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EC number: 203-305-9 | CAS number: 105-53-3
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 07/06/1981 - 07/10/1981
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Materials and methods are well described. Thus the study can be considered as reliable.
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- concentration of stock solution: 15.8 g/L
- Vehicle:
- no
- Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- age: 33 days
mean length: 22.0 mm
SD length: 2.708 mm
mean weight: 0.152 g
SD weight: 0.0703 g
loading: 0.603 g/L - Water media type:
- freshwater
- Total exposure duration:
- 96 h
- Hardness:
- 45.0 mg CaCO3/L
- Test temperature:
- 25.4 °C
- pH:
- 7.42
- Dissolved oxygen:
- 7.5 mg/L
- Details on test conditions:
- Tank volume: 6.3 L
Alkalinity: 44.0 mg CaCO3/L
25 animals per tank
2 tests for each concentration - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 15.4 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 15.2 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- behaviour
- Remarks:
- general effects
- Sublethal observations / clinical signs:
mortalities reported (25 animals per tank):
hours control A B C D E 3 0 / 0 0 / 0 0 / 0 0 / 0 0 / 0 0 / 0 5 0 / 0 0 / 0 0 / 0 0 / 0 0 / 0 4 / 5 20 0 / 0 0 / 0 0 / 0 4 / 5 19 / 21 24 / 25 24 0 / 0 0 / 0 0 / 0 4 / 6 20 / 23 25 / 25 44 0 / 0 0 / 0 1 / 1 11 / 16 25 / 25 25 / 25 48 0 / 0 0 / 0 1 / 1 12 / 16 25 / 25 25 / 25 72 0 / 0 0 / 0 1 / 1 16 / 17 25 / 25 25 / 25 96 0 / 0 0 / 0 1 / 1 19 / 17 25 / 25 25 / 25 LC50, 96 h: 15.4 mg/L
conf. lim.: 14.1 - 16.9 mg/L
effect reported (25 animals per tank):
hours control A B C D E 3 0 / 0 0 / 0 0 / 0 0 / 0 0 / 0 2 / 2 5 0 / 0 0 / 0 0 / 0 0 / 0 0 / 1 14 / 14 20 0 / 0 0 / 0 0 / 0 4 / 9 20 / 21 24 / 25 24 0 / 0 0 / 0 0 / 0 7 / 9 22 / 24 25 / 25 44 0 / 0 0 / 0 1 / 1 15 / 17 25 / 25 25 / 25 48 0 / 0 0 / 0 1 / 1 15 / 18 25 / 25 25 / 25 72 0 / 0 0 / 0 1 / 1 16 / 18 25 / 25 25 / 25 96 0 / 0 0 / 0 1 / 1 19 / 18 25 / 25 25 / 25 EC50, 96 h: 15.2 mg/L
conf. lim.: 14.0 - 16.6 mg/L
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the test results a LC50, 96 h of 15.4 mg/L with confidential limits of 14.1 - 16.9 mg/L can be stated.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 05/09/1983 - 05/13/1983
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Materials and methods are well described. Thus the study can be considered as reliable.
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- concentration of stock solution: 260 mg/L
- Vehicle:
- no
- Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- age: 28 days
mean length: 19.8 mm
SD length: 2.149 mm
mean weight: 0.120 g
SD weight: 0.0324 g
loading: 1.200 g/L - Water media type:
- freshwater
- Total exposure duration:
- 96 h
- Hardness:
- 37.4 mg CaCO3/L
- Test temperature:
- 25.1 °C
- pH:
- 7.68
- Dissolved oxygen:
- 7.1 mg/L
- Details on test conditions:
- Tank volume: 2.0 L
Alkalinity: 38.6 mg CaCO3/L
20 animals per tank
1 test for each concentration - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 17.4 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 16.8 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- not specified
- Remarks:
- general effects
- Sublethal observations / clinical signs:
mortalities reported (20 animals per tank):
hours control A B C D E 20 0 0 0 1 12 20 24 0 0 0 2 14 20 44 0 0 0 3 16 20 48 0 0 0 7 16 20 72 0 0 1 12 17 20 92 0 0 4 14 19 20 96 0 0 5 14 20 20 LC50, 96 h: 17.4 mg/L
conf. lim.: 15.4 - 19.5 mg/L
effect reported (20 animals per tank):
hours control A B C D E 20 0 0 0 3 16 20 24 0 0 0 6 16 20 44 0 0 0 11 17 20 48 0 0 1 12 16 20 72 0 0 1 14 19 20 92 0 0 5 14 20 20 96 0 0 7 14 20 20 EC50, 96 h: 16.8 mg/L
conf. lim.: 14.9 - 18.9 mg/L
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the test results a LC50, 96 h of 17.4 mg/L with confidential limits of 15.4 - 19.5 mg/L can be stated.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 05/21/1985 - 05/25/1985
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Materials and methods are well described. Thus the study can be considered as reliable.
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- concentration of stock solution: 500 mg/L
- Vehicle:
- no
- Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- age: 28 days
mean length: 22.0 mm
SD length: 1.432 mm
mean weight: 0.165 g
SD weight: 0.0338 g
loading: 2.750 g/L - Water media type:
- freshwater
- Total exposure duration:
- 96 h
- Hardness:
- 53.0 mg CaCO3/L
- Test temperature:
- 23.9 °C
- pH:
- 7.27
- Dissolved oxygen:
- 7.0 mg/L
- Details on test conditions:
- Tank volume: 1.2 L
Alkalinity: 41.1 mg CaCO3/L
20 animals per tank
1 test for each concentration - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 11.8 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 11.1 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- not specified
- Remarks:
- general effects
- Sublethal observations / clinical signs:
mortalities reported (20 animals per tank):
hours control A B C D E 24 0 0 0 0 9 18 48 0 0 0 12 19 20 72 0 0 4 17 19 20 96 0 2 5 17 20 20 LC50, 96 h: 11.8 mg/L
conf. lim.: 10.3 - 13.4 mg/L
effect reported (20 animals per tank):
hours control A B C D E 24 0 0 0 3 15 20 48 0 0 1 17 19 20 72 0 1 4 17 19 20 96 0 2 7 17 20 20 EC50, 96 h: 11.1 mg/L
conf. lim.: 9.65 - 12.8 mg/L
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the test results a LC50, 96 h of 11.8 mg/L with confidential limits of 10.3 - 13.4 mg/L can be stated.
Referenceopen allclose all
Description of key information
Three independent short-term toxicity tests were conducted with diethyl malonate on pimephales promelas.
The LC50, 96 h values determined by the studies were in the range of 11.8 - 17.4 mg/L.
Thus the LC50 for fish for diethylmalonate can be stated as >= 11.8 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 11.8 mg/L
Additional information
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Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
