Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 477-690-9 | CAS number: 874819-71-3
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2004-09-09 - 2004-10-27
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The GLP study was conducted according to an internationally accepted guideline. All study parameters are based on the specific guideline.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 004
- Report date:
- 2004
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Version / remarks:
- OECD 405 (2002)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- -
- EC Number:
- 477-690-9
- EC Name:
- -
- Cas Number:
- 874819-71-3
- Molecular formula:
- Hill formula: C6H9N4O3P CAS formula: C6H9N4O3P
- IUPAC Name:
- N-(diaminophosphoryl)-2-nitroaniline
- Test material form:
- solid: crystalline
- Details on test material:
- Nature of substance: solid, grinded in a mortar
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- Species/strain: Rabbits, breed: White New Zealanders (SPF Crl:NZW)
Sex: male
Supplier: Charles River Wiga GmbH, D-97320 Sulzfeld
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- not required
- Amount / concentration applied:
- 0.1 ml
- Duration of treatment / exposure:
- 1 h
- Observation period (in vivo):
- 72 h
- Number of animals or in vitro replicates:
- 3
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- not specified
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- iris score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 2
- Reversibility:
- not specified
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- conjunctivae score
- Remarks:
- redness
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 3
- Reversibility:
- not specified
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- conjunctivae score
- Remarks:
- chemosis
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- not specified
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- chemosis score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- not specified
- Remarks on result:
- no indication of irritation
- Other effects:
- Only a slight redness of the conjunctivae in all animals one hour after instillation. One day after instillation signs of irritations were not more observed.
Any other information on results incl. tables
Alteration |
Ani |
Observed grades of ocular lesions at each observation time |
|||||||
|
mal |
Hours after instillation |
|||||||
|
No. |
1 |
24 |
48 |
72 |
||||
|
|
Control |
Test item |
Control |
Test item |
Control |
Test item |
Control |
Test item |
Cornea |
1 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
2 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
3 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Iris |
1 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
2 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
3 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Conjunctivae |
1 |
0 |
1 |
0 |
0 |
0 |
0 |
0 |
0 |
Redness |
2 |
0 |
1 |
0 |
0 |
0 |
0 |
0 |
0 |
|
3 |
0 |
1 |
0 |
0 |
0 |
0 |
0 |
0 |
Conjunctivae |
1 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Chemosis |
2 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
3 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Applicant's summary and conclusion
- Interpretation of results:
- not irritating
- Remarks:
- Migrated information not classified Criteria used for interpretation of results: EU
- Conclusions:
- N-(2-Nitrophenyl)phosphoric triamide is non-irritant to the eye.
- Executive summary:
The acutc eye irritation/corrosion of N-(2-NitrophenyI)phosphoric triamide was tested in three albino rabbits according to OECD guideline 405. The test item was instilled as the original substance after crushing with a pestle and mortar to a fine dust at a single dose of 0.1 ml to one of the eyes in each animal. The untreated eye was used as control.
The animals were examined for clinical signs and the eyes were examined for lesions of the conjunctivae, cornea and iris 60 minutes, 24, 48 and 72 hours after instillation of the test item. The grades for ocular lesions were recorded in accordance with the OECD guideline.
The instillation of the test item caused only a slight redness of the conjunctivae in all animals one hour after instillation.
The cornea and the iris were not affected.
One day after instillation signs of irritations were not more observed. None of the animals died or showed clinical signs during the course of testing.
The alterations of the eyes after instillation of N-(2-Nitrophenyl)phosphoric triamidedo not meet the criteria for classification of a substance as an eye irritant.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
