Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 231-626-4 | CAS number: 7659-86-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 28 April to 24 June 1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2-ethylhexyl mercaptoacetate
- EC Number:
- 231-626-4
- EC Name:
- 2-ethylhexyl mercaptoacetate
- Cas Number:
- 7659-86-1
- Molecular formula:
- C10H20O2S
- IUPAC Name:
- 2-ethylhexyl 2-sulfanylacetate
- Reference substance name:
- 2-ethylhexyl thioglycolate
- IUPAC Name:
- 2-ethylhexyl thioglycolate
- Details on test material:
- - Name of test material (as cited in study report): 2-ethylhexyl thioglycolate
- Expiration date of the lot/batch: January 1994
- Storage condition of test material: at room temperature, away from light
Constituent 1
Constituent 2
Method
- Target gene:
- This test enables the detection of base-pair substitution and frameshift mutagens. Mutagenic substances can induce reversion in histidine-deficient strains, which are then able to grow and form colonies in a histidine-limited medium, while non-revertants can not.
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA98, TA100, TA102, TA1535 & TA1537
- Additional strain / cell type characteristics:
- not applicable
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix
- Test concentrations with justification for top dose:
- Without S9: 2.5, 5, 10, 20 & 50 µg/plate
With S9: 250, 500, 1000, 2000 & 4000 µg/plate (1st assay); 31.25, 62.5, 125, 250 & 500 µg/plate (2nd assay) - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: not reported but commonly used for this type of study.
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: Without S9 mix: · 1 µg/plate of sodium azide (NaN3): TA 1535 and TA 100 strains, · 50 µg/plate of 9-Aminoacridine (9AA): TA 1537 strain, · 0.5 µg/plate of 2-Nitrofluorene (2NF): TA 98 strain, · 0.5 µg/plate of Mitomycin C (MMC): TA 102 strain. With S9 mi
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation) and preincubation
- Type and identity of media:
Direct plate incorporation method:
Molten agar containing traces of histidine and biotin and maintained at 45°C. Then Petri plate containing minimum medium.
Preincubation method:
Minimum agar plate.
DURATION
- Preincubation period: 14 hours
- Exposure duration: 48 to 72 hours
SELECTION AGENT (mutation assays): Histidine
NUMBER OF REPLICATIONS: 2 independent tests, using 3 plates/concentration
DETERMINATION OF CYTOTOXICITY
- other: observation whether the bacterial lawn is sparse and whether the number of colonies is decreased. - Evaluation criteria:
- Positive response if:
- a reproducible and significant (if p <= 0.05) dose relationship, and/or
- a reproducible and significant (doubling in the number of revertants) increase
Test valid because:
- Results of the negative and vehicle controls in the range of corresponding historical data, and
- Results of the positive control higher than results of negative and vehicle controls and in the range of corresponding historical data - Statistics:
- Linear regression analysis
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: TA98, TA100, TA102, TA1535 & TA1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Without S9: >= 50 µg/plate|with S9: >= 5000 µg/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- COMPARISON WITH HISTORICAL CONTROL DATA:
The negative and solvent control results were equivalent to those usually obtained in the laboratory. The number of revertants induced by the
positive controls was higher than the controls, indicating the sensitivity of the test.
RANGE-FINDING/SCREENING STUDIES:
Precipitation was observed from 500 µg/plate in the first cytotoxicity test conducted without S9. It was not observed in the corresponding test
conducted with S9.
