Registration Dossier
Registration Dossier
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Reaction products of diazotised 5-amino-2-anilinobenzenesulphonic acid coupled with resorcinol, subsequently coupled with diazotised 3-amino-4-hydroxy-5-nitrobenzenesulphonic acid, subsequently coupled with diazotised sodium 2-amino-4,6-dinitrophenoxide, subsequently coupled with diazotised 4-nitroaniline, sodium salts
EC number: 601-986-0 | CAS number: 12219-56-6
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
EC50 (48 h, Daphnia magna) > 37 mg/l (meas. (geom. mean))
NOEC (48 h, Daphnia magna) ≥ 37 mg/L (meas. (geom. mean))
Key value for chemical safety assessment
Additional information
A limit test was conducted to determine the acute toxicity of the test substance to Daphnia magna, according to the OECD Guideline 202 (2004) and the EU method C.2 of the Regulation EC 440/2008. The study was performed using the concentration of 100 mg/L test item (nominal). For the test concentration, 20 daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised daphnia were counted. The concentration showed no significant toxicity. Only 1 animal (5 %) was immobilised in the treatment which can be stated as not significant. None of the animals were immobilised in the blank control. Potassium dichromate was used as positive control in a current reference study to assure that the test conditions are reliable. At the beginning and at the end of the test, the content of the test item in the test solutions was determined using a photometer method. At the beginning of the test the measured concentration was 38 % of the nominal concentration and after 48 hours the measured test concentration was 37 % of the nominal concentration. Therefore, the determination of the biological results was based on the geometric mean of the measured concentrations.
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