Phosphorodithioic acid, O,O-di-C1-14-alkyl esters, zinc salts

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

secondary literature

Justification for type of information:

Data is from IUCLID data set

Data source

Reference

Reference Type:

secondary source

Title:

Acute dermal toxicity study in rabbits

Author:

Toxic Substances Control Act Test Submissions (TSCATS)

Year:

2006

Bibliographic source:

United States Environmental Protection Agency (EPA) 2006

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: as below

Principles of method if other than guideline:

Acute dermal toxicity study of Phosphorodithioic acid, O,O-di-C1-14-alkyl esters, zinc salts (89-92 %) in rabbits

GLP compliance:

not specified

Test type:

other: not specified

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Phosphorodithioic acid, O,O-di-C1-14-alkyl esters, zinc salts

EC Number:

272-028-3

EC Name:

Phosphorodithioic acid, O,O-di-C1-14-alkyl esters, zinc salts

Cas Number:

68649-42-3

Molecular formula:

C28H60O4P2S4Zn

IUPAC Name:

Dialkyl(C1-C14)dithiophosphoric acid, zinc salt

Test material form:

liquid

Details on test material:

- Name of test material (IUPAC name): Dialkyl(C1-C14)dithiophosphoric acid, zinc salt
- Common name: Phosphorodithioic acid, O,O–di–C1–14–alkyl esters, zinc salts
- Molecular formula: C28H60O4P2S4Zn
- Molecular weight: 716.38 g/mol
- Smiles notation: [Zn+2].CCCCCCCOP(=S)([S-])OCCCCCCC.CCCCCCCOP(=S)([S-])OCCCCCCC
- InChl: 1S/2C14H31O2PS2.Zn/c2*1-3-5-7-9-11-13-15-17(18,19)16-14-12-10-8-6-4-2;/h2*3-14H2,1-2H3,(H,18,19);/q;;+2/p-2
- Substance type: Organic
- Physical state: liquid

Specific details on test material used for the study:

- Name of test material (IUPAC name): Dialkyl(C1-C14)dithiophosphoric acid, zinc salt
- Common name: Phosphorodithioic acid, O,O–di–C1–14–alkyl esters, zinc salts
- Molecular formula: C28H60O4P2S4Zn
- Molecular weight: 716.38 g/mol
- Smiles notation: [Zn+2].CCCCCCCOP(=S)([S-])OCCCCCCC.CCCCCCCOP(=S)([S-])OCCCCCCC
- InChl: 1S/2C14H31O2PS2.Zn/c2*1-3-5-7-9-11-13-15-17(18,19)16-14-12-10-8-6-4-2;/h2*3-14H2,1-2H3,(H,18,19);/q;;+2/p-2
- Substance type: Organic
- Physical state: liquid

Test animals

Species:

rabbit

Strain:

not specified

Sex:

male/female

Details on test animals or test system and environmental conditions:

not specified

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

not specified

Duration of exposure:

single

Doses:

200 and 3160 mg/kg bw

No. of animals per sex per dose:

Total: 8
200 mg/kg bw: 2 male, 2 female
3160 mg/kg bw: 2 male, 2 female

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: on day fist, 7 and on 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight and gross pathology were examined.

Statistics:

not specified

Results and discussion

Preliminary study:

not specified

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 3 160 mg/kg bw

Based on:

test mat.

Remarks on result:

other: No effect on survival

Mortality:

No mortality were observed in treated rabbits at 3160 mg/kg bw

Clinical signs:

other: Hypo-activity were observed in treated rabbits at 3160 mg/kg bw. The observed reaction was subside within 4 hours.

Gross pathology:

No gross pathological changes were observed in treated rabbits.

Other findings:

Moderate irritation were observed at 200 mg/kg bw and severe irritation at 3160 mg/kg bw in treated rabbits.

Red well-defined erythema and sever edema at 24 hours, moderate to severe desquamation and fissuring at day 7 and mild to moderate desquamation at day 14 were observed in 200 mg/kg bw treated rabbits.

Beet red erythema, sever edema and second degree burns at 24 hour, escharosis fissuring and hemorrhaging at day 7 and escharosis, necrosis and sever desquamation at day 14 were observed in 3160 mg/kg bw treated rabbits.

Any other information on results incl. tables

Acute Dermal Toxicity Study – Albino Rabbits

 

Dose Level (mg/kg)	Animal number	Individual Body Weight (Kg)			Number Dead/Number Tested	Percent Dead
		Test Day Number:
		0	7	14
200	1-M 2-M 3-F 4-F	3.00 3.30 3.20 3.42	3.00 3.20 3.18 3.50	3.12 3.30 3.38 3.54	0/4	0
3160	5-M 6-M 7-F 8-F	2.92 3.08 3.06 3.68	2.82 2.64 2.18 2.28	3.10 2.78 2.24 2.04	0/4	0

 

NOTE: The Test Skin Sites Of all Rabbits Were Abraded.

Applicant's summary and conclusion

Interpretation of results:

Category 5 based on GHS criteria

Conclusions:

LD50 was considered to be 3160 mg/kg bw when male and female rabbits were exposed with Phosphorodithioic acid, O,O-di-C1-14-alkyl esters, zinc salts on abraded skin.

Executive summary:

In a acute dermal toxicity study,male and female rabbits were treated with Phosphorodithioic acid, O,O-di-C1-14-alkyl esters, zinc salts in the concentration of200 and 3160 mg/kg bw applied onabraded skinand observed for 14 days.No mortality were observed in treated rabbits at 3160 mg/kg bw. Hypo-activity were observed in treated rabbits at 3160 mg/kg bw. The observed reaction was subside within 4 hours. In one male rabbit weight loss was observed during first week of treatment at 3160 mg/kg bw, which was resumed normal during second week. One female rabbit lost weight throughout 14 day of treatment. Moderate irritation were observed at 200 mg/kg bw and severe irritation at 3160 mg/kg bw in treated rabbits. Red well-defined erythema and sever edema at 24 hours, moderate to severe desquamation and fissuring at day 7 and mild to moderate desquamation at day 14 were observed in 200 mg/kg bw treated rabbits. Beet red erythema, sever edema and second degree burns at 24 hour, escharosis fissuring and hemorrhaging at day 7 and escharosis, necrosis and sever desquamation at day 14 were observed in 3160 mg/kg bw treated rabbits. No gross pathological changes were observed in treated rabbits. Therefore,LD50 was considered to be3160 mg/kg bw when male and female rabbits were exposed with Phosphorodithioic acid, O,O-di-C1-14-alkyl esters, zinc salts on abraded skin.