Test concentrations included I 00, 180, 320, 560 and 1000 mg/kg dry soil weight. A blank control group was conducted in parallel. Four replicates were used for the test groups and control group.
For each test container, 10 earthworms (which had been conditioned in an artificial soil, washed, dried on filter paper and weighed before use) were placed on the surface of test medium. The containers were covered with a glass plate to prevent the earthworms from escaping and the test medium from drying. The containers were kept under the test conditions for 14 days.
he mortality was assessed by emptying test medium onto a plate, sorting worms from the medium and testing their reaction to a mechanical stimulus at the front end at the 7th and 14th day. Any behavioural signs was recorded. After the 7-day assessment worms and medium was replaced in the test containers.
At the start and the end of the test, the individual weights of all live worms in each group were measured and recorded. The worms were washed prior to weighing (with distilled water) and the excess water was removed by placing the wonns briefly on filter paper.
At the beginning and the end of the test, soil moisture content was determined at the test groups and control medium. 1 replicate at the start and 4 replicates at the end. The moisture content was determined by drying a small sample at 105°C for 3 hours and re-weighing.
At the beginning of the test, soil pH was measured. I replicate was measured in test group and control group. The method is as followed: wet soil was dried at room temperature for at least 21 h. A suspension of the soil ( containing 5 grams of soil) was then made up in five times its volume of a 0.01 M solution of analytical grade calcium chloride (CaCl2). The suspension was then shaken thoroughly for six minutes and then left to settle for at 2 hours. The pH of the liquid phase is then measured using a pH-meter. - Nominal and measured concentrations:
- 180, 320, 560 and 1000 mg/kg dry soil weight
- Reference substance (positive control):
- yes
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- 207 mg/kg soil dw
- Basis for effect:
- mortality
- Duration:
- 7 d
- Dose descriptor:
- LC50
- Effect conc.:
- 341.6 mg/kg soil dw
- Basis for effect:
- mortality
- Details on results:
- During the exposure period, there was no mortality or abnormal signs observed at control group and test group of 100 mg/kg dry soil weight; At test groups of 180, 320, 560 and 1000 mg/kg dry soil weight, there were flexible body, loose and disconnect canker observed, and earthworms even dead, and the number of deaths increased with increasing concentrations, all earthworms dead in 7th day of 1000 mg/kg dry soil weight test group.
- Results with reference substance (positive control):
- The 14-day LC50 of the reference substance to earthworm is 21.1 mg a.i./kg dry soil weight, with 95% confidence limits of 17.7-25.1 mg a.i./kg dry soil weight, which met the requirement of 20-80 mg a.i./kg dry soil weight in the guideline.
- Reported statistics and error estimates:
- Trimmed spearman-karber method (TSK,Version 1.5) was used to evaluate the data, LC50 and 95% confidence limit.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- According to the results, the acute toxicity of Sa 57 to the earthworm (Eisenia foetida) was:
LC50 ( 7 d): 341.6 mg/kg dry soil weight > 95% confidence limit were 300.1-388.8 mg/kg dry soil weight;
LC50 (14 d): 207.0 mg/kg dry soil weight >95% confidence limit were 184.0-232.8 mg/kg dry soil weight - Executive summary:
This study was performed to assess the acute toxicity of Sa 57 to the earthworm (Eiseniajoetida).
Based on the results of the preliminary test, the definitive study was conducted at concentrations of 100, 180, 320, 560 and 1000 mg/kg dry soil weight. A concurrent control group was conducted in parallel. For the test and control group, 4 replicates were used with 10 worms in each replicate.
Artificial soil was used in this study. The test duration was 14 days, assessment of mortality and abnormal signs was conducted at 7 and 14 day of test.
During the exposure period, there was no mortality or abnormal signs observed at control group and test group of 100 mg/kg dry soil weight; At test groups of 180, 320, 560 and 1000 mg/kg dry soil weight, there were flexible body, loose and disconnect canker observed, and earthworms even dead, and the number of deaths increased with increasing concentrations, all earthworms dead in 7th day of 1000 mg/kg dry soil weight test group.According to the results, the acute toxicity of Sa 57 to the earthworm (Eisenia foetida) was:
LC50 ( 7 d): 341.6 mg/kg dry soil weight > 95% confidence limit were 300.1-388.8 mg/kg dry soil weight;
LC50 (14 d): 207.0 mg/kg dry soil weight >95% confidence limit were 184.0-232.8 mg/kg dry soil weight
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