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EC number: 947-946-9
CAS number: -
as a Percentage of Theoretical CO2 Yield
Test Substance R1
Test Substance R2
Reference Substance R1
Reference Substance R2***
Numbers are rounded for presentation. Consequently, the displayed mean
may not be the calculated mean of the rounded individual values shown.
* Toxicity control values are corrected for mean test substance
Day 29 refers to the day that titrations of trap content from acidified
vessels were performed. Actual acidification was performed on Day 28.
Results for Reference Substance R2 are presented in the table, but were
excluded from the test conclusions due to an observed leak at the
beginning of the test; biodegradation in this replicate did not meet the
validity criterion for the procedure control.
ready biodegradability of the test substance was assessed by measurement
of carbon dioxide (CO2) evolution under standard conditions according to
OECD Test Guideline 301B. The
test substance was administered to the test system by direct addition. A
buffered synthetic, mineral salts medium prepared according to OECD
guideline 301B was added to give a test substance concentration
equivalent to 15 mg C/L. The concentration of suspended solids was
calculated to be 4.59 g/L. The medium was inoculated from a sample of
non-adapted activated sludge to give a suspended solids concentration of
30 mg/L in each control or test vessel. Test
vessels were incubated in darkness at 22 ± 2°C for 28 days and their
contents continuously sparged with a supply of CO2 free air (ca. 50 mL
per minute). The exhaust air from each vessel was passed through a
series of traps containing a barium hydroxide solution to trap evolved
Days 2, 5, 7, 9, 12, 14, 16, 19, 23, 26, 28, traps were removed from the
series and their contents titrated with hydrochloric acid to determine
the quantity of CO2 evolved from the respective test vessels. At the end
of incubation, 28 days, the test vessel contents were acidified to
release any residual CO2 that may have remained in solution. Titration
of the remaining traps on Day 29 was performed following overnight
of pH were made in the blank control and reference substance vessels at
the start of incubation and in all vessels at the end of the test prior
to the addition of the hydrochloric acid.
performance of the inoculum was checked by measuring the CO2 evolved
from procedure control vessels containing a reference substance, sodium
benzoate (15 mg C/L). An additional vessel containing a combination of
the test and reference substances (15 mg C/L) served as a toxicity
control to assess whether the test substance was inhibitory to
biodegradation at the test concentration. Two blank control vessels were
also prepared containing inoculated medium only to check the validity of
the test and to correct for baseline CO2 evolved. Duplicate vessels were
prepared for the test substance, reference substance and blank control
groups. A single vessel was prepared for the toxicity control.
biodegradation of the test substance reached 11% of the theoretical
carbon dioxide yield after 28 days. The
test substance therefore cannot be considered readily biodegradable.Percent
biodegradation values at each sampling interval, for the two replicates
containing the test substance did not vary by more than 15%, satisfying
the validity criterion of less than 20% difference.
mean total CO2 production in the blank control vessels was 40.9 mg/L at
the end of the test, satisfying the validity criterion of < 70 mg/L.
of the reference substance vessel (R1) exceeded 60% by Day 9 (64%) and
had reached a maximum of 83% by the end of the test. Biodegradation of
the reference substance in the presence of the test substance in the
toxicity control (61% at Day 12, 73% at the end of the incubation phase
on Day 28 and 75% by end of test on Day 29) was similar to that of the
reference substance alone, suggesting that the test substance did not
have an inhibitory effect on the inoculum under the test conditions, and
satisfying the validity criterion.
item attained 11% biodegradation after 28 days and therefore cannot be
considered to be readily biodegradable under the strict terms and
conditions of OECD Guideline No. 301B.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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