2-fluoro-6-trifluoromethylpyridine

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 305 B (Bioaccumulation: Semi-static Fish Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: Guidelines for the Testing of Chemical SEPA, P.R. China No. 305B "Bioaccumulation: Semi-static Fish Test "(May 2004).

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

Substance ID: F6TF
Lot #: CZ0904060
Purity: 99.56%

Details on sampling:

Seven fish samples and test solution samples were taken after 22, 23, 24, 25, 26, 27 and 28 days.
Each sample consists of 15 fish and about 500 mL test solution.
The concentrations of the test substance were analysed on the same day or frozen if they were not to be analysed immediately.

Sample Preparation
Water samples were filtered through 0.45 μm membranes and analysed by HPLC. Fish samples were mashed and 1.0 g were weighed and extracted with 2.0 mL of acetonitrile and water (70:30 v/v) by shaking (200 rpm, 0°C) for 10 min. After waiting for 60 min samples were centrifuged (10000 rpm, 0°C) for 10 min and filtered. The temperature was kept at 0°C during operations. The extraction solutions were filtered through 0.45 μm membranes. pH, temperature and dissolved oxygen were recorded at the same time.

Vehicle:

yes

Details on preparation of test solutions, spiked fish food or sediment:

Stock Solutions: The 200 mL stock solution (about 100.0 mg/mL) was prepared by dissolving the substance in ethanol for 7 days.

Test Solutions: The volumes of stock solution added to the 100 L dilution water were 10.0 mL and 1.0 mL for the 10 mg/L and 1 mg/L levels, respectively. After addition of the stock solution, the mixture was stirred by hand for about 5 min. In order to minimise loss of test substance due to evaporation, the test containers were equipped with a tightly fixed covers.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Individual total lengths were between 2.5 and 3.5 mm
Total wet weights between 0.2 and 0.4 g

Route of exposure:

aqueous

Test type:

semi-static

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

28 d

Hardness:

not reported

Test temperature:

23±2 ºC

pH:

6.82 - 7.56

Dissolved oxygen:

6.79 - 6.92 mg/L

Salinity:

freshwater

Details on test conditions:

Preliminary Acute Toxicity Study
Semi-static method was used to determine the LC50 for zebra fish. The LC50 was more than 100 mg/L (nominal concentration, limited test, 96 hour).

TEST SYSTEM
- Test vessel: 120 L
- Fill volume: 100 L
- Type: closed
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): 24 hours
- No. of organisms per vessel: 120
- No. of vessels per concentration (replicates): 2
- No. of vessels per control / vehicle control (replicates): 1

Nominal and measured concentrations:

Nominal concentration: 1.0, 10.0 mg/L
Mean measured concentration at Day 28: 0.976, 4.622 mg/L

Key result

Type:

BCF

Value:

< 0.06 dimensionless

Remarks on result:

other: Conc.in environment / dose:1.0 mg/L and 10.0 mg/L

Details on kinetic parameters:

Bioconcentration factors were calculated from the measured concentration in fish and test solution at steady-state using:
BCF28d = Cf/Cw
Cf is concentration in fish at day 28 (mg/kg)
Cw is concentration in water at day 28 (mg/L)

Details on results:

Preliminary Acute Toxicity Study:
Semi-static method was used to determine the LC50 for zebra fish. The LC50 was more than 100 mg/L (nominal concentration, limited test, 96 hour).

Validity of the Study and Analytical Methods:
The analytical methods were satisfactory.

Recovery of Test Substance in Fish:
The fish analytical method mean recoveries were 98.7±3.1% at 0.5 mg/kg and 74.4±6.1% at 1.26 mg/kg.

Calibration Curve:
Seven solution concentrations were used; 0.10, 0.50, 1.00, 5.04, 12.6, 25.2 and 50.4 μg/mL.
Typical linear regression: y = 12826x; R2 = 0.9997 (x= concentrations; y= peak area).
The limit of detection (LOD) of the method was 0.25 ng. The limit of quantitation (LOQ) was 0.025 mg/L in water and 0.05 mg/kg in fish.

Stability of Test Substance Solution after 24 hrs:
The exchange rate was less than 16.5% after 24 hours.

pH, Dissolved Oxygen and Temperature were maintained within acceptable ranges. Specifically, the concentration of dissolved oxygen was more than 5 mg/L during the exposure period of 28 days.

Results and Conclusions:
Neither mortality nor other adverse effects/diseases were observed in either the control or treated vessels.

Validity criteria fulfilled:

yes

Conclusions:

The BCF in zebra fish was less than 0.06. The test substance has low bioconcentration potential in zebra fish during the exposure period of 28 days.

Executive summary:

The bioconcentration test was performed with zebra fish exposed to target concentrations of 1.0 mg/L and 10.0 mg/L (nominal) with renewal of test solution every 24 hours for 28 days according to OECD Guideline 305B (Bioaccumulation: Semi-static Fish Test). No detectable concentrations of test substance were found in any sample of fish tissue. The BCF in zebra fish was less than 0.06. The test substance has low bioconcentration potential in zebra fish during the exposure period of 28 days.

 

Description of key information

 BCF < 0.06 (dimensionless)

Additional information

The bioconcentration test was performed with zebra fish exposed to target concentrations of 1.0 mg/L and 10.0 mg/L (nominal) with renewal of test solution every 24 hours for 28 days according to OECD Guideline 305B (Bioaccumulation: Semi-static Fish Test). No detectable concentrations of test substance were found in any sample of fish tissue. The BCF in zebra fish was less than 0.06. The test substance has low bioconcentration potential in zebra fish during the exposure period of 28 days.