Absolute of Nicotiana tabacum (Solanaceae) obtained from leaves by organic solvent treatment and subsequent ethanol extraction

Vapour pressure

Link to relevant study record(s)

Reference

Endpoint:

vapour pressure

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2019-2020

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 104 (Vapour Pressure Curve)

Version / remarks:

2006

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.4 (Vapour Pressure)

Version / remarks:

2009

Deviations:

no

Principles of method if other than guideline:

Not applicable.

GLP compliance:

yes (incl. QA statement)

Remarks:

Inspected on 15-16/11/2017, Signed on 15/05/2018

Type of method:

static method

Key result

Test no.:

#1

Temp.:

20 °C

Vapour pressure:

516 Pa

Key result

Test no.:

#2

Temp.:

25 °C

Vapour pressure:

670 Pa

Findings

Measurement Values

The measured values are presented in the following table.

Measurement Values, Date: 26. – 29.May 2020

1. Determination increasing temperature			2. Determination decreasing temperature
Temp. in °C	Time	Pressure in hPa	Temp. in °C	Time	Pressure in hPa
20.0	13:30	5.0	53.0	13:30	25.1
27.9	14:15	7.9	44.9	14:50	20.8
36.0	07:30	12.9	36.1	15:10	13.0
43.9	10:00	16.2	28.2	15:40	8.0
51.8	11:05	21.5	19.9	07:00	4.9
60.0	11:50	28.8	15.1	07:40	3.6

Results

The calculation results are presented in the following table:

Calculated values for 1/T and log P

n	Temperature T	Pressure p	Temperature T	1/T	log p
	°C	hPa	K	1/K	log Pa
1	20.0	5.0	293.15	0.00341122	2.6990
2	27.9	7.9	301.05	0.00332171	2.8976
3	36.0	12.9	309.15	0.00323468	3.1106
4	43.9	16.2	317.05	0.00315408	3.2095
5	51.8	21.5	324.95	0.00307740	3.3324
6	60.0	28.8	333.15	0.00300165	3.4594
7	53.0	25.1	326.15	0.00306607	3.3997
8	44.9	20.8	318.05	0.00314416	3.3181
9	36.1	13.0	309.25	0.00323363	3.1139
10	28.2	8.0	301.35	0.00331840	2.9031
11	19.9	4.9	293.05	0.00341239	2.6902
12	15.1	3.6	288.25	0.00346921	2.5563

 

The parameters for the linear regression of the graph log (p) vs. 1/T are presented in the following table:

Linear Regression Parameters

Parameter	Value
Slope	-1978.720994
Intercept	9.462700901
Correlation coefficient r	-0.990557148
Coefficient of determination r^2	0.981203463

 

Using the equation which is stated in chapter9, vapour pressures at ambient temperature were calculated as:

Calculated Values for vapour pressure

Parameter	Value	Unit
Vapour Pressure (20.0 °C)	516	Pa
Vapour Pressure (25.0 °C)	670	Pa

 

Conclusions:

The vapour pressure of the test item was calculated as 516 Pa at 20.0 °C and 670 Pa at 25.0 °C.

Executive summary:

An experimental study, conducted in accordance with conditions recommended in OECD guideline and under GLP, was performed. The vapour pressure was measured using the static method as described in EU method A.4.Twelve pairs temperature/vapour pressure were used to calculate a regression from which vapour pressure at ambient temperature could be estimated.

From the measured values, the vapour pressure of the test item was calculated as 516 Pa at 20.0 °C and 670 Pa at 25.0 °C. The values of the heating and cooling down phase showed good correspondence.

Therefore, the result of the study is considered valid.

Description of key information

The vapour pressure of the test item was calculated as 516 Pa at 20.0 °C and 670 Pa at 25.0 °C.

Key value for chemical safety assessment

Vapour pressure:

516 Pa

at the temperature of:

20 °C

Additional information

An experimental study, conducted in accordance with conditions recommended in OECD guideline and under GLP, is performed. It should be considered as a key study. The result should be retained as key data.