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EC number: 201-058-1 | CAS number: 77-78-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 21.08.1990 to 23.08.1990
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable, well-documented publication/study report which meets basic scientific principles. No determination of the test substance concentration, therefore no method validation included in the study.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- No determination of the test substance concentration, therefore no method validation included in the study.
- GLP compliance:
- not specified
- Remarks:
- The test was performed in 1990 by Stuhlfauth. Only the report was newly generated at the mentioned date given above, form archived laboratory manuscripts.
Test material
- Reference substance name:
- Dimethyl sulphate
- EC Number:
- 201-058-1
- EC Name:
- Dimethyl sulphate
- Cas Number:
- 77-78-1
- Molecular formula:
- C2H6O4S
- IUPAC Name:
- dimethyl sulfate
- Details on test material:
- - Name of test material (as cited in study report): Dimethyl sulphate
- Physical state: liquid
- no further significant data stated
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
- not stated
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- no data
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Stock solution: 2048 mg DMS/L
- no further significant data stated
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Number of animals: 20 per test concentration (devided into 2 groups of 10)
- Common name: Daphnia
- Age at study initiation (mean and range, SD): 3- 24 h
- Method of breeding: Adult animals were cultivated in glass vessels (volume: 4 litres). The medium was changed once a week. Twice daily the animals were fed with algae (Scenedesmus supspicatus).
- control group: animals in medium without test substance
- no further significant data stated
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- no
Test conditions
- Hardness:
- no data
- Test temperature:
- 21 °C
- pH:
- no data
- Dissolved oxygen:
- after 48 h: 9.5 mg/L
- Salinity:
- no data
- Nominal and measured concentrations:
- nominal concentrations: 0, 2, 4, 8, 16, 32, 64, 128, 256, 512, 1024 mg/L; separation factor 2
- Details on test conditions:
- TEST SYSTEM
- test volume: 50 ml
- Test vessel:
- Material, size, headspace, fill volume: 100 ml glass vessel
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Medium: synthetic water according to DIN 38 412 Part 11
- Source/preparation of dilution water: synthetic water
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod:dark chamber
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- determination of mobility after 24 and 48 hours
TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study: yes
- Results used to determine the conditions for the definitive study: With the indicated test data the EC50 of Dimethylsulfate TTR (tested batch) to Daphnia magna under the conditions of the test was estimated to be 17 mg/l based on nominal values. No immobilization was observed at the two lowest test concentrations of 2 mg/l and 4 mg/l.
- no further details stated - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate, 0.9 and 1.9 mg/l, 2 replicates
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 17 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 14-21 mg/L
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 64 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 53 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Remarks on result:
- other: 41-68 mg/L
- Duration:
- 24 h
- Dose descriptor:
- EC0
- Effect conc.:
- 4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC100
- Effect conc.:
- 128 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- Mortality of control: below 10 % immobilised
- Results with reference substance (positive control):
- Mortality (Potassium dichromate)
EC50: after 24 h was between 0.9 and 1.9 mg/L
no further details stated - Reported statistics and error estimates:
- Probit analysis
Any other information on results incl. tables
Immobilization rates of Daphnia magna exposed to Dimethylsulfate TTR over a period of 24 and 48 hours
Nominal test concentration |
Replicate |
Number of daphnids exposed |
Number of immobilized daphnids |
Percentage of immobilized daphnids |
Mean [%] |
|||
24 h |
48 h |
24 h |
48 h |
24 h |
48 h |
|||
Control |
1 |
10 |
0 |
0 |
0 |
0 |
0 |
5 |
2 |
10 |
0 |
1 |
0 |
10 |
|||
2 |
1 |
10 |
0 |
0 |
0 |
0 |
0 |
0 |
2 |
10 |
0 |
0 |
0 |
0 |
|||
4 |
1 |
10 |
0 |
0 |
0 |
0 |
0 |
0 |
2 |
10 |
0 |
0 |
0 |
0 |
|||
8 |
1 |
10 |
0 |
0 |
0 |
0 |
5 |
0 |
2 |
10 |
1 |
0 |
10 |
0 |
|||
16 |
1 |
10 |
2 |
9 |
20 |
90 |
15 |
50 |
2 |
10 |
1 |
1 |
10 |
10 |
|||
32 |
1 |
10 |
2 |
9 |
20 |
90 |
15 |
90 |
2 |
10 |
1 |
9 |
10 |
90 |
|||
64 |
1 |
10 |
3 |
10 |
30 |
100 |
35 |
100 |
2 |
10 |
4 |
10 |
40 |
100 |
|||
128 |
1 |
10 |
10 |
10 |
100 |
100 |
100 |
100 |
2 |
10 |
10 |
10 |
100 |
100 |
|||
256 |
1 |
10 |
10 |
10 |
100 |
100 |
100 |
100 |
2 |
10 |
10 |
10 |
100 |
100 |
|||
512 |
1 |
10 |
10 |
10 |
100 |
100 |
100 |
100 |
2 |
10 |
10 |
10 |
100 |
100 |
|||
1024 |
1 |
10 |
10 |
10 |
100 |
100 |
100 |
100 |
2 |
10 |
10 |
10 |
100 |
100 |
Table 2: Number and percentage of immobilization of Daphnia magna after 24 hours exposed to Potassium dichromate
Nominal test concentration |
Replicate |
Number of daphnids exposed |
Number of immobilized daphnids |
Percentage of immobilized daphnids |
Mean [%] |
|||
0.9 |
1 |
10 |
1 |
5 |
10 |
50 |
10 |
55 |
2 |
10 |
1 |
6 |
10 |
60 |
|||
1.9 |
1 |
10 |
10 |
10 |
100 |
100 |
100 |
100 |
2 |
10 |
10 |
10 |
100 |
100 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 was determined at 17 mg test substance/L after 48h of exposure in freshwater. The test was performed with daphnia magna. No analytical determination was performed, the values are based on the nominal concentration. Due to the fact that DMS hydrolyses very fast (DT50 <1d) in water to monomethyl sulfate and methanol, the immobilisation observed is predominantly based on the hydrolysis products.
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