Registration Dossier
Registration Dossier
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EC number: 201-126-0 | CAS number: 78-59-1
Specific investigations: other studies
Administrative data
- Endpoint:
- biochemical or cellular interactions
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Adrenal-dependent leucopenia after short-term exposure to various airborne irritants in rats
- Author:
- Brondeau MT, Bonnet P, Guenier JP, Simon P, de Ceaurriz J
- Year:
- 1 990
- Bibliographic source:
- J Appl Toxicol 10(2): 83-86
Materials and methods
Test guideline
- Qualifier:
- no guideline required
- GLP compliance:
- not specified
- Type of method:
- in vivo
- Endpoint addressed:
- other: blood cell count
Test material
- Reference substance name:
- 3,5,5-trimethylcyclohex-2-enone
- EC Number:
- 201-126-0
- EC Name:
- 3,5,5-trimethylcyclohex-2-enone
- Cas Number:
- 78-59-1
- Molecular formula:
- C9H14O
- IUPAC Name:
- 3,5,5-trimethylcyclohex-2-enone
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: 250-300 g
- Diet: ad libitum, except during exposure
- Water: ad libitum, except during exposure
- Acclimation period: at least 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22
- Photoperiod (hrs dark / hrs light): 12 / 12
Administration / exposure
- Route of administration:
- inhalation: vapour
- Vehicle:
- unchanged (no vehicle)
- Analytical verification of doses or concentrations:
- yes
- Duration of treatment / exposure:
- 4 hours
- Frequency of treatment:
- once
Doses / concentrationsopen allclose all
- Dose / conc.:
- 19 ppm
- Remarks:
- corresponds to 0.107 mg/L
- Dose / conc.:
- 49 ppm
- Remarks:
- corresponds to 0.276 mg/L
- Dose / conc.:
- 67 ppm
- Remarks:
- corresponds to 0.378 mg/L
- Dose / conc.:
- 90 ppm
- Remarks:
- corresponds to 0.508 mg/L
- No. of animals per sex per dose:
- 5
- Control animals:
- not specified
- Details on study design:
- At the end of the exposure period, rats were anaesthetized with pentobarbital and exsanguinated from the abdominal aorta.
Examinations
- Examinations:
- Blood samples were collected in tubes containing complexon as an anticoagulant.
Red blood cells, total white blood cell and leukocyte differential counts were performed.
Results and discussion
- Details on results:
- A statistically significant decrease in the number of circulating leukocytes (without any change in differential or red blood cell counts) was observed in rats exposed for 4 hours to increasing concentrations of the test substance.
Applicant's summary and conclusion
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