tetrasodium 4-{4-[(9,10-dioxo-4-{[4-(2-sulfophenoxy)phenyl]amino}-9,10-dihydroanthracen-1-yl)amino]phenoxy}benzene-1-sulfonate 4-{4-[(9,10-dioxo-4-{[4-(4-sulfophenoxy)phenyl]amino}-9,10-dihydroanthracen-1-yl)amino]phenoxy}benzene-1-sulfonate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1994

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Identification Code: FAT 21030/D
Batch No.: 16.36
Appearance: black solid
Purity: 67% AS
Solubility (in water): 80 g/L
Received : 05/11/93
Storage: room temperature
Stability: 00/12/98

Analytical monitoring:

yes

Details on sampling:

Sampling for analysis: Composite samples of ech test concentration were drawn by mixing identical volumes of the test solutions taken from the approximate center of the test vessels. They were taken imediately before exposure and after 48 hours exposure and kept at -18 °C to -22 °C until analysis.

Vehicle:

no

Details on test solutions:

Stock solution : 100.4 mg test substance were mixed and made up to 1000 ml with water.

Calculated amounts of the stock solution to produce the desired test concentrations were given into the water and were homogeneously distributed.After an equilibration time of 7 hours the daphnia were transferred into the beakers. The test substance was homogeneously distributed in the test vessels at all test concentrations at the begin of exposure.

At the end of exposure a deposit was observed at all test concentrations and small parts of the substance were swimming at the surface of the test water at concentrations 21, 46 and 100 mg/l.

Sampling for analysis : Composite samples of each test concentration were drawn by mixing identical volumes of the test solutions taken from the approximate center of the test vessels. They were taken immediately before exposure and after 48 hours exposure and kept at - 18°C to - 22°C until analysis.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain: Straus 1820
- Source: CIBA-GEIGY Ltd., testing facility
- Feeding: None during the test
- Breeding: Cultures of daphnia are maintained inglass vessels containing approx. 2.5 liter of reconstituted water (see below) at 20 ± 1 °C (water is renewed partially thrice weekly). At each renewal the daphnia are fed with a suspension of green algae (Scenedesmus subspicatus) supplemented by a suspension of TETRAMIN-extract in such quantities that the food is consumed after 24h.
- Pretreatment: 24 hours before the begin of the exposure reproductive daphnia are separated from the young by sieving all individuals through a 800 micron sieve. This operation is repeated 6 hours before the exposure and the young (6-24h of age) are retained for the test.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

Total hardness: 240 mg CaCO3 L.

Test temperature:

20 +/- 1°C

pH:

7.8-8.0

Nominal and measured concentrations:

Nominal: 4.4, 9.6, 21, 46 and 100 mg test substance/L.
Measured start: 7.0, 11.3, 22.9, 49.8 and 105.9 mg/L
Measured end: 7.0, 9.2, 11.2, 15.7 and 31.5 mg test substance/L.

Details on test conditions:

- Vessels: Beakers covered with watch glasses, 100 ml solution per beaker.
- Aeration: None
- Lighting: Fluorescent light, 16 hours daily, approx. 1500 lux.
Number of daphnia: 20 per concentration and control. 4 replicates of 5 daphnia each.
Water: Reconstituted water containing
65 mg NaHC03
294 mg CaCl2. 2 H20
123 mg MgS04.7 H20
6 mg KCl
In 1000 ml bidistilled water.The water was aerated with clean air for at least 24 hours before use.

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

11 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 32 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 32 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

11 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

Controls: Immobilizations in blank: 0 %

Reported statistics and error estimates:

None

Results of the analytical determination:

Results:

Blank (Start) 11.01.94; Content of test article in Daphnia water: <2 mg/L

Blank (End) 13.01.94; Content of test article in Daphnia water: <2 mg/L

4.4 mg/L (Start)  11.01.94; Content of test article in Daphnia water: 7 mg/L

4.4 mg/L (End) 13.01.94; Content of test article in Daphnia water: 7 mg/L

9.6 mg/L (Start)  11.01.94; Content of test article in Daphnia water: 11.3 mg/L

9.6 mg/L (End) 13.01.94; Content of test article in Daphnia water: 9.2 mg/L

21  mg/L (Start)  11.01.94; Content of test article in Daphnia water: 22.9 mg/L

21  mg/L (End)  13.01.94; Content of test article in Daphnia water: 11.2 mg/L

46 mg/L (Start)  11.01.94; Content of test article in Daphnia water: 49.8 mg/L

46 mg/L (End) 13.01.94; Content of test article in Daphnia water: 15.7 mg/L

100  mg/L (Start)  11.01.94; Content of test article in Daphnia water: 105.9 mg/L

100 mg/L (End) 13.01.94; Content of test article in Daphnia water: 31.5 mg/L

Validity criteria fulfilled:

yes

Conclusions:

The EC50 (48h) of FAT 21030/D was found to be greater than 32 mg/l.

Executive summary:

The short term toxicity potential of the test substance to the aquatic freshwater invertebrate (i.e. Daphnia magna) was studied under GLP and in accordance with EU Method C.2 (Acute Toxicity for Daphnia). The test was conducted over a period of 48 hours at the temperature of 20 °C.

The test concentrations used in this experiment were:

- Nominal: 4.4 / 9.6 / 21 / 46 and 100 mg test substance/L.

- Actual end: 7.0 / 9.2 / 11 / 16 and 32 mg test substance/L.

- Control blank: Water.

Immobilization was recorded after 24 and 48 hours exposure.

Results: EC50 (48h): >32 mg/L

Description of key information

The short term toxicity potential of the test substance to the aquatic freshwater invertebrate (i.e. Daphnia magna) was studied under GLP and in accordance with EU Method C.2 (Acute Toxicity for Daphnia). The test was conducted over a period of 48 hours at the temperature of 20 °C.

The test concentrations used in this experiment were:

- Nominal: 4.4 / 9.6 / 21 / 46 and 100 mg test substance/L.

- Actual end: 7.0 / 9.2 / 11 / 16 and 32 mg test substance/L.

- Control blank: Water.

Immobilization was recorded after 24 and 48 hours of exposure.

Results: EC50 (48h): >32 mg/L

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

32 mg/L