L-arginine phosphate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1991-09-30 to 1992-05-20

Reliability:

2 (reliable with restrictions)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Arginine

EC Number:

200-811-1

EC Name:

Arginine

Cas Number:

74-79-3

Molecular formula:

C6H14N4O2

IUPAC Name:

arginine

Test material form:

not specified

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test substance was weighed out and each portion was dissolved in 1 l of dilution
water. 10 g were dissolved in 1 l for the initial limit test as well as for the full test. For the latter test aliquots were diluted to 500 ml dilution water.
- Eluate: The dilution water used was DSWL, prepared from groundwater.
- Controls: DSWL (negative control)
- Evidence of undissolved material (e.g. precipitate, surface film, etc): visually

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: daphnia, water flea
- Source: TNO
- Age at study initiation (mean and range, SD): < 24 h
- Method of breeding: according to principles of NPR 6503 - Feeding during test: no

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Test conditions

Hardness:

Hardness, expressed as CaCO3: 204 mg/l.

Test temperature:

20°C +/- 1°C

pH:

All data are for the full test.
Control medium: Beginning of test: 7.9. End of test: 7.7
1 g/l test substance: Beginning of test: 9.4. End of test: 8.9
1.8 g/l test substance: Beginning of test: 9.7. End of test: 9.0
3.2 g/l test substance: Beginning of test: 9.9. End of test: 9.1 to 9.2 5.6 g/l test substance: Beginning of test: 10.0. End of test: 9.3 to 9.4 10 g/l test substance: Beginning of test: 10.3. End of test: 9.6

Dissolved oxygen:

All data are for the full test.
Control medium: Beginning of test: 8.5 mg/l. End of test: 7.6 to 8.1 mg/l.
1 g/l test substance: Beginning of test: 8.8 mg/l. End of test: 8.2 mg/l.
1.8 g/l test substance: Beginning of test: 8.7 mg/l. End of test: 8.3 to 8.4 mg/l. 3.2 g/l test substance: Beginning of test: 8.9 mg/l. End of test: 8.4 to 8.5 mg/l. 5.6 g/l test substance: Beginning of test: 8.7 mg/l. End of test: 8.3 to 8.5 mg/l. 10 g/l test substance: Beginning of test: 8.7 mg/l. End of test: 8.4 to 8.5 mg/l

Salinity:

Not applicable

Nominal and measured concentrations:

Initial limit test: Nominal concentration of test substance: 10 g/l
Full test: Nominal concentrations of test substance: 1.0, 1.8, 3.2, 5.6, 10 g/l

Details on test conditions:

TEST SYSTEM
- Test vessel: all-glass beakers
- Type: open
- Material, size, fill volume: glass, 150 ml
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): no renewal
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Groundwater from Linschoten, the Netherlands. Medium prepared by adding several salts.
- Total organic carbon: 1.5 mg/l
- Metals: << 1 mg/l
- Chlorine: 2.71 mml/l
- Na+: 1.19 mmol/l
- K+: 0.22 mmol/l
- Ca 2+: 1.30 mmol/l
- Mg2+. 0.74 mmol/l
- SO42-: 0.69 mmol/l
- Ca/mg ratio: 1.76
- Intervals of water quality measurement: Once prior to beginning for the above parameters

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light - 8 h dark, with transition periods of 30 minutes

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : EC50, EC100, NOEC (mobility = 24h EC0), NOEC (condition) after 24 h.
Condition: Swimming behaviour, colour, or any other morphological or visual behavioural criterion after 24 h.

TEST CONCENTRATIONS
- Test concentrations: 1.0, 1.8, 3.2, 5.6, 10 mg/l

TEST CONCENTRATION INITIAL LIMIT TEST
- Range finding study: yes
- Test concentrations: 10 mg/l
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 8
- No. of vessels per control (replicates): 8
- Results used to determine the conditions for the definitive study: lower concentrations and concentration of limit test

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

1.8 g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% confidence interval: 1.5 - 2.1 g/l

Effect concentrations 2

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

5.6 g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

24 h

Dose descriptor:

NOEC

Remarks:

(mobility)

Effect conc.:

1 g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks:

(= 24h EC0)

Remarks on result:

other: The NOEC values were estimated by comparing effects with regard to mobility, according to the definition given in OECD 202 (ref. 1) and condition of the exposed animals with those of the control animals.

Effect concentrations 4

Duration:

24 h

Dose descriptor:

NOEC

Remarks:

(condition)

Effect conc.:

< 1 g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: Swimming behaviour, colour, any other visual observable morphological or behavioural criterion

Remarks on result:

other: The NOEC values were estimated by comparing effects with regard to mobility, according to the definition given in OECD 202 (ref. 1) and condition of the exposed animals with those of the control animals.

Details on results:

n the initial limit test all daphnias were immobile at a concentration of 10 g/l. In the full test there were no immobile daphnias at a concentration of 1.0 g/l, however, at this concentration the daphnias were slower, floating or had particles on their appandages.

It is likely that the results of the test are seriously affected by the high pH value.

- Behavioural abnormalities: At a test concentration of 1.0 g/l some daphnias were slower,
floating or had particles on their appandages. At test concentrations of 1.8 g/l and 3.2g/l some daphnias were slower or floating. At a test concentration of 5.6 g/l some daphnias were floating. - Observations on body length and weight: none
- Other biological observations: none
- Mortality of control: no
- Other adverse effects control: no
- Abnormal responses: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: The test substance appeared to be completely dissolved at all concentrations tested at the beginning of the test (visually assesed). After 24 h undissolved material was
observed on the bottom of the vessel at all concentrations tested.
- Effect concentrations exceeding solubility of substance in test medium: see above

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

A limit test according to OECD 202 did not show any adverse effects to Daphnia magna at a test concentration of 1 g/l L-arginine.
A subsequent test with L-arginine according to OECD 202 with higher test concentrations showed the following results:
EC50 = 1.8 g/l; EC100 = 5.6 g/l; NOEC (mobility) was estimated to be 1.0 g/l and NOEC (condition) was estimated to be < 1.0 g/l.

Executive summary: