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EC number: 212-985-6 | CAS number: 901-44-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2005
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- None.
- Analytical monitoring:
- yes
- Details on sampling:
- Samples were taken from the test water (about 1 mL) for each concentration at the start of the experiment, before and after water replacement (48 hour), as well as at the conclusion of the experiment. 300 µL of acetonitrile were added to 300 µL of each of the test samples prior to HPLC analysis, which was conducted immediately after sampling.
- Vehicle:
- no
- Details on test solutions:
- 80mg of the test substance were weighed, added to 2L of dilution water and stirred for 48 hours and 15 minutes. Furthermore, ad-hoc dissolution work was performed ultrasonically (about 4×5 minutes) during the stirring period. Filtering (GA55,90mm, 0.6µm) was performed after the conclusion of stirring and this filtrate was treated as the stock solution. The tested substance concentration of this stock solution was measured and this was treated as the saturation concentration (28.7 mg/L). Dilution water into which the tested substance had not been added was used for the control group. The preparation of the test medium was performed just prior to the start of the trial. Tested concentrations were all prepared by dilution of the stock solution.
- Test organisms (species):
- Oryzias latipes
- Details on test organisms:
- The testing used Oryzias latipes purchased from a vendor. The total length of the test fish was 2.3±1.2cm.
Supplier: Name: Araya Fish Farm
Address: Kyujo-cho 1061, Yama-city, Yamato-Gun, Nara Prefecture, Japan
Domestication and acclimation were performed for 32 days under the same conditions as the test conditions (water quality, temperature, etc.), and using water equivalent to that used in the test. Feed “Oryzias latipes Feed (Kyurin)” was given. No feeding was performed for 24 hours before the start of the experiment. For this test, healthy and visibly normal individuals were selected and used. The mortality rate 7 days before experiment start was 3.4% (i.e. less than 5%). The batch was therefore deemed as usable for testing.
Test fish were selected from the acclimated fish at the start of the experiment. In addition, 7 fish were selected in the same manner, and their weights and lengths measured. The weights were 0.22±0.05 g, and the lengths were 2.9±0.1 cm.
In addition, the test fish (acclimation lot no.: M05C-01) underwent qualification testing with a standard substance (pentachlorophenol sodium salt). The 96-hour LC50 satisfied the range of the standard (0.3~0.7 mg/L). The resistance of this lot was determined to be within the normal range, and so these were provided for testing.
The test values performed in 28 March, 2005 – 1 April, 2005 are shown below: 96-hour LC50 (test value): 0.41 mg/L (Background Data: 0.44±0.09 mg/L) - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Remarks on exposure duration:
- None.
- Post exposure observation period:
- None.
- Hardness:
- Not measured
- Test temperature:
- 23.9°C - 24.3°C (min - max)
- pH:
- 7.5 - 7.7 (min - max)
- Dissolved oxygen:
- 6.4 - 8.1 (min - max)
- Salinity:
- Freshwater
- Conductivity:
- Not measured.
- Nominal and measured concentrations:
- Nominal concentrations: 0 ; 2.7 ; 4.9 ; 8.9 ; 15.9 ; 28.7 mg/L (dilutions were made from a unique stock solution at 28.7 mg/L and a spacing factor of 1.8)
Measured concentrations: 0 ; 3.2 ; 5.7 ; 10.0 ; 16.6 ; 34.0 mg/L
Results were based on nominal concentrations because measured concentrations were in the [80-120]% range of the nominal concentrations. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 3-L capacity glass vessels
- Material, size, headspace, fill volume: filled with at least 1 L per gram of fish wet weight
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM
Dechlorinated water (tap water processed with activated charcoal, removing residual chlorine, and fully aerated) was used. When using the dilution water, the presence of residual chlorine was checked by the o-toluidine method. The quality of the dilution water was checked twice a year.
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16L:8D
- Light intensity: Not measured
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality
The number of dead Oryzias latipes was checked at experiment start, and after 3, 24, 48, 72, and 96 hours together with recording the observed signs of toxicity or abnormalities. In cases where dead individuals were discovered, these were removed immediately so as not to degrade water quality. - Reference substance (positive control):
- yes
- Remarks:
- Pentachlorophenol sodium salt
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 20.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Details on results:
- Data on measured concentrations and mortality recorded per day are given in table 1. LC50 was determined by the Probit method. LC50 = 20.8 mg/L
- Results with reference substance (positive control):
- The test fish (acclimation lot no.: M05C-01) underwent qualification testing with a standard substance (pentachlorophenol sodium salt). The 96-hour LC50 satisfied the range of the standard (0.3~0.7 mg/L). The resistance of this lot was determined to be within the normal range, and so these were provided for testing. The test values performed in 28 March, 2005 – 1 April, 2005 are shown below: 96-hour LC50 (test value): 0.41 mg/L (Background Data: 0.44±0.09 mg/L)
- Sublethal observations / clinical signs:
Table 1: fish mortality and mean measured concentrations during the experiment
Nominal concentrations (mg/L)
Mean measured concentrations (mg/L)
Cumulative number of dead fish
24-hour
48-hour
72-hour
96-hour
Control
0
0
0
0
0
2.7
3.15
0
0
0
0
4.9
5.65
0
0
0
0
8.9
9.99
0
0
0
0
15.9
16.62
1
1
1
1
28.7
33.98
6
7
7
7
- Validity criteria fulfilled:
- yes
- Conclusions:
- A GLP test following OECD 203 guideline was conducted on Oryzias latipes under semi-static conditions (48-hour renewal). Tested concentrations were 0 ; 2.7 ; 4.9 ; 8.9 ; 15.9 ; 28.7 mg/L. They were in agreement with measured values at 0, 48 hours (both new and old) and 96 hours. The 96h-LC50 was 20.8 mg/L.
- Executive summary:
A GLP test following OECD 203 guideline was conducted on Oryzias latipes under semi-static conditions (48-hour renewal). One vessel by concentration was used, in each of which were put seven fish. Tested concentrations were 0 ; 2.7 ; 4.9 ; 8.9 ; 15.9 ; 28.7 mg/L. They were in agreement with measured values at 0 (new), 48 hours (both new and old) and 96 hours (old). Mortality was checked every 24 hour. All fish died in the 28.7 mg/L treatment. One fish died in the 15.9 mg/L treatment. No fish died in any other treatment, including control. The 96h-LC50 was calculated as 20.8 mg/L.
Reference
Description of key information
A GLP test following a protocol equivalent to OECD 203 guideline was conducted on Oryzias latipes under semi-static conditions (48-hour renewal). One vessel by concentration was used, in each of which were put seven fish. Tested concentrations were 0 ; 2.7 ; 4.9 ; 8.9 ; 15.9 ; 28.7 mg/L. They were in agreement with measured values at 0 (new), 48 hours (both new and old) and 96 hours (old). Mortality was checked every 24 hour. All fish died in the 28.7 mg/L treatment. One fish died in the 15.9 mg/L treatment. No fish died in any other treatment, including control. The 96h-LC50 was calculated as 20.8 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 20.8 mg/L
Additional information
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