Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes
Test organisms (species):
Daphnia magna
Test type:
static
Water media type:
freshwater
Total exposure duration:
48 h
Hardness:
CaCl2 x 2 H2O: 294 mg/l, MgSO4 x 7 H2O: 123 mg/l
Test temperature:
20 +- 2°C
pH:
7.0 to 7.5
Dissolved oxygen:
6.6 to 7.8 mg/l
Salinity:
- KCl: 5.5 mg/l
- Ca/Mg ratio: 4 : 1
- Na/K ratio: 10 : 1
Nominal and measured concentrations:
100, 140, 200, 280, 400, 560, 800 mg
Details on test conditions:
TEST ORGANISMS
- Strain: Daphnia magna Straus Clone 5
- Source/supplier: Hüls AG
- Breeding method: Breeding method according to Elendt (1990) in M4-medium in 1l beakers, water exchange every 2 to 3 days.
- Age: < 1 day
- Feeding: Desmodesmus subspicatus
- Feeding during test: none
- Control group: negative control (water only), positive control: potassium dichromate
STOCK AND TEST SOLUTION AND THEIR PREPARATION
- Vehicle, solvent: water
- Concentration of vehicle/ solvent: 1000 mg/l
STABILITY OF THE TEST CHEMICAL SOLUTIONS: see stability information (hydrolysis as function of pH).
DILUTION WATER
- Source: Synthetic fresh water
- Aeration: no
- Hardness: CaCl2 x 2 H2O: 294 mg/l, MgSO4 x 7 H2O: 123 mg/l
- Salinity: KCl: 5.5 mg/l
- Ca/Mg ratio: 4 : 1
- Na/K ratio: 10 : 1
- pH: 7.0 to 7.5
- Oxygen content: 6.6 to 7.8 mg/l
TEST SYSTEM
- Test type: static
- Concentrations: (nominal): 100, 140, 200, 280, 400, 560, 800 mg/l
- Exposure vessel type: round bottom flasks
- Number of replicates, individuals per replicate: 4 replicates, 5 individuals
- Test temperature: 20 +- 2°C
- Dissolved oxygen: 6.6 - 7.8 mg/l
- pH: 7.0 - 7.5
- Adjustment of pH: no
- Intensity of irradiation: dark
DURATION OF THE TEST: 48 hours
TEST PARAMETER: Immobilization
MONITORING OF TEST SUBSTANCE CONCENTRATION: not performed, nominal concentrations used.
Statistical Analyis: Probit analysis according to Cavalli-Sforza
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
202.3 mg/L
Nominal / measured:
nominal
Remarks on result:
other: 175.2-233.6 mg/L
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
400 mg/L
Nominal / measured:
nominal
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
285.8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks on result:
other: 244.6-333.9 mg/L
Details on results:
Based on effective concentrations (corrected for hydrolysis) the EC50 48 h is 179 mg/l.
Validity criteria fulfilled:
not specified
Conclusions:
Based on effective concentrations (corrected for hydrolysis) the EC50 48 h is 179 mg/l.

Description of key information

Based on effective concentrations (corrected for hydrolysis) the EC50 48 h is 179 mg/l.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
179 mg/L

Additional information