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EC number: 221-201-1 | CAS number: 3030-47-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study with acceptable restrictions (no details on test substance, e.g. purity, no data on individual animals availabvle; limited results description - body weight data missing, DPM values not available)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
- GLP compliance:
- yes
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- Bis(2-dimethylaminoethyl)(methyl)amine
- EC Number:
- 221-201-1
- EC Name:
- Bis(2-dimethylaminoethyl)(methyl)amine
- Cas Number:
- 3030-47-5
- Molecular formula:
- C9H23N3
- IUPAC Name:
- (2-{[2-(dimethylamino)ethyl](methyl)amino}ethyl)dimethylamine
- Test material form:
- not specified
- Details on test material:
- Test material (CAS #3030-47-5) obtained from Air Products and Chemicals, Lot #AW411814M4
Constituent 1
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- Balb/c
- Sex:
- not specified
- Details on test animals and environmental conditions:
- Supplier of animals: Charles River
The age of the mice ranged from 9 to 11 weeks.
All animals were observed cageside at least twice daily for morbidity, mortality, availability of food and water and treatment effects.
These observations included evaluation of the skin, fur and mucous membranes, respiration, nervous system and behavior patterns.
The animal rooms of the testing facility were designed to maintain adequate environmental conditions concerning temperature, humidity, and
photocycle and are regulated for the species under test. The temperature was set at 22 +/- 1 °C, the humidity at 50 +/- 10% RH and the
lightldark cycle is set at 12 hours on: 12 hours off.
Feed analysis was performed by Purina Mills, Inc. and analysis of the tap water (municipal water supply) was performed in
accordance with Laboratory Standard Operating Procedures.
Study design: in vivo (LLNA)
- Vehicle:
- acetone/olive oil (4:1 v/v)
- Concentration:
- 1%
- No. of animals per dose:
- 5
- Details on study design:
- Study consisted of a vehicle control group (5 mice treated with a solution of 4:l acetone:olive oil, AOO), three positive control
groups (5 mice treated with a solution of each 1.0% dinitrochlorobenzene (DNCB), 0.17% benzalkonium chloride (BC), and 25% trimellitic
anhydride (TMA), and treatment groups (5 mice treated with solutions of the test substance in AOO). The test substance was tested in
the LLNA at the minimal irritating concentration (MIC) determined in previous Primary Dermal Irritation Studies (PDIS).
Prior to the application of the solutions on Day 1, an erythema score was assessed for each mouse. The solutions were applied by
dispensing 12.5 ul of the appropriate solution onto the dorsal and ventral side of each ear - each ear received a total of 25 ul of solution for a total of 50 ul per mouse. The solutions were applied once daily for three consecutive days. On Day 5, the erythema score was assessed for each mouse. Each mouse was then injected, via the lateral tail vein, with 0.2 mi of phosphate buffered saline (PBS)
containing 20 uCi of 3H-thymidine. Five hours after the injections, the mice were sacrificed by C02 inhalation and the draining auricular
lymph nodes were excised and pooled for each mouse. A single cell suspension of lymph node cells (LNC) was prepared and
3H-thymidine incorporation was measured on a beta-scintillation counter. Radioisotope incorporation was measured as disintegrations per minute (dpm) per mouse and a mean dpm value +/- SE (standard error) was calculated for each experimental group.
In addition, a stimulation index (SI) was calculated using absolute dpm value for each mouse as the numerator, and the mean dpm value
from the vehicle control mice as the denominator. A mean SI k SE was calculated for each experimental group. Any chemical that produces a SI of > 3 in the LLNA was considered "positive". - Statistics:
- Calculations were made using Microsoft Excel. The following equations were used:
1. SI = dpm of mouse / mean dpm of vehicle group
2. AVERAGE* = "average" of numbers
3. STDEV* = "estimates standard deviation of a population based on a sample"
4. Standard Error (SE) = STDEV s the square root of the number of mice in group
* Defined by Microsoft Excel
Results and discussion
- Positive control results:
- Trial 1:
TMA, but not DNCB produced a robust response. DNCB failed to trigger a SI well in excess of 3.0 in Trial 1.
BC triggered a slight SI, which was less than 3.0 and consistent with the fact that it is an irritant; but not a sensitizer.
Trial 2:
SI of 2.80 for 0.17% BC, SI of 46.10 for 1.0% DNCB, and SI of 44.76 for 25% TMA.
In vivo (LLNA)
Resultsopen allclose all
- Parameter:
- SI
- Remarks on result:
- other: A mean SI +/- SE was calculated for the test material from two separate trials Trial 1: SI: 3.53 +/- 0.50 Trial 2: SI: 1.61 +/- 0.32 Overall mean: SI: 2.57 +/- 0.42.
- Parameter:
- other: disintegrations per minute (DPM)
- Remarks on result:
- other: data not available
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Remarks:
- Migrated information
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