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EC number: 421-090-1 | CAS number: 131298-44-7 Isodecyl benzoate
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1996
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- An in vitro gene mutation study in bacteria is a standard information requirement in Annex VII to REACH. The study meets the requirements of OECD TG 471 (1996) because it was performed with 5 strains: four strains of S. typhimurium (TA98; TA100; TA1535; TA1537) and another strain that is E. coli WP2 uvrA.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- -
- EC Number:
- 421-090-1
- EC Name:
- -
- Cas Number:
- 131298-44-7
- Molecular formula:
- C17H26O2
- IUPAC Name:
- undecyl benzoate
- Reference substance name:
- Benzoic acid, C9-11, C10-rich branched alkyl esters
- IUPAC Name:
- Benzoic acid, C9-11, C10-rich branched alkyl esters
- Reference substance name:
- Isodecyl benzoate
- IUPAC Name:
- Isodecyl benzoate
Constituent 1
Constituent 2
Constituent 3
Method
Species / strainopen allclose all
- Species / strain / cell type:
- E. coli WP2 uvr A
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254 induced rat liver preparations
- Test concentrations with justification for top dose:
- 0, 312.5, 625, 1250, 2500, 5000 ug/ plate. No toxicity observed at 5000 ug/plate
- Vehicle / solvent:
- DMSO
Controlsopen allclose all
- Positive controls:
- yes
- Positive control substance:
- other: In presence of S-9 mix: 2-Aminoanthracene
- Remarks:
- 2ug/plate (TA 1535/1537), 0.5 ug/plate (TA 98), 1ug/plate (TA 100), 10ug/plate (WP2 uvrA)
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- Remarks:
- Absence of S-9 mix - 1 ug/plate for strain TA 98
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- Absence of S-9 mix - 80 ug/plate for TA 1537
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Remarks:
- N-ethyl-N-nitro-N-nitroguanidine 5ug/plate (TA 1535), 3ug/plate (TA 100) , 2 ug/plate (WP2 uvrA)
- Positive control substance:
- N-ethyl-N-nitro-N-nitrosoguanidine
- Remarks:
- Absence of S-9 mix - N-ethyl-N-nitro-N-nitroguanidine 5ug/plate (TA 1535), 3ug/plate (TA 100) , 2 ug/plate (WP2 uvrA)
- Details on test system and experimental conditions:
- PRELIMINARY TOXICITY TEST
Four concentrations of test substance were assessed for toxicity using the five tester strains. The
highest concentration was 50 mg/mL of test substance in the hosen solvent, which provided final
concentration of 5000 ug/plate. Three 10-fold serial dilutions of the highest concentration were also
tested. The chosen sołvent, dimethyl sulphoxide, was used as the negative control.
An aliquot of 0.I ml of a 10 hour bacterial culture and 0.5 ml S-9 nix or 0.5 mi 0.1 M phosphate
buffer (pH 7.4) were placed in glass bottles. An aliquot of 0.1 ml of the test solation was added,
followed immediately by 2 ml of histidine/tryptophan deficient agar. The mixture was thoroughly
shaken and overlaid onto previously prepared petri dishes containing 25 mi minimal agar. A single
petri dish was used for each dose level. Plates were also prepared without the addition of bacteria
in order to assess the sterility of the test substance, S-9 mix and phosphate buffer, All plates were
incubated at 37"C for 3 days. After this period the appearance of the background bacterial lawn was
examined. Revertant colonies were counted using a Seescan Automatic Colony Counter
Any toxic effects of the test substance can be detected by a substantial reduction in revertant colony
counts or by the absence of a complete background bacterial lawn. In the absence of any toxic effects
the top concentration used in the main tests is the same as that used in the preliminary toxicity test.
If toxic effects are observed a lower concentration may be chosen for the main assays. ideally the
concentrations chosen for the mutation tests should include a minimum of four non-toxic
concentrations.
MUTATION TEST PROCEDURE
The test substance was added to cultures of the five tester strains at five concentrations separated by
2-4 fold dilutions. The highest concentration of isodecyl benzoate used was S000 plate. The negative control was the chosen solvent, dimethyl sulphoxide The positive control compounds were
also included.
An aliquot of 0. ml of a 10 hour bacterial culture and 0.5 mi S9 mix or 0.5 ml 0,.1 M phosphate
buffer (pH 7.4) were placed in glass bottles, An aliquot of 0.I al of the test solution wis added,
followed immediately by 2 ml of histidine/tryptophan deficient agar. The mixture was thoroughly
shaken and overlaid onto previously prepared petri dishes containing 25 ml minimal agar, Three petri
dishes were used for each dose level. A set of plates were also prepared containing only bacterial
culture and S9 mix or phosphate buffer (0 ug/plate). Plates were also prepared without the addition
of bacteria in order to assess the sterility of the test substance, S-9 mix and phosphate buffer, All
plates were incubated at 37C for 3 days. After this period revertant colonies were counted using
a Seescan Automatic Colony Counter.
At a later date the main test was repeated using the procedure described above with the same
concentrations of test substance
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- not specified
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- not specified
- Positive controls validity:
- valid
- Key result
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- not specified
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- not specified
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- not specified
- Positive controls validity:
- valid
Applicant's summary and conclusion
- Conclusions:
- Isodecyl benzoate is not mutagenic.
- Executive summary:
The revertant colony counts for isodecyl benzoate obtained in the preliminary toxicity test are showed
chosen as the top dose level in the mutation tests. Isodecyl benzoate was not toxic towards the tested strains. Therefore 5000 ug/plate was chosen as the top dose level in the mutation tests.
No substantial increases in revertant colony numbers of any of the tester strains were observed
following treatment with isodecyl bezoate at any dose level, in the presence or abscence of S-9 mix,
in either mutation test.
The concurrent positive control compounds demonstrated the sensitivity of the say and he
metabolizing activity of the liver preparations.
CONCLUSION
It is concluded that, when tested in dimethyl sulphoxide, Isodecyl benzoate shows no evidence of
mutagenic activity in these bacterial systems.
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