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EC number: 231-388-1 | CAS number: 7526-26-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 05 SEP 1979 - 01 JUN 1980
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: well documented old study, which meets basic scientific principles
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 980
- Report date:
- 1980
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- GLP compliance:
- not specified
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Diphenyl methylphosphonate
- EC Number:
- 231-388-1
- EC Name:
- Diphenyl methylphosphonate
- Cas Number:
- 7526-26-3
- Molecular formula:
- C13H13O3P
- IUPAC Name:
- diphenyl methylphosphonate
- Details on test material:
- - Name of test material (as cited in study report): methanphosphonsäurediphenylester
- Substance type: organic
- Physical state: liquid
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Winkelmann, Borchen
- Age at study initiation: approximately 9 weeks
- Weight at study initiation: ca. 190 gr
- Fasting period before study:
- Housing: groups of five, conventionally in Makrolon cages on dust-free wood granules
- Diet (e.g. ad libitum): Altromin R1324 (Altromin GmbH, Lage), ad libitum
- Water (e.g. ad libitum): drinking water ad libitum
- Acclimation period:
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 1.5 °C
- Humidity (%): 60 +/- 5 %
- Air changes (per hr):
- Photoperiod (hrs dark / hrs light): 12 h light/ 12 hours dark
OTHER: identification of the animals was secured by marking of the fur with picrinic acid and cage-labels
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- unchanged (no vehicle)
- Details on oral exposure:
- MAXIMUM DOSE VOLUME APPLIED: 0.4 mL / kg bw
- Doses:
- 0.05, 0.1, 0.2, 0.3, 0.35 and 0.4 mL/kg bw
- No. of animals per sex per dose:
- 10
- Control animals:
- not specified
- Details on study design:
- The test item was administered with the aid of a rigid oesophagus-tube to 10 animals.
The animals were inspected repeatedly on the day of administration, thereafter the animals were inspected twice daily (once daily on weekend days and on holidays) for 14 days and the character, onset, duration and intensity of the clinical symptoms was recorded. Whenever dead animals were found they were removed.
Bodyweights were determined individually shortly before adminsitration and after the end of the observation period. - Statistics:
- The LD50 value was calculated (p < 0.05) according to the programmed Probit-analysis (Fink and HUnd (Arzneimittelforschung 15, 1965, p. 624).
Results and discussion
Effect levelsopen allclose all
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 0.19 mL/kg bw
- Based on:
- test mat.
- 95% CL:
- >= 0.14 - <= 0.25
- Key result
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 233 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: conversion of the above mentioned value, based on density available for the test item
- Mortality:
- no animal died in the dose group, which received 0.05 mL/kg bw. 3 animals died in the dose group of 0.1 mL/kg bw. 4 and 6 animals died in the dose groups 0.2 and 0.3, respectively. 8 animals died, dosed with 0.35 mL/kg bw and all animals died in the highest dose group (0.4 mL/kg bw).
- Clinical signs:
- other: The signs of intoxication were ruffled fur, sedation, bloody eyes and nose, these clinical signs were to be noted in all dose groups beside 0.05 mL/kg bw.
Any other information on results incl. tables
The
single doses of
0.1
- 0.4ml/
kg bw had
the following symptoms of poisoning in
all animals: shaggy
fur,sedation,
bloody eyesand
nose.
At
starting
doses of
0.3 to
0.35mL/
kg bw weight
loss was
also observed.
The
symptoms
occurred within 30
minutes after
application
and were
slightly
pronounced
and
lasted until
the last day of the observation period.
The
deaths were
reported from
day 1to
7.
The
calculated LD50
value was
0.19 ml/
kgbw (confidence
interval
for p<0.05
=0:14
to 0:25mL
kgbw).
When
increasing
the probit
regression
line(probit-slope-factor)
resulted
in a value
of b=3.36.
The
dose of
0.05 mL/
kgbw was
tolerated
without
symptoms.
Applicant's summary and conclusion
- Interpretation of results:
- Category 3 based on GHS criteria
- Remarks:
- based on EU GHS
- Conclusions:
- The study was performed equivalent of similar to the OECD TG401 and therefore considered to be of high quality (reliability Klimisch 2). The test material did induce mortality and treatment-related clinical signs in the dose groups of 0.1 - 0.4 mL/kg bw (100, 80 %, 60 & 40 % and 30 % mortality). The test material was considered to be toxic if swallowed after oral application under the conditions of the test and as the LD50 of the test item is 50 - 300 is has to classified as GHS Category 3.
- Executive summary:
The acute oral toxicity of diphenyl methylphosphonate (MPS) was investigated in rats (Löser, 1980). The study was performed equivalent or similar to the OECD Guideline 401 and considered to be of high quality (reliability Klimisch 2). 10 male rats per group received doses of 0.05 mL/kg bw, 0.1 mL/kg bw, 0.2 mL/kg bw, 0.3 mL/kg bw, 0.35 mL/kg bw or 0.4 mL/kg bw via a rigid tube orally. The symptoms of intoxications were ruffled hair coat, sedation, bloody eyes and nose and in addition body weight loss (0.3 and 0.35 mL/kg bw). All animals in the highest dose group died within the observation period. In the 0.35 and the 0.3mL/kg bw dose group 8 and 6 animals died, respectively. In the 0.2 and 0.1 mL/kg bw dose group 4 and 2 animals died, respectively. No animals died in the lowest dose group. No clinical symptoms were noted in the lowest dose group. Therefore the oral toxicity can be characterised by a LD50 value of 233 mg/kg bw.
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