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EC number: 231-388-1 | CAS number: 7526-26-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1991-01-17 to 1991-03-20
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 991
- Report date:
- 1991
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Richtlinie 84/449/EWG (Amtsblatt der Europäischen Gemeinschaften Nr. L 251 vom 19.09.1984, S.103)
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Diphenyl methylphosphonate
- EC Number:
- 231-388-1
- EC Name:
- Diphenyl methylphosphonate
- Cas Number:
- 7526-26-3
- Molecular formula:
- C13H13O3P
- IUPAC Name:
- diphenyl methylphosphonate
- Details on test material:
- - Name of test material (as cited in study report): VP AC 4028
- Substance type: organic
- Physical state: solid, colourless to white
- Stability under test conditions: The performed analytical studies on the stability of the application formulations showed that VP AC 4028 was in a concentration range of about 10 to 200 mg/mL stable over the period of use.
- Storage condition of test material: dry at room temperature
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Winkelmann, Borchen (Germany)
- Age at study initiation: 10wk (males), 15 wk (females, nulliparous and non-pregnant)
- Weight at study initiation: 244g (males), 214g (females)
- Fasting period before study: no
- Housing: Makrolon cages Typ II with low-dust wood-pellet
- Diet (e.g. ad libitum): ad libitum ( "fixed-formula" Altromin® 1324 Pellets)
- Water (e.g. ad libitum):ad libitum (tape water)
- Acclimation period: 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 2 °C,
- Humidity (%):ca. 50 ± 10 %
- Air changes (per hr): 10
- Photoperiod (hrs dark / hrs light): 12/12
IN-LIFE DATES: From: To:
Administration / exposure
- Type of coverage:
- semiocclusive
- Vehicle:
- peanut oil
- Details on dermal exposure:
- TEST SITE
- Area of exposure: back
- % coverage: 10
- Type of wrap if used: aluminum foil
REMOVAL OF TEST SUBSTANCE
- Washing (if done): yes, with lukewarm water
- Time after start of exposure: 24h
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 5 mL (for dose groups 200 and 447 mg / kg/ bw).
- Concentration (if solution): not reported
- Constant volume or concentration used: yes (only for 200 and 447 mg/kg bw)
- For solids, paste formed: yes. The test substance for the dose groups 2000 and 1000 mg/kg was weighed individually for each animal according to the dose and body weight in aluminum foil, with a few drops of peanut oil DAB 9 into a paste, applied to the prepared skin area and fixed with non-irritating skin patch.
VEHICLE
- Amount(s) applied (volume or weight with unit): 5 mL
- Concentration (if solution): undilited peanut oil
- Lot/batch no.: (Fa. Lamotte, Bremen; Art.-G: 166; Charge: 5917)
- Purity: not reported - Duration of exposure:
- 24h
- Doses:
- 200, 447, 1000 and 2000 mg/kg bw
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days and 28 days
- Frequency of observations and weighing: twice daily (once on weekends and holidays). Immediately before application (day 1), after a week and at the end of the 14 to 28-day observation period the surviving animals were weighed individually.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, gross pathology. - Statistics:
- LD50 was calculated according to Spearman - Kärber (adopted from SACHS, L. (Angewandte Statistik, 6. Aufl. (1984) 178).
Results and discussion
- Preliminary study:
- no
Effect levelsopen allclose all
- Key result
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 569 mg/kg bw
- Based on:
- test mat.
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 786 mg/kg bw
- Based on:
- test mat.
- Mortality:
- No mortalities occured in both sexes in the dose group of 200 mg/kg bw. No mortalities occured in males in the dose group of 447 mg/kg bw. One female animal in the 447 mg/kg bw dose group was found dead at the 5th day of postapplication period. The mortalities in the 1000 and in the 2000 mg/kg bw occured in the first week of observation period.
- Clinical signs:
- other: Single administration of 1000 and 2000 mg/kg bw of test material produced systemic poisoning symptoms: poor general condition, ruffled fur, chromodakryorrhoe, tremors, snap or labored breathing, apathy and loss of weight. In females of the dose group 447
- Gross pathology:
- No gross pathologic findings were noted in treated animals. In some animals the eye rims and nose were bloody, which is in accordance with the clinically observed Chromodakryorrhoe.
