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EC number: 206-114-9 | CAS number: 302-01-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Deviations:
- yes
- Remarks:
- The fluctuation of the temperature during the test was slightly higher than 1°C. This is not regarded to be relevant to the results.
- Principles of method if other than guideline:
- The method described in the Council Regulation (EC) No 440/2008, Method C.20 'Daphnia magna Reproduction Test" (2008), which equals in all relevant parts to the OECD Test guideline 211 (Daphnia magna Reproduction Test; adopted: Oct. 3, 2008), assesses adverse effects of various concentrations of a test item to a freshwater microcrustacean species within a 21 day exposure period under semi-static conditions.
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Routinely the samples are analysed immediately. Only in exceptional cases, they are stored in a refrigerator overnight and protected from light.
- Sampling schedule:
Control :
on day 0, 7 and 14 in the freshly prepared media;
on day 2, 9 and 16 in the old media
Test concentrations :
on day 0, 7 and 14 in the freshly prepared media;
on day 2, 9 and 16 in the old media - Vehicle:
- no
- Details on test solutions:
- A stock solution was prepared to give the desired series of test concentrations. To achieve this 19.4 µL (= 20 mg) of the test item were added to 2 litres of dilution water. The pH was measured.
To produce the different test item concentrations appropriate amounts of the stock solution were diluted with dilution water to a volume of 100 mL per replicate. For each test item concentration and control 10 replicates were prepared. This procedure was repeated three times a week at each water renewal. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Name: Daphnia magna STRAUS, parthenogenetic females
Source: Strain of Bundesgesundheitsamt Berlin
Maintenance and Acclimatisation : A population of parthenogenetic females of synchronized age structure - Test type:
- semi-static
- Water media type:
- freshwater
- Total exposure duration:
- 21 d
- Hardness:
- The hardness of the dilution water used ranged from 13.5 – 15.8 °dH (= 241 - 282 mg/L CaCO3) during the test.
- Test temperature:
- 19.9 °C - 21.9 °C
- pH:
- 7.9 - 8.2
- Dissolved oxygen:
- 8.5 - 10.5 mg/L
- Nominal and measured concentrations:
- The results are expressed in terms of time-weighted means of measured concentrations. Effective concentrations ranged from 78.1 – 170 % of the nominal values in the freshly prepared media and from 17.0 – 110 % of the nominal values in the media after 48 hours, or 72 hours of exposure, respectively.
- Details on test conditions:
- Test vessels: 150 mL glass beakers holding 1 neonate in 100 mL of test medium
Experimental design: 5 test concentrations plus 1 control, 1 neonate per vessel, 10 replicates per concentration/control, semi-static system (renewal of the test media on Monday, Wednesday and Friday) respectively
Method of initiation: neonates were placed in prepared media
Photoperiod: 16 h light: 8 h dark
Light intensity: 15 - 20 µE x m-2 x s-1
Temperature of incubation unit: 20.0 to 21.0 °C (measured in air)
Aeration: none
Test item concentration/s (nominal): 0.01, 0.032, 0.1, 0.32 and 1 mg/L
Food source and : Desmodesmus subspicatus
feeding rate: day 0-7: 0.1 mg C / Daphnia / day
day 8-21: 0.2 mg C / Daphnia / day
Duration of exposure: 21 days
Criteria of effects: The criterion of adverse effects used in this study was the item-induced inhibitory effect on the parthenogenetic reproduction rate and the mobility behaviour/mortality rate of parent Daphnia, all effects being assessed at least three times a week.
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.01 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- act. ingr.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.031 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- act. ingr.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.123 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.662 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Details on results:
- - 1st brood on day 9th
- all parenteral daphnids died at nominal concentrations of 1 mg/L
- test item concentrations were monitored throughout the test duration. As effective concentrations ranged from 78.1 – 170 % of the nominal values in the freshly prepared media and from 17.0 – 110 % of the nominal values in the media after 48 hours, or 72 hours of exposure, respectively, the test evaluation was based on time-weighted means of the measured concentrations. - Validity criteria fulfilled:
- yes
- Remarks:
- The mortality rate in the controls did not exceed 20 % by the end of the test. Living offspring produced per parent Daphnia surviving at the end of the test was > 60 in the controls. The dissolved oxygen concentration remained above 3 mg/L throughout the
- Executive summary:
The long-term toxicity of hydrazine towards invertebrates was measured yielding a NOEC(21 d) of 0.010 mg/L having reproduction as most sensitive end point (Currenta, 2010b). The test evaluation was based on time-weighted means of the measured concentrations.
Reference
Description of key information
The long-term toxicity of hydrazine towards invertebrates was measured yielding a NOEC(21 d) of 0.010 mg/L having reproduction as most sensitive end point (Currenta, 2010c). The test evaluation was based on time-weighted means of the measured concentrations.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.01 mg/L
Additional information
As hydrazine is known to rapidly degrade in aqueous media, tests were conduced under semi-static conditions refreshing aqueous solutions each Monday, Wednesday and Friday to ensure a constant exposure of the test animals throughout the entire test duration. Furthermore, concentrations were measured on day 0, 7 and 14 in the freshly prepared media; and on day 2, 9 and 16 in the old media by the means of a photometric method to determine the effective exposure concentration. Effective concentrations ranged from 78.1 – 170 % of the nominal values in the freshly prepared media and from 17.0 – 110 % of the nominal values in the media after 48 hours, or 72 hours of exposure, respectively. Therefore, the test evaluation was based on time-weighted means of the measured concentrations.
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