Montan wax

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study according to OECD Test Guideline 202 under GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

temperature marginally above guideline limits

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Montan wax

EC Number:

232-313-5

EC Name:

Montan wax

Cas Number:

8002-53-7

Molecular formula:

As montan wax is a UVCB, a molecular formular cannot be specified.

IUPAC Name:

Montan wax

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Details on test solutions:

As solubility lies below 100 mg/L, a saturated solution was prepared for the test. This was done by weighing the nominal load, adding the corresponding amount of dilution water and shaking vigorously for 24 hours. The resulting solution was filtrated through 0.45 µm filters.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Strain: Straus
- Source: Umweltbundesamt Berlin, Germany
- Age at study initiation (mean and range, SD): 0-24 hours
- Method of breeding: Daphnia magna is bred in the LAUS GmbH throughout the year.
- Feeding during test: no

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

2.5 mmol/l

Test temperature:

21.9 to 23.3 °C

pH:

7.6 to 8.8

Dissolved oxygen:

> 8.4 mg/L

Nominal and measured concentrations:

Because of the low water solubility of the test item, the measured values for treatment and control were in the same range. Therefore, the determination of the biological results was based on the nominal concentration.

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: no
- Mortality of control: no
- Other adverse effects control: no
- Abnormal responses: no

Results with reference substance (positive control):

- Results with reference substance valid? Yes
- EC50/LC50: 0.83 mg/L (24h)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In a study according to OECD test guideline 202 under GLP, the acute toxicity EC50 to Daphnia magna of the test substance was determined to be > 100 mg/L.

Executive summary:

In a study according OECD test guideline 202 under GLP, the limit test test substance containing of 100 mg/L was used (nominal concentration). Twenty daphnia were exposed to the test substance for 48 hours in a static test system. After 24 and 48 hours, the immobilised daphnia were counted. The treatment showed no toxicity. No animals were immobilised in the control.

The 24h-EC50i of potassium dichromate was tested in a current positive control test. The value was determined as 0.83 mg/L, lying within the demanded range of 0.6 — 1.7 mg/L.

At the beginning and at the end of the test, the content of test item was determined by calculation from content of organic carbon of the test item (80.4 %) and DOC-determination in the test solutions. Because of the low water solubility of the test item, the measured DOC values of treatment and control were in the same range.

Therefore the determination of the biological results was based on the nominal concentration.

The EC50 of the test substance was determined to be > 100 mg/L (nominal).