Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 619-290-0 | CAS number: 97780-06-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1075 (Freshwater and Saltwater Fish Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Reference substance 002
- Cas Number:
- 97780-06-8
- Test material form:
- solid
- Details on test material:
- Purity: 99.2%
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Samples were collected from each test chamber at test initiation and termination to measure concentrations of the test substance. The samples were collected at mid-depth and placed in glass vials. Two sets of samples were collected at each interval. One set was processed immediately for analysis. The second set was stored refrigerated for possible future analysis.
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test solution for each test chamber was prepared, individually, at a nominal concentration of 120 mg active substance/L. The solution was prepared by mixing calculated amounts of the test substance into 25 L of dilution water (well water). Test solutions were adjusted to 100% active substance based upon the reported test substance purity of 99.2%. Prior to test solutions preparation, 25-L of dilution water was measured into each test chamber, using a calibrated plastic vessel. The test substance was weighed into a tared beaker and rinsed directly into the 25-L dilution water in each treatment test chamber. The test substance and dilution water were mixed using a top-down electric mixer for approximately 21 hours. At the beginning of the mixing period, test solution at 120 mg a.s./L was gray and slightly opaque with white precipitate observed on the surface of the solution. A glass pasteur pipette was used to rinse the test substance adhering to the sides of the test chambers into the solutions. At the end of the mixing, all solutions appeared clear and colorless.
- Controls: The negative control was dilution water only
- Chemical name of vehicle: Dilution water
Test organisms
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow trout
- Strain: Oncorhynchus mykiss
- Source: Thomas Fish Company, Anderson, California
- Age at dosing: Juvenile
- Length at end of the test (negative control fish): 4.3 to 5.1 cm
- Weight at end of the test (negative control fish): 0.76 to 1.6 grams
ACCLIMATION
- Acclimation period: 14 days
- Type and amount of food during acclimation: Fish were fed commercially-prepared diet, and were not fed for at least two days prior to the test or during the test.
- Health during acclimation: During the 2-week period prior to the test the fish showed no signs of disease or stress and no mortalities occurred during the 7 days prior to the test.
FEEDING DURING TEST
- Frequency: Unfed
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 136 mg/L as CaCO3
- Test temperature:
- 11.7 to 12.8°C
- pH:
- 8.1 to 8.5
- Dissolved oxygen:
- ≥7.8 mg/L (72% of saturation)
- Conductivity:
- 396 μS/cm
- Nominal and measured concentrations:
- Nominal: 120 mg a.s./L
Mean measured: 126 mg a.s./L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 38-L stainless steel aquaria containing 25 L of test solution
- No. of organisms per vessel: 10 fish
- No. of vessels per concentration (replicates): 3
- Aeration: gentle aeration was initiated in each test chamber to ensure that dissolved oxygen concentrations remained above 60% saturation through test termination.
- Loading: 0.52 g fish/L (wet weight of fish per liter of test water)
TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: The water used for culturing and testing was obtained from a well approximately 40 meters deep located on the test facility site. The well water was passed through a sand filter to remove particles greater than approximately 25 μm, and pumped into a 37800 L storage tank where the water was aerated with spray nozzles. Prior to use, the water was filtered to 0.45 μm and passed through an ultraviolet sterilizer to remove fine particles and microorganisms. The well water is characterized as moderately-hard water.
- Alkalinity: 180 mg CaCO3/L
OTHER TEST CONDITIONS
- Photoperiod: 16 hr photoperiod (218 lux at initiation) and 8 hr darkness which included 30-minute transition period of low light intensity was provided when lights went on and off to avoid sudden changes in lighting.
EFFECT PARAMETERS MEASURED: Observations were made periodically to determine the number of dead organisms in each treatment group. Lethality was defined as a lack of visible movement by the test organism. The numbers of individuals exhibiting signs of toxicity or abnormal behaviour also were evaluated. Observations were made approximately 2.5, 24, 48, 72 and 96 hours after test initiation. - Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 126 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Details on results:
- All trout in the negative control group and the 126 mg a.s./L test groups appeared normal throughout the test, with no mortality or sublethal effects observed.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- 96-hour LC50 (Oncorhynchus mykiss): >126 mg a.s./L (mortality)
- Executive summary:
The acute toxicity of the test substance to unfed juvenile rainbow trout (Oncorhynchus mykiss), a freshwater fish, was determined in a static, 96-hour limit test. The test was conducted in accordance with the OECD Guideline for Testing of Chemicals, 203, Fish Acute Toxicity Test and U.S. Environmental Protection Agency Series 850 –Ecological Effects Test Guidelines, OPPTS Number 850.1075, Fish Acute Toxicity Test, Freshwater and Marine. Treatments consisted of a dilution water control and a nominal test substance concentration of 120 mg active substance/L. The corresponding mean, measured concentration was 126 mg a.s./L. All trout in the negative control group and the 126 mg a.s./L test groups appeared normal throughout the test, with no mortality or sublethal effects observed. The 96-hour LC50 for rainbow trout, Oncorhynchus mykiss, based on the mean, measured concentration and mortality was greater than 126 mg a.s./L, the limit test concentration.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.