COMPARISON WITH HISTORICAL CONTROL DATA:
The negative and solvent control results were equivalent to those usually obtained in the laboratory. The number of revertants induced by the
positive controls was higher than the controls, indicating the sensitivity of the test. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Table 1: Mean Revertants per Plate – Direct plate incorporation method
Test Material |
TA-1535 |
TA-1537 |
TA-102 |
TA-98 |
TA-100 |
|||||
Mean |
SD |
Mean |
SD |
Mean |
SD |
Mean |
SD |
Mean |
SD |
|
Nonactivation |
||||||||||
Negative Control |
11 |
2 |
12 |
2 |
160 |
17 |
18 |
3 |
92 |
4 |
Solvent Control |
11 |
2 |
6 |
2 |
219 |
8 |
12 |
4 |
71 |
16 |
2.5 µg/plate |
13 |
1 |
9 |
1 |
196 |
16 |
18 |
1 |
80 |
5 |
5 µg/plate |
13 |
3 |
11 |
2 |
199 |
22 |
13 |
3 |
81 |
4 |
10 µg/plate |
9 |
3 |
7 |
2 |
245 |
20 |
14 |
4 |
80 |
13 |
20 µg/plate |
11 |
2 |
7 |
1 |
222 |
33 |
13 |
1 |
84 |
4 |
50 µg/plate |
5 |
1 |
8 |
2 |
226 |
8 |
17 |
2 |
86 |
12 |
Positive Control |
188 |
36 |
149 |
13 |
1005 |
236 |
109 |
10 |
323 |
23 |
Activation |
||||||||||
Solvent Control |
13 |
1 |
10 |
1 |
297 |
20 |
25 |
3 |
108 |
7 |
250 µg/plate |
13 |
4 |
8 |
1 |
261 |
14 |
24 |
4 |
101 |
12 |
500 µg/plate |
10 |
5 |
7 |
2 |
278 |
35 |
22 |
7 |
119 |
7 |
1000 µg/plate |
11 |
3 |
6 |
1 |
304 |
23 |
22 |
6 |
115 |
6 |
2000 µg/plate |
13 |
2 |
8 |
2 |
320 |
27 |
20 |
5 |
105 |
7 |
4000 µg/plate |
11 |
1 |
10 |
1 |
324 |
31 |
23 |
3 |
100 |
28 |
Positive Control |
252 |
35 |
147 |
9 |
895 |
66 |
1767 |
131 |
1573 |
43 |
Table 2: Mean Revertants per Plate – Preincubation method
Test Material |
TA-1535 |
TA-1537 |
TA-102 |
TA-98 |
TA-100 |
|||||
Mean |
SD |
Mean |
SD |
Mean |
SD |
Mean |
SD |
Mean |
SD |
|
Nonactivation |
||||||||||
Negative Control |
10 |
4 |
12 |
3 |
261 |
8 |
15 |
1 |
79 |
5 |
Solvent Control |
7 |
3 |
13 |
2 |
210 |
8 |
15 |
5 |
69 |
8 |
2.5 µg/plate |
9 |
3 |
13 |
3 |
254 |
23 |
14 |
4 |
84 |
4 |
5 µg/plate |
7 |
1 |
10 |
3 |
258 |
10 |
18 |
5 |
84 |
8 |
10 µg/plate |
6 |
3 |
13 |
2 |
264 |
11 |
17 |
2 |
74 |
7 |
20 µg/plate |
8 |
0 |
13 |
4 |
238 |
30 |
14 |
4 |
82 |
10 |
50 µg/plate |
7 |
2 |
9 |
2 |
353 |
15 |
20 |
2 |
76 |
7 |
Positive Control |
232 |
5 |
185 |
21 |
1676 |
185 |
133 |
12 |
296 |
23 |
Activation |
||||||||||
Solvent Control |
9 |
4 |
12 |
1 |
414 |
23 |
25 |
1 |
110 |
19 |
31.25 µg/plate |
13 |
4 |
9 |
2 |
383 |
11 |
24 |
1 |
98 |
13 |
62.5 µg/plate |
10 |
1 |
13 |
3 |
354 |
24 |
30 |
8 |
106 |
6 |
125 µg/plate |
12 |
4 |
9 |
3 |
355 |
41 |
24 |
5 |
91 |
4 |
250 µg/plate |
11 |
0 |
9 |
1 |
348 |
7 |
30 |
3 |
95 |
4 |
500 µg/plate |
4 |
3 |
6 |
3 |
344 |
26 |
14 |
2 |
22 |
39 |
Positive Control |
158 |
5 |
222 |
9 |
871 |
76 |
1710 |
75 |
1397 |
27 |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
The test substance was found to be negative in the in vitro genotoxicity Ames assay both in the presence and absence of metabolic activation. - Executive summary:
The genotoxicity of the substance was determined in a reverse mutation assay conducted in vitro with salmonella typhimurium TA 1535, TA 1537, TA 102, TA 98 and TA 100 according to the OECD Guideline 471. Concentrations tested ranged from 2.5 to 50 µg/plate and from 31.5 to 4000 µg/plate in the absence of S9 and in the presence of S9, respectively. The test substance did not induce any significant increase in the number of revertants, with or without S9 mix, in any of the 5 strains.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.