Reaction products of diazotised 5-amino-2-anilinobenzenesulphonic acid coupled with resorcinol, subsequently coupled with diazotised 3-amino-4-hydroxy-5-nitrobenzenesulphonic acid, subsequently coupled with diazotised sodium 2-amino-4,6-dinitrophenoxide, subsequently coupled with diazotised 4-nitroaniline, sodium salts

Endpoint summary

Description of key information

Short-term toxicity to aquatic invertebrates:

EC50 (Daphnia magna, 48 h) > 37 mg/L (meas. (geom. mean))^*

Toxicity to aquatic plants other than algae:

ErC50 (Lemna minor, 7d, frond number) > 47.96 mg/L (meas. (geom. mean))^*

^* both values of geometric mean of measured concentrations correspond to 100 mg/L nominal concentration

Additional information

Firstly, it is worth saying that the substance is very water soluble (i.e. > 10 g/L); it has a log Kow lower than 4 (indicating a low potential for bioaccumulation) and is not rapidly degradable.

Short-term toxicity to aquatic invertebrates

A limit test was conducted to determine the acute toxicity of the test substance to Daphnia magna, according to the OECD Guideline 202 (2004) and the EU method C.2 of the Regulation EC 440/2008. The study was performed using the concentration of 100 mg/L test item (nominal). For the test concentration, 20 daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised daphnia were counted. The concentration showed no significant toxicity. Only 1 animal (5 %) was immobilised in the treatment which can be stated as not significant. None of the animals were immobilised in the blank control. Potassium dichromate was used as positive control in a current reference study to assure that the test conditions are reliable. At the beginning and at the end of the test, the content of the test item in the test solutions was determined using a photometer method. At the beginning of the test the measured concentration was 38 % of the nominal concentration and after 48 hours the measured test concentration was 37 % of the nominal concentration. Therefore, the determination of the biological results was based on the geometric mean of the measured concentrations.

Toxicity to aquatic plants other than algae

The acute toxicity of the test item to aquatic plants was investigated in a 7–day static test to Lemna minor, according to the OECD Guideline 221 (2006). The study was performed using 6 concentrations ranging from 0.32 to 100 mg/L (nominal concentration). The frond number of each replicate was determined at the beginning, at day 3 and 6 during the test and at the end of the experiment. Additionally, the dry mass of each replicate was determined at the beginning and at the end of the experiment. Growth rate µ and the yield were determined from the frond number and the dry mass at the respective observation times.

At the end of test, replicates with and without plants were analysed. The measured concentrations in the replicates with and without plants are in the same range. Therefore, the measured values in the replicates without plants were used for evaluation.

The measured concentrations lay between 22 % and 47 % of the nominal concentrations at the beginning of the test and between 20 % and 51 % of the nominal concentrations at the end of the test (without plants). Therefore, the determination of the NOEC / LOEC / ECx was based on the geometric mean of the measured concentrations. The 7d-EC50 values of positive control were determined in a separate reference test; the value of the growth rate lay within the desired range of 1.7 - 5.7 mg/L.

 

JUSTIFICATION FOR CLASSIFICATION OR NON-CLASSIFICATION

According to the CLP Regulation (EC n. 1272/2008), Part 4: Environmental Hazards, the substances can be classified for hazardous to the aquatic environment when the following criteria are met:

A) Acute (short-term) aquatic hazard Category Acute 1: 96 hr LC50 (for fish) and/or 48 hr EC50 (for crustacea) and/or 72 or 96 hr ErC50 (for algae or other aquatic plants) ≤ 1 mg/l.

B) Long-term aquatic hazard (iii) Substances for which adequate chronic toxicity data are not available and the substance is not rapidly degradable and/or the experimentally determined BCF ≥ 500 (or, if absent, the log K ow ≥ 4).

Category Chronic 1: 96 hr LC50 (for fish) and/or 48 hr EC50 (for crustacea) and/or 72 or 96 hr ErC50 (for algae or other aquatic plants) ≤ 1 mg/L

Category Chronic 2: 96 hr LC50 (for fish) and/or 48 hr EC50 (for crustacea) and/or 72 or 96 hr ErC50 (for algae or other aquatic plants)> 1 to ≤10 mg/L

Category Chronic 3: 96 hr LC50 (for fish) and/or 48 hr EC50 (for crustacea) and/or 72 or 96 hr ErC 50 (for algae or other aquatic plants) > 10 to ≤ 100 mg/L.

The substance is not rapidly degradable and the available acute toxicity test to Daphnia magna and Lemna minor fixed effect levels that do not meet these classification criteria. Therefore, the substance is not classified for aquatic toxicity according to the CLP Regulation (EC 1272/2008).