Chlorobenzene

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1960

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Well documented study report which meets basic scientific principles.

Data source

Reference

Reference Type:

other company data

Title:

Unnamed

Year:

1960

Report date:

1960

Materials and methods

Principles of method if other than guideline:

BASF test: A single dose of the test substance was injected in to the peritoneal cavity of the animals. After application, the animals were observed for mortality and clinical signs of toxicity. Animals that died during the experiment were necropsied. Surviving animals were sacrificed one week after application for the purpose of necropsy.

GLP compliance:

no

Remarks:

- GLP was not mandatory at the time when study was performed.

Test material

Test material information

Constituent 1

Reference substance name:

Chlorobenzene

EC Number:

203-628-5

EC Name:

Chlorobenzene

Cas Number:

108-90-7

Molecular formula:

C6H5Cl

IUPAC Name:

chlorobenzene

Details on test material:

Name: Chlorbenzol
Purity: no data

Test animals

Species:

mouse

Strain:

other: Tübinger

Sex:

female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Designation: Tübinger maeuse
- Weight range at study initiation: 18.5 - 31g

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

olive oil

Details on exposure:

The test substance was administered as a 2%, 5% and 10% solution in olive oil. Application volume was between 8 - 25 ml/kg bw.

Doses:

0.20; 0.40; 0.80; 1.25; 1.60 ml/kg bw (222.5; 445; 890; 1,390.6; 1,780 mg/ml)

No. of animals per sex per dose:

0.20 ml/kg bw: 5 animals
0.40 ml/kg bw: 10 animals
0.80 ml/kg bw: 10 animals
1.25 ml/kg bw': 5 animals
1.60 ml/kg bw: 5 animals

Control animals:

no

Statistics:

On the basis of observed lethality, the LD50 was estimated using graphical evaluation of the dose response curve on probability paper.

Results and discussion

Effect levels

Sex:

female

Dose descriptor:

LD50

Effect level:

ca. 890 mg/kg bw

Remarks on result:

other: Corresponds to approx. 0.80 ml/kg bw

Mortality:

A concentration dependency of mortality was observed.
- 0.2 ml/kg bw: 0/5
- 0.4 ml/kg bw: 0/5
- 0.8 ml/kg bw: 5/10 (death occured between 48-72h).
- 1.25 ml/kg bw: 7/10 (6 animals within 48h. 7th animals within 72h).
- 1.6 ml/kg bw: 5/5 (4 animals died within 20h after application. 5th animal between 48h and 72h.

Clinical signs:

- 0.2 ml/kg bw: Shabby fur, adhered eyelids.
- 0.4 ml/kg bw: Agitatation on day of application, otherwise, no other abnormality was detected.
- 0.8 ml/kg bw: On the day of application, the animals were groggy, very agitated and had accelerated irregular respiration. On the following day, the animals had a shabby fur, partially adhered eyelids and displayed grogginess and apathy.
- 1.25 ml/kg bw: On day of application, the animals experienced excitement, tremor, grogginess and slight abdominal position. On the following day, a shabby fur glued together by the test substance was observed.
- 1.6ml/kg bw: 5 minutes after application, grogginess and severe tremor was observed. After 20 mins, abdominal positioning partly merged with lateral positioning was observed. 2 1/2 h post application, the symptoms still persisted. In addition, accelerated respiration was also observed. 20 h post application, following symptoms were still observable in the surviving animals: severe tremor, adhered eyes, oily fur, intermittent respiratation, grogginess and slight abdorminal position. In the following days, the fur of the animals were shabby and glued with the test substance.

Gross pathology:

The following findings reported were observed only in those animals that perished in the study. No abnormality was found in the organs (liver, kidney, spleen) of the animals that were sacrificed one week after application of the test substance.
0.8 ml/kg bw (5 deaths): slight liver decolouration (2/5), reddened small intestine (2/5), kidney decolouration (1/5)
1.25 ml/kg bw: (7 deaths): liver (light red), reddened small intestine, decolouration of kidney (6/7)
1.6ml/kg bw: (5 deaths): slightly reddened small intestine (1/5), decolouration of organs (3/5)