Montan wax

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study according to OECD test guideline 201 under GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Montan wax

EC Number:

232-313-5

EC Name:

Montan wax

Cas Number:

8002-53-7

Molecular formula:

As montan wax is a UVCB, a molecular formular cannot be specified.

IUPAC Name:

Montan wax

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
The stock solution of 100 mg/L was prepared with dilution water and shaken with 20 rpm for 48 h at room temperature. After a
separation phase of 1 h the water accomodated fraction (WAF) was taken from the homogenous liquid phase.

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Strain:CHODAT SAG 86.81
- Source (laboratory, culture collection): Sammlung von Algenkulturen (SAG), Pflanzenphysiologisches Institut der Universit8t GOttingen,
Nikolausberger Weg 18, D-37073 Goettingen


ACCLIMATION
- Acclimation period: 3 days

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Test conditions

Test temperature:

Nominal range: 21 — 24 °C, controlled at ± 2 °C

pH:

7.62 - 8.53

Nominal and measured concentrations:

A water accommodated fraction (WAF) of 100 mg/L was prepared. Per definition of the WAF all terms related to concentration levels will be given as loading levels because partly dissolved compounds and mixtures cannot be related to concentrations. One loading level of 100 mg/L was tested in a limit test.

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Duration:

72 h

Dose descriptor:

EC0

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

Microscopic evaluation of the cells at start and end of the incubation period revealed no morphological abnormalities

Results with reference substance (positive control):

Reference item poassium dichromate was tested on September 22, 2008.
Growth inhibition EC50 was 0.69 mg/L lying in the valid range of 0.828 +/-0.399.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In a study according to OECD test guideline 201 under GLP, the EC50-value of the test substance for algal growth inhibation was determined to be >100mg/l.

Executive summary:

In a study according to OECD test guideline 201 under GLP the effects of the test substance on the growth of the freshwater green alga Desmodesmus subspicatus were tested. Slight inhibiting effects on the yield and no effects on the specific growth rate were found in the water accommodated fraction of 100 mg/L. The EC50-values for specific growth rate inhibition and inhibition of yield (EyL50) after 72 h were > 100 mg/L.