- Other findings:
- no
Any other information on results incl. tables
Table 1. Mortalities
Dose (mg/kg bw) |
No. of animals |
Time of death |
Mortality (%) |
Males |
|||
200 |
0/5 |
- |
0 |
447 |
0/5 |
- |
0 |
1000 |
4/5 |
2d-7d |
80 |
2000 |
3/5 |
2d-3d |
60 |
Females |
|||
200 |
0/5 |
- |
0 |
447 |
1/5 |
5d |
20 |
1000 |
5/5 |
1d-3d |
100 |
2000 |
4/5 |
2h-4d |
80 |
Table 2. Body weights
Dose |
Males (g)^ |
Females (g)^ |
||||||||||||
|
Day 1 |
Day 8 |
Day 15 |
Day 1 |
Day 8 |
Day 15 |
||||||||
200 |
264 |
284 |
304 |
221 |
226 |
240 |
||||||||
447 |
227 |
245 |
276 |
215 |
216° |
222° |
||||||||
1000 |
252 |
227* |
248* |
217* |
-- |
-- |
||||||||
|
Day 1 |
Day 8 |
Day 15 |
Day 21 |
Day 28 |
Day 1 |
Day 8 |
Day 15 |
Day 21 |
Day 28 |
||||
2000 |
234 |
168** |
194** |
223** |
248** |
202 |
156* |
182* |
199* |
207* |
^Mean
of bodyweights
°based on 4 animals
*based on 1 animal
**based on 2 animals
-- Dead animals
Table 3. Clinical signs
Dose |
200 |
447 |
1000 |
2000 |
Males |
||||
Number of dead animals |
0 |
0 |
4 |
3 |
Number of animals with symptoms |
0 |
0 |
5 |
5 |
Number of treated animals |
5 |
5 |
5 |
5 |
Symptoms from day |
-- |
-- |
2 |
4 |
Symptom-free from day |
-- |
-- |
13 |
--0 |
|
Number of animals with symptoms/number of animals with symptoms of the highest intensity |
|||
Ruffled fur |
0 |
0 |
5/3 |
1/3 |
Poor general condition |
0 |
0 |
2/3 |
5/3 |
Tremors |
0 |
0 |
3/3 |
5/1 |
Chromodakryorrhoe |
0 |
0 |
5/* |
5/* |
Snap breathing |
0 |
0 |
5/* |
0 |
Apathy |
0 |
0 |
5/3 |
0 |
Females |
||||
Number of dead animals |
0 |
1 |
5 |
4 |
Number of animals with symptoms |
0 |
3 |
4 |
5 |
Number of treated animals |
5 |
5 |
5 |
5 |
Symptoms from day |
-- |
2 |
2 |
2h |
Symptom-free from day |
|
6 |
3 |
14 |
|
Number of animals with symptoms/number of animals with symptoms of the highest intensity |
|||
Ruffled fur |
0 |
0 |
4/3 |
1/1 |
Poor general condition |
0 |
3/3 |
1/3 |
4/3 |
Tremors |
0 |
3/2 |
2/3 |
5/2 |
Chromodakryorrhoe |
0 |
0 |
3/* |
4/* |
Snap breathing |
0 |
1/* |
3/* |
1/1 |
Apathy |
0 |
0 |
4/3 |
0 |
Eyes whitish discoloured |
0 |
0 |
1/* |
0 |
Applicant's summary and conclusion
- Interpretation of results:
- Category 3 based on GHS criteria
- Remarks:
- based on EU GHS
- Conclusions:
- Diphenyl methylphosphonate is considered to possess toxicity via dermal route of exposure and is classified as Cat 3 (H311 toxic in contac with skin) due to LD50 of 569 mg/kg bw for females and LD50 of 786 mg/kg bw for males.
- Executive summary:
The objective of this study was to determine the acute dermal LD50 of diphenyl methylphosphonate applied to the skin of male and female Wistar rats. 5 animals of each sex were dosed with 200, 447, 1000 and 2000 mg/kg bw. The test material remained in contact with the skin for 24 hours. All surviving animals were observed closely for gross signs of systemic toxicity and mortality at frequent intervals during the day of dosing and at least twice daily thereafter for a total of 14 days (animals in the dose group of 2000 mg/kg bw were observed during 28 days). Immediately before application (day 1), after a week and at the end of 14- or 28 -day observation period the surviving animals were weighed individually. A necropsy was conducted on all surviving animals at the end of observation period.
Males tolerated 200 and 447 mg/kg bw without symptoms, while females tolerated only 200 mg/kg bw. Clinical signs of sytemic toxicity were poor general condition, ruffled fur, chromodakryorrhoe, tremors and difficulties in breathing (or snaping). general condition of the surviving animals in the highest dose group was normalized till the 28 -day of observation. No local skin reactions were noted in animals at the test site of application. Body weights of animals in the 1000 and 2000 mg/kg bw decrased significantly. The growth of males treated with 200 and 447 mg/kg bw was not affected. The body weight of three females in the dose group of 447 mg/kg bw decreased marginally in the first week of observation period. Body weights increased clearly in all surviving animals at the end of the observation period. No gross pathological findings were noted in treated animals. In some animals the eye rims and nose were bloody, which is in accordance with the clinically observed chromodakryorrhoe.
The calculated LD50 values were 786 mg/kg bw and 569 mg/kg bw for males and females, respectively. Based on these LD50 values, the classification is warranted according to the criteria of EU Classification, Labelling and Packaging of Substances and Mixtures (CLP) Regulations No 1272/2008:
Acute dermal toxicity: Cat3 (H311 - toxic in contact with skin)
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