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EC number: 205-352-0 | CAS number: 139-08-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From May 16, 2007 to May 17, 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Identity: Barquat MS 100
Chemical name: N-Alkyl(C12-C14)-N,N-dimethyl-N-benzylammonium chloride (ADBAC C12-C14)
Lot number: 612212
Expiry date: 21 December 2009
Purity: 99.1% C14 ADBAC, 0.6% Free amine, 0.4% Amine hydrochloride
Appearance: White powder
Storage conditions: Room temperature
Stability: The a.i., ADBAC, is hydrolytically and photolytically stable under the conditions of this study and has been shown to be stable in aqueous, alcohol and alcohol/aqueous solutions for extended periods, e.g. at least five years under standard laboratory conditions - Analytical monitoring:
- no
- Vehicle:
- yes
- Remarks:
- Water
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Preparation of the microbial inoculum
A sample of activated sludge was obtained the day before the start of the test from Worlingworth Sewage Treatment Works, which treats predominantly domestic waste. In the laboratory, the samples were maintained under aerobic conditions at 20 ± 2ºC until required. The concentration of suspended solids in a homogenised sub-sample was determined on the day of collection and immediately before the start of the test. On the day of collection, an aliquot (10 mL) of the sludge was filtered through a dried and preweighed Whatman GF/C filter, which was then dried again at approximately 105ºC for at least one hour, allowed to cool in a desiccator and reweighed. The mixed liquor suspended solids (MLSS) content of the activated sludge was then calculated. Synthetic sewage (50 mL/L) was added to the stock of activated sludge, which was aerated overnight. On the day of the test, the MLSS content of the sludge was determined by filtering the sludge in triplicate, and adjusted to 4 g/L by the addition of tap water and the pH measured. - Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 30 min
- Remarks on exposure duration:
- Control and test substance cultures exposure: 30 minutes
- Test temperature:
- 19.7-20.1
- pH:
- 7.4-7.7
- Dissolved oxygen:
- 2.5-9.1% mgO2/L
- Nominal and measured concentrations:
- 0.8, 2.4, 7.8, 25 and 80 mg/L - Test substance
3, 10 and 32 mg/L - Reference substance - Details on test conditions:
- Dilution water
The dilution water used to prepare solutions of synthetic sewage, reference substance and mixtures was tap water that had been softened and purified using reverse osmosis, RO (Prima 4 reverse osmosis unit). This equates to Type IV water (ASTM D1193-06) and Grade 3 water (BS 3978).
Synthetic sewage
Synthetic sewage feed (stored at ca. 4ºC in the dark for no more than 5 days) for activated sludge was prepared by dissolving the following in one L of RO water:
peptone - 16.0 g
meat extract - 11.0 g
urea - 3.0 g
sodium chloride - 0.7 g
calcium chloride dihydrate - 0.4 g
magnesium sulphate heptahydrate - 0.2 g
di-potassium hydrogen phosphate - 2.8 g - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol (3,5-DCP), Purity: 99.9% (product code 113610100, batch number A0229627) was obtained from ACROS Organics.
- Key result
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 27.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Inhibition on respiration rate
- Remarks on result:
- other: 95% confidence limits: 23.4 – 31.7 mg/L
- Key result
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 10.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Inhibition on respiration rate
- Remarks on result:
- other: 95% confidence limit: 9.2 – 12.9 mg/L
- Results with reference substance (positive control):
- The thirty-minute EC50 for 3,5-DCP (10.9 mg/L) fulfilled the validity criterion relating to sensitivity to inhibition (acceptable EC50 range 5 to 30 mg/L). The validity criterion relating to the respiration rates in the control (variation not greater than 15%) was also satisfied.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under study conditions, the 30 min EC50 of test substance was found to be 27.3 mg/L (95% confidence limits, 23.4 – 31.7 mg/L).
- Executive summary:
A study was conducted to determine the effect of test substance, C14 ADBAC (purity: 99.1%), on the respiration rate of activated sludge according to the OECD Guideline 209 and EU method C.11, in compliance with GLP. Samples of activated sludge (suspended solids 1.6 g/L) fed with synthetic sewage, were exposed to the test substance at nominal concentrations of 0.8, 2.4, 7.8, 25 and 80 mg/L for 30 minutes. Their rates of oxygen consumption were determined and compared with those of controls, containing activated sludge and synthetic sewage alone, which were established at the beginning and end of the culture series. The reference inhibitor 3,5-dichlorophenol (3,5-DCP) was employed at 3, 10 and 32 mg/L, as a positive control. The specific respiration rate of the control culture established at the end of the test series (23.7 mgO2/g/h) was 102% of the rate of that established at the start (23.3 mgO2/g/h). The thirty minute 50% effect concentration (EC50) for 3,5-DCP was calculated to be 10.9 mg/L (95% confidence limits 9.2 – 12.9 mg/L). These results show that the test was valid and that the sample of activated sludge employed was sensitive to inhibition. Concentration-related inhibition of respiration rates was seen at test substance concentrations greater than 2.4 mg/L. The highest tested concentration (80 mg/L) caused 85% inhibition. The EC50 of test substance was calculated to be 27.3 mg/L (95% confidence limits, 23.4 – 31.7 mg/L). The EC20 and EC80 values were calculated to be 10.9 and 68.3 mg/L, with 95% confidence limits of 8.1 – 14.1 and 54.8 – 87.5 mg/L, respectively. Under study conditions, the 30 min EC50 of test substance was found to be 27.3 mg/L (Barnes, 2007).
Reference
Description of key information
Based on the study results, the 30 min EC50 of test substance for inhibition of respiration rate of activated sludge was found to be 27.3 mg/L (nominal).
Key value for chemical safety assessment
- EC50 for microorganisms:
- 27.3 mg/L
Additional information
A study was conducted to determine the effect of test substance, C14 ADBAC (purity: 99.1%), on the respiration rate of activated sludge according to the OECD Guideline 209 and EU method C.11, in compliance with GLP. Samples of activated sludge (suspended solids 1.6 g/L) fed with synthetic sewage, were exposed to the test substance at nominal concentrations of 0.8, 2.4, 7.8, 25 and 80 mg/L for 30 minutes. Their rates of oxygen consumption were determined and compared with those of controls, containing activated sludge and synthetic sewage alone, which were established at the beginning and end of the culture series. The reference inhibitor 3,5-dichlorophenol (3,5-DCP) was employed at 3, 10 and 32 mg/L, as a positive control. The specific respiration rate of the control culture established at the end of the test series (23.7 mgO2/g/h) was 102% of the rate of that established at the start (23.3 mgO2/g/h). The thirty minute 50% effect concentration (EC50) for 3,5-DCP was calculated to be 10.9 mg/L (95% confidence limits 9.2 – 12.9 mg/L). These results show that the test was valid and that the sample of activated sludge employed was sensitive to inhibition. Concentration-related inhibition of respiration rates was seen at test substance concentrations greater than 2.4 mg/L. The highest tested concentration (80 mg/L) caused 85% inhibition. The EC50 of test substance was calculated to be 27.3 mg/L (95% confidence limits, 23.4 – 31.7 mg/L). The EC20 and EC80 values were calculated to be 10.9 and 68.3 mg/L, with 95% confidence limits of 8.1 – 14.1 and 54.8 – 87.5 mg/L, respectively. Under study conditions, the 30 min EC50 of test substance for inhibition of respiration rate of activated sludge was found to be 27.3 mg/L (Barnes, 2007).
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