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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1969
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: The study was conducted according to a test protocol that is comparable to the appropriate OECD test guideline. It was not compliant with GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1969
Report date:
1969

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Cyclohexanethiol
EC Number:
216-378-7
EC Name:
Cyclohexanethiol
Cas Number:
1569-69-3
Molecular formula:
C6H12S
IUPAC Name:
cyclohexanethiol
Test material form:
other: liquid
Details on test material:
- Name of test material: Cyclohexyl Mercaptan
No other details reported

Test animals / tissue source

Species:
rabbit
Strain:
New Zealand White

Test system

Vehicle:
unchanged (no vehicle)
Controls:
not required
Amount / concentration applied:
TEST MATERIAL
- Amount(s) applied: 0.1ml
- Concentration: Undiluted (100%)

Duration of treatment / exposure:
24 hours
Observation period (in vivo):
7 days
Number of animals or in vitro replicates:
5
Details on study design:
REMOVAL OF TEST SUBSTANCE
- Washing: distilled water
- Time after start of exposure: 24 hours

SCORING SYSTEM: Draize (1944), Kay and Calandra (1962)

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
cornea opacity score
Basis:
animal #1
Time point:
other: 24, 48 and 72 hours
Score:
0
Max. score:
80
Reversibility:
other: no effects
Irritation parameter:
cornea opacity score
Basis:
animal #2
Time point:
other: 24, 48 and 72 hours
Score:
0
Max. score:
80
Reversibility:
other: no effects
Irritation parameter:
cornea opacity score
Basis:
animal #3
Time point:
other: 24, 48 and 72 hours
Score:
0
Max. score:
80
Reversibility:
other: no effects
Irritation parameter:
cornea opacity score
Basis:
animal #4
Time point:
other: 24, 48 and 72 hours
Score:
0
Max. score:
80
Reversibility:
other: no effects
Irritation parameter:
cornea opacity score
Basis:
animal #5
Time point:
other: 24, 48 and 72 hours
Score:
0
Max. score:
80
Reversibility:
other: no effects
Irritation parameter:
iris score
Basis:
animal #1
Time point:
other: 24, 48 and 72 hours
Score:
0
Max. score:
10
Reversibility:
other: no effects
Irritation parameter:
iris score
Basis:
animal #2
Time point:
other: 24, 48 and 72 hours
Score:
1.67
Max. score:
10
Reversibility:
fully reversible within: 48 hours
Irritation parameter:
iris score
Basis:
animal #3
Time point:
other: 24, 48 and 72 hours
Score:
1.67
Max. score:
10
Reversibility:
fully reversible within: 48
Irritation parameter:
iris score
Basis:
animal #4
Time point:
other: 24,48 and 72 hours
Score:
1.67
Max. score:
10
Reversibility:
fully reversible within: 48 hours
Irritation parameter:
iris score
Basis:
animal #5
Time point:
other: 24, 48 and 72 hours
Score:
1.67
Max. score:
10
Reversibility:
fully reversible within: 48 hours
Irritation parameter:
conjunctivae score
Basis:
animal #1
Time point:
other: 24, 48 and 72 hours
Score:
8
Max. score:
20
Reversibility:
fully reversible within: 96 hours
Irritation parameter:
conjunctivae score
Basis:
animal #2
Time point:
other: 24, 48 and 72 hours
Score:
6.67
Max. score:
20
Reversibility:
fully reversible within: 96 hours
Irritation parameter:
conjunctivae score
Basis:
animal #3
Time point:
other: 24, 48 and 72 hours
Score:
6
Max. score:
20
Reversibility:
fully reversible within: 96 hours
Irritation parameter:
conjunctivae score
Basis:
animal #4
Time point:
other: 24, 48 and 72 hours
Score:
3.33
Max. score:
20
Reversibility:
fully reversible within: 72 hours
Irritation parameter:
conjunctivae score
Basis:
animal #5
Time point:
other: 24, 48 and 72 hours
Score:
5.33
Max. score:
20
Reversibility:
fully reversible within: 96 hours

Applicant's summary and conclusion

Interpretation of results:
slightly irritating
Remarks:
Migrated information Criteria used for interpretation of results: other: Kay and Calandra (1962)
Conclusions:
In an in vivo eye irritation study, cyclohexylmercaptan was reported to be slightly irritating according to the method of Kay and Calandra (1962), based on iritis and conjunctival redness. All effects were fully resolved by 96 hours. No corneal opacity was reported in any animal at any time point. Based on the available scores, the substance would not meet current EU criteria for classification as an eye irritant.