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EC number: 420-920-1 | CAS number: 128446-35-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
- Report date:
- 1997
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- .beta.-Cyclodextrin, 2-hydroxypropyl cycloheptaamylose
- IUPAC Name:
- .beta.-Cyclodextrin, 2-hydroxypropyl cycloheptaamylose
- Reference substance name:
- -
- EC Number:
- 420-920-1
- EC Name:
- -
- Cas Number:
- 128446-35-5
- Molecular formula:
- Hill formula: (C42H70-nO35)(C3H7O)n; n(mittel)=5,25
- IUPAC Name:
- 5,10,15,25-tetrakis(hydroxymethyl)-40,44,47,49-tetrakis(2-hydroxypropoxy)-20,30,35-tris[(2-hydroxypropoxy)methyl]-2,4,7,9,12,14,17,19,22,24,27,29,32,34-tetradecaoxaoctacyclo[31.2.2.2³,⁶.2⁸,¹¹.2¹³,¹⁶.2¹⁸,²¹.2²³,²⁶.2²⁸,³¹]nonatetracontane-36,37,38,39,41,42,43,45,46,48-decol
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- % Active: 93.5
Molecular weight: 1326-1400 g/mol
Solubility, in water: completely miscible
Color: white
Form: powder
TOC (mg TOC/mg active): 0.49
TCO2(mg CO2/mg active): 1.78
Storage Conditions: room temperature; dry, cool storage preferred
Expiration Date: 03/01/97
Date Received: 12/16/96
Constituent 1
Constituent 2
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- Twenty milliliters of the inoculum was added to all test flasks. The test flasks were placed on a rotary shaker, connected to the scrubbing train, and aerated overnight to purge the system of background CO2. After overnight aeration, three 4 oz.
CO2 absorber bottles (French squares) were filled with 100 mL of 0.024 N Ba(OH)2 and were connected in series to the exit air line of each test flask.
Activated sludge was collected from the DRWPCC on 13 January 1997. The sludge was screened through a 2 mm sieve prior to determining the total suspended solids (TSS). Based on this result approximately three litersof the sludge was adjusted to a target solids level
of 2,500 mg/L. The adjusted sludge was aerated in two semi-continuous activated sludge (SCAS)units until used on the following day in the preparation of the inoculum.
The inoculum was prepared for addition to the CO2 flasks as follows: Approximately 300 mL of activated sludge was collected from each SCAS unit, composited, and homogenized at medium speed in a blender for - 2 minutes. The homogenized sample was poured into a beaker and allowed to settle for ~ 30 minutes. The supematant· was decanted. and added to. the flasks at a concentration of 1% v/v. On the same day, a SPC was performed on the inoculum. The plates were incubated at test temperature. The result was 6.1 x 10*5 CFU/mL. - Duration of test (contact time):
- 34 d
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: 1,000 mg active/L stock solution was added to the duplicate flasks to achieve the specified concentration of 10 mg C/L
- Test temperature: 21.6-22.5°C
- pH: 7 +/- 1
- pH adjusted: yes
- Aeration of dilution water: yes
- Suspended solids concentration: 2,500 mg/L
TEST SYSTEM
- Culturing apparatus: flask
- Number of culture flasks/concentration:3
CONTROL:
- Abiotic sterile control: yes
- Toxicity control: yes
Results and discussion
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- < 10
- Sampling time:
- 34 d
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not inherently biodegradable
- Conclusions:
- The C02 produced from the reference substance exceeded the 60% of theoretical within 14 days necessary to consider the test valid.
- Executive summary:
A CO2 evolution test on test substance hydroxypropylated .beta.-cyclodextrin equivalent to OECD Guideline 301 A was conducted. This test was designed to determine the rate and extent of the ultimate biodegradation of the test substance under aerobic conditions.
The test apparatus consisted of five glass 4-liter Erlenmeyer flasks containing two liters of modified biochemical oxygen demand (BOD) water. Activated sludge was collected from the Downingtown Regional Water Pollution Control Center (DRWPCC) in Downingtown, Pennsylvania and used as the inoculum in this study. The sludge was not pre-exposed to the test substance in the laboratory prior to addition to the test flasks.
A dosing solution of hydroxypropylated .beta.-cyclodextrin was prepared in deionized (ASTM Type II) water at a concentration of 1,000 mg active/L. The solution was used to volumetrically dose duplicate flasks at a concentration of 10 mg C/L. Duplicate flasks receiving no test substance (blank control) and a single flask receiving sodium benzoate at a concentration of 10 mg C/L (reference· substance) were included in the test design. The flasks were placed on a rotary platform shaker and mixed at 110 ± 10 rpm for the duration of the study.
The CO2 produced in each flask reacted with 0.024 N Ba(OH)2 and precipitated as BaCO3.
The amount of CO2 produced was determined by titrating the remaining Ba(OH)2 with 0.05 N standardized hydrochloric acid (Hel). After 34 days the contents of the flasks were acidified with concentrated sulfuric acid (H2S04) and aerated overnight. One final titration was performed.
The C02 produced from the reference substance exceeded the 60% of theoretical within 14 days necessary to consider the test valid.
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