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EC number: 602-927-1 | CAS number: 123312-89-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 29 Jul 1992
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- other: 87/302/EEC (page 118-122): Acitivated sludge respiration inhibition test
- Deviations:
- yes
- Remarks:
- see 'Any other information on materials and methods incl. tables'
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- - Preparation: 20.27, 8.23, 3.20, 1.33, 0.53 and 0.26 mg of the test substance were weighed and added to the test medium. The volume was adjusted to 200 mL with water and aerated for 3 hours.
- Test organisms (species):
- activated sludge
- Details on inoculum:
- - Source: Activated sludge was collected from the sewage treatment plant.
- pH after collection: 7.1
- Preparation of inoculum for exposure - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20 ± 2 °C
- Nominal and measured concentrations:
- - Nominal concentrations: 0 (control), 1.3, 2.7, 6.7, 16.0, 41.2 and 101.4 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL BOD flasks with gas inlet
- No. of vessels per concentration: 1
- No. of vessels per control: 2
- No. of vessels per reference control: 1 per concentration
- Sludge concentration: 1.59 g/L (dry weight)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated drinking water
- Nutrient solution: 16g peptone, 11g meat extract, 3.0 g urea, 0.7 g NaCl, 0.4 g CaCl2.7H2O, 0.2 g MgSO4.7H2O and 2.8 g K2HPO4 dissolved and made up to one liter with dechlorinated drinking water
EFFECT PARAMETERS MEASURED: oxygen consumption
Oxygen consumption per hour was measured in mg/L with an ORION - Electrode Type 97-08, ORION Microprocessor Ionalizer 901 and plotted on a recorder. The inhibitions were calculated on the basis of the measured time-depent oxygen consumption of a blank and test solution. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol (3.2, 10.0 and 32.1 mg/L)
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks on result:
- other: NOEC value not presented in the study report; derived by the registrant
- Details on results:
- No inhibition in oxygen consumption was observed in all test substance treatments compared to the blank control.
- Results with reference substance (positive control):
- The 3-h EC50 of the reference substance was determined to be 13.3 mg/L.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The 3-h EC50 value is >100 mg/L for microorganisms present in activated sludge from a sewage treatment plant. The NOEC is determined to be 100 mg/L.
- Executive summary:
The toxicity of the substance to microorganisms was investigated in an activated sludge respiration inhibition study according to 87/302/EEC (page 118-122) and in compliance with GLP criteria. In this study non-adapted activated sludge from a sewage was exposed to test substance concentrations of 0 (control), 1.3, 2.7, 6.7, 16.0, 41.2 and 101.4 mg/L. Nominal test concentrations were not analytically verified. The effects on microorganisms was determined by measuring total respiration rates and comparing these to an unexposed control. No inhibition in oxygen consumption was observed in all test substance treatments compared to the blank control. Based on these findings, a 3-h EC50 value of >100 mg/L was derived. Although no NOEC was derived in the study report, the absence of inhibition of respiration in the highest tested concentration of 100 mg/L justifies the NOEC to be determined at 100 mg/L.
Reference
Table: Results
Sample |
Conc. [mg/L] |
Consumption rate [mg/L/h] |
Inhibition [%] |
pH |
Blank 1 |
0.0 |
58.1 |
-3 |
8.1 |
Blank 2 |
0.0 |
54.4 |
3 |
8.1 |
Reference 1 |
32.1 |
12.8 |
77 |
8.2 |
Reference 2 |
10.0 |
31.9 |
43 |
8.2 |
Reference 3 |
3.2 |
54.6 |
3 |
8.1 |
Test conc. 1 |
101.4 |
61.2 |
-9 |
8.0 |
Test conc. 2 |
41.2 |
57.7 |
-3 |
8.0 |
Test conc. 3 |
16.0 |
58.9 |
-5 |
8.0 |
Test conc. 4 |
6.7 |
57.9 |
-3 |
8.0 |
Test conc. 5 |
2.7 |
58.8 |
-4 |
8.0 |
Test conc. 6 |
1.3 |
60.0 |
-7 |
8.0 |
Description of key information
The 3-h EC50 and NOEC are >100 and 100 mg/L, respectively, in microorganisms in STP sludge, 87/302/EEC, Baumann 1992
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 100 mg/L
Additional information
Table: Overview of available data on the toxicity to microorganisms
Species |
Guideline / GLP |
Endpoint |
Effect value |
Comment |
Reference |
Activated sludge from STP |
87/302/EEC / GLP |
3-h EC50 3-h EC20 3-h NOEC |
> 100 mg/L > 100 mg/L 100 mg/L* |
No inhibition in oxygen consumption was observed in all treatments |
Baumann, 1992 |
*: highest tested concentration
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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