Registration Dossier
Registration Dossier
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EC number: 619-290-0 | CAS number: 97780-06-8
Toxicity to terrestrial arthropods
Administrative data
- Endpoint:
- toxicity to terrestrial arthropods: long-term
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 232 (Collembolan Reproduction Test in Soil)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 11267 (Inhibition of Reproduction of Collembola by Soil Pollutants)
- Deviations:
- no
- GLP compliance:
- yes
- Application method:
- soil
Test material
- Reference substance name:
- Reference substance 002
- Cas Number:
- 97780-06-8
- Test material form:
- solid
- Details on test material:
- - Purity: 98.7%
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test substrate
- Vehicle:
- yes
- Remarks:
- acetone
Test organisms
- Test organisms (species):
- Folsomia candida
- Animal group:
- Collembola (soil-dwelling springtail)
Study design
- Study type:
- laboratory study
- Limit test:
- no
- Total exposure duration:
- 28 d
Test conditions
- Test temperature:
- 19 to 21°C
- pH (if soil or dung study):
- At test start: 6.3 to 6.4
At test end: 6.3 to 6.5 - Photoperiod and lighting:
- 16 h light : 8 h dark
- Nominal and measured concentrations:
- Nominal: 6.25, 12.5, 25.0, 50.0 and 100 mg/kg soil dry weight, adjusted for purity
- Reference substance (positive control):
- yes
- Remarks:
- boric acid
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 28 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: Highest dose tested
- Key result
- Duration:
- 28 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: Highest dose tested
- Key result
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: mortality and reproduction
- Remarks on result:
- other: Highest dose tested
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- 28 d EC50 and LOEC (Folsomia candida): >100 mg/kg soil dw (Highest concentration tested)
28 d NOEC (Folsomia candida): 100 mg/kg soil dw (Highest concentration tested) - Executive summary:
The effects of the test substance on survival and reproduction of Collembola, Folsomia candida, was measured in a 28-day soil exposure GLP-compliant laboratory study based on OECD 232 and ISO 11267. Eight replicates for the control and four replicates per test item group, containing ten Collembola each (total 80 per control and 40 individuals per test substance group) were each exposed for 28 days to the nominal concentrations of 6.33, 12.66, 25.33, 50.66 and 101.3 mg test substance/kg soil dry weight (corresponding to 6.25, 12.5, 25.0, 50.0 and 100 mg test substance/kg soil dry weight, adjusted for purity) and to an untreated control (using the same amount of acetone and fine quartz sand per g substrate as in the test item groups). The concentrations of test substance adjusted for purity will be used to determine the endpoints of the test. After 28 days exposure the Collembola were assessed for the number of surviving adults (mortality) and the number of juveniles (reproduction).
The reference item boric acid is tested once a year. In the most recent test performed, the EC50 for reproduction was determined to be 107.2 mg boric acid/kg soil dry weight.
No significant effects were observed up to and including the concentration of 100 mg/kg soil dry weight.
The test substance had no significant lethal or reproductive effects on the Collembola Folsomia candida when exposed to concentrations up to and including 100 mg/kg artificial soil dry weight.
The 28 day EC50 and the Lowest-Observed-Effect Concentration (LOEC) for the test substance were determined to be greater than 100 mg/kg artificial soil dry weight. The overall 28 day No-Observed-Effect Concentration (NOEC) based on mortality and reproduction was determined to be 100 mg/kg artificial soil dry weight.
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