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Diss Factsheets
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EC number: 206-114-9 | CAS number: 302-01-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Direct weighings were prepared to give the different test item concentrations. The test item was added into Erlenmeyer flasks (incubation vessels) to about 130 mL deionised water
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20 +/- 2 °C
- pH:
- 7.6 to 7.9
- Nominal and measured concentrations:
- range-finding test: 10, 100, 1000 mg/L (nominal)
main-test: 5.6, 10, 18, 32, 56 mg/L (nominal) - Details on test conditions:
- EXPERIMENTAL PROCEDURE
- 250 mL of sludge with test item (or control or reference compound) was incubated for 3 h in 300 mL closed Erlenmeyer flasks (with air inlet and outlet) and aerated through a glass tube at 50-100 L/h with clean oil-free air.
- the content of the Erlenmeyer flasks was completely transferred to 250 mL BOD bottles and O2-content was measured with an O2-meter (redox electrode) with writer.
- Two controls without test item were included in the test design, one at the start and the other at the end of the test series.
- reference compound: 3,5-Dichlorophenol
- physico-chemical oxygen consumption control: to differentiate between physico-chemical oxygen consumption and biological oxygen consumption (respiration), at least the maximum concentration of the test item was tested without activated sludge.
- The respiration rate for each concentration was determined graphically from the linear part of the curve of O2-content versus time. The inhibitory effect of the test item at a particular concentration is expressed as a percentage of the mean of the respiration rates of two controls. An EC50 value was calculated from the respiration rates at different test item concentrations. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 8.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: referring to hydrazine hydrate (containing 64 % hydrazine); 95 % CI: .7 to 11.4 mg/L
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 5.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: referring to 100 % hydrazine
- Validity criteria fulfilled:
- yes
- Remarks:
- All validity criteria of the test method were met: - respiratory rates of the 2 controls differ less than 15 % from each other - the EC50 of the reference compound 3,5-Dichlorophenol is in the range 5 – 30 mg/L
- Executive summary:
The toxicity of hydrazine towards microorganisms is characterized by an EC50(3 h) of 5.5 mg/L (nominal) testing the respiration rate of activated sludge (Currenta, 2010c).
Reference
Description of key information
The toxicity of hydrazine towards microorganisms is characterized by an EC50(3 h) of 5.5 mg/L (nominal) testing the respiration rate of activated sludge (Currenta, 2010c).
Key value for chemical safety assessment
- EC50 for microorganisms:
- 5.5 mg/L
Additional information
According to the Guidance document R.7b (ECHA, 2008), preference is given to tests with a mixed inoculum that assess the functioning of the entire microbial community in a sewage treatment plant, rather than tests based on single species or even microbial sub-systems. Respirometry is generally considered as an approach that will integrate the functioning of all organisms in an STP. Concluding, Currenta (2010c) was chosen to represent the toxicity of the test item towards microorganisms and thus the functioning of the sewage treatment plant, as all reported supporting studies performed by Bringmann et al. used single species as test organisms. These species represent only a minor part of microorganisms present in sewage treatment systems and should only be used for the risk assessment in the absence of other reliable data.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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