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EC number: 203-640-0 | CAS number: 109-02-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1990-10-10 - 1990-10-24
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- 4-methylmorpholine
- EC Number:
- 203-640-0
- EC Name:
- 4-methylmorpholine
- Cas Number:
- 109-02-4
- Molecular formula:
- C5H11NO
- IUPAC Name:
- 4-methylmorpholine
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): 6398-24-20, Project #90-005
- Substance type: clear, colorless liquid
- Physical state: liquid
- Stability under test conditions: There was no apparent change in the physical characteristics of the test article during administration.
- Other: specific gravity: 0.92 g/mL; pH 10.8 (taken from MSDS)
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Hare-Marland, Hewitt, New Jersey, USA
- Age at study initiation: young adult animals
- Weight at study initiation: males 2224-2691 g (mean 2430 g); females 2224-2775 g (mean 2380.6 g)
- Housing: Rabbits were housed individually in cages sized in accordance with the "Guide for the care and use of laboratory animals' of the Institute of Laboratory Animal Resources, National Research Council
- Diet (e.g. ad libitum): Purina Rabbit Ration, H.F., ad libitum, checked daily and added or replaced as needed. Feeders are designed to reduce soiling, bridging and scattering
- Water (e.g. ad libitum): fresh tap water, ad libitum
- Acclimation period: minimum of 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20°C ± 3°C
- Humidity (%): 30 to 70%
- Photoperiod (hrs dark / hrs light): 12 hours light, 12 hours dark
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- unchanged (no vehicle)
- Details on dermal exposure:
- TEST SITE
Approximately 24 hours before testing, fur was clipped from the dorsal area of the trunk of the test animals. The test article was applied directly onto the exposed intact skin of the animals taking care to spread the substance evenly over the entire area. A square gauze patch was placed on the animals to cover the dosed area. A square gauze patch was placed on the animals to cover the dosed area. The animals were wrapped with rubber dam and an elastic bandage to retard evaporation.
REMOVAL OF TEST SUBSTANCE
- Washing (if done): The test article was held in contact with the skin for twenty-four hours. Following the twenty-four hour period of exposure, the wrappings were removed. - Duration of exposure:
- 24 hours
- Doses:
- 3000 mg/kg
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Observations were recorded daily through day 14. Body weights were recorded at initation and on days 7 and 14 or upon death. All surviving rabbits were sacrificed by a lethal injection of BeuthanasiaR solution on day 14 and a gross necropsy was performed.
Results and discussion
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 3 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- Three of ten rabbits died during the study (2 males, 1 female).
- Clinical signs:
- other: Clinical signs observed during the limit test included decreased activity, decreased muscle tone, abnormal stance, abnormal gait, hind end drop and dyspnea.
- Gross pathology:
- Necropsy of the animals dying on study included pale and/or mottled liver, pale and/or mottled kidneys, dark red and mottled lungs, oral and/or nasal discharge, and severe irritation of the underlying muscle at the application site.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Based upon the observations made in this Acute Exposure Dermal Toxicity Study in rabbits, the estimated dermal LD50 for the substance was determined to be greater than 3000 mg/kg. The substance is considered not classified as acute dermal toxicant according to criteria laid down in the CLP Regulation.
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