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Diss Factsheets
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EC number: 480-060-6 | CAS number: 24065-33-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 8 Jul - 10 Aug 2005
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: secondary effluent of the domestic sewage treatment plant Wupper area water authority
- Preparation of inoculum for exposure: effluent concentration in reaction mixture was 5 mL/L
- Pretreatment: separation of coarse particles by filtration, aeration of mineral medium plus inoculum (filtrate) for 5 days
- Water filtered: yes - Duration of test (contact time):
- 28 d
- Initial conc.:
- 5.9 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: mineral medium
- Test temperature: 20 °C +/- 2 °C
- Aeration of dilution water: Aeriation for 5 days
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: closed bottles
- Number of culture flasks/concentration: 10
- Method used to create aerobic conditions: aeriation of the solution
SAMPLING
- Sampling frequency: 7,14, 21, 28 days
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, 10 replicates
- Toxicity control: yes, 10 replicates (test substance + sodium benzoate)
- Procedure control: yes, 10 replicates (sodium benzoate) - Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 2
- Sampling time:
- 28 d
- Details on results:
- The toxicity control showed 29% and 32% degradation after 14 days and 28 days, respectively. The used concentration of the test substance did not show toxic effects to bacteria.
- Results with reference substance:
- The reference substance (sodium benzoate) showed 73% and 86% degradation after 14 days and 28 days, respectively.
- Validity criteria fulfilled:
- yes
- Remarks:
- See 'Any other information on results incl. tables'.
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The test substance showed 2% degradation after 28 days. Therefore, the test substance is considered to be ''Not Readily Biodegradable'.
- Executive summary:
The study was performed to asses the ready biodegradability of the test substance. The study was conducted in accordance with 92/69/EEC Method C.4-E: Closed Bottle Test. This test method is in all essential parts identical with OECD Guideline 301 D. The test substance (5.9 mg/L nominal concentration) in a mineral medium was inoculated with secondary effluent and incubated for 28 days under aerobic conditions in completely full, closed bottles. During this period the biodegradation is followed by analysis of dissolved oxygen. The amount of oxygen taken up by the test chemical, corrected for uptake by the blank inoculum run in parallel, is expressed as a percentage of theoretical oxygen demand (ThOD). The test substance showed 1%, 1%, 0%, and 2% degradation after 7, 14, 21, and 28 days respectively. Therefore, the test substance is considered to be 'Not Readily Biodegradable'. The used concentration of the test substance did not show toxic effects to bacteria.
Reference
Validity criteria for the measurement of the biodegradation according to OECD 301:
Target condition according to guideline: | Actual condition according to the study: | Validity criteria met: |
In order to check the procedure, reference compounds which meet the criteria for ready biodegradability are tested by setting up an appropriate vessel in parallel as part of normal test runs. Suitable compounds are aniline (freshly distilled), sodium acetate and sodium benzoate. | Benzoic acid, sodium salt was used as a reference compound. | Yes |
A test is considered valid if the difference of extremes of replicate values of the removal of the test chemical at the plateau, at the end of the test or at the end of the 10-d window, as appropriate, is less than 20% and if the percentage degradation of the reference compound has reached the pass levels by day 14. | The % degradation of the two measured values of the test substance at the end of the test (28 d) range between 1 - 2% There was 73% degradation of the reference substance after 14 d - the pass level (60 % for ThOD) was reached. | Yes |
If in a toxicity test, containing both the test substance and a reference compound, less than 35% degradation (based on total DOC) or less than 25% (based on total ThOD or ThCO2) occurred within 14 days, the test substance can be assumed to be inhibitory. The test series should be repeated, using a lower concentration of test substance (if this can be done without seriously impairing the accuracy of the DOC determination) and/or a higher concentration of inoculum, but not greater than 30 mg solids/L. | The used concentrations of the test substance did not show toxic effects to bacteria (after 7, 14, 21 and 28 d, 28%, 29%, 31%, and 32% degradation were measured based on ThOD). | Yes |
Description of key information
Not readily biodegradable: 2% after 28 d
Key value for chemical safety assessment
- Biodegradation in water:
- not biodegradable
- Type of water:
- freshwater
Additional information
A study was performed to asses the ready biodegradability of the test substance. The study was conducted in accordance with 92/69/EEC Method C.4-E: Closed Bottle Test. This test method is in all essential parts identical with OECD Guideline 301 D. The test substance (5.9 mg/L nominal concnetration) in a mineral medium was inoculated with secondary effluent and incubated for 28 days under aerobic conditions in completely full, closed bottles. During this period the biodegradation is followed by analysis of dissolved oxygen. The amount of oxygen taken up by the test chemical, corrected for uptake by the blank inoculum run in parallel, is expressed as a percentage of theoretical oxygen demand (ThOD). The test substance showed 1%, 1%, 0%, and 2% degradation after 7, 14, 21, and 28 days respectively. Therefore, the test substance is considered to be 'Not Readily Biodegradable'.The used concentration of the test substance did not show toxic effects to bacteria.
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