Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Acute Toxicity: oral

Currently viewing:

Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
secondary literature
Justification for type of information:
Data is from TSCATS

Data source

Reference
Reference Type:
secondary source
Title:
Acute oral study in rats
Author:
Toxic Substances Control Act Test Submissions (TSCATS)
Year:
2006
Bibliographic source:
United States Environmental Protection Agency (EPA),2006

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: as below
Principles of method if other than guideline:
Acute oral toxicity study of Phosphorodithioic acid, O,O-di-C1-14-alkyl esters, zinc salts (89-92 %) in male rats
GLP compliance:
not specified
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Phosphorodithioic acid, O,O-di-C1-14-alkyl esters, zinc salts
EC Number:
272-028-3
EC Name:
Phosphorodithioic acid, O,O-di-C1-14-alkyl esters, zinc salts
Cas Number:
68649-42-3
Molecular formula:
C28H60O4P2S4Zn
IUPAC Name:
Dialkyl(C1-C14)dithiophosphoric acid, zinc salt
Test material form:
liquid
Details on test material:
- Name of test material (IUPAC name): Dialkyl(C1-C14)dithiophosphoric acid, zinc salt
- Common name: Phosphorodithioic acid, O,O–di–C1–14–alkyl esters, zinc salts
- Molecular formula: C28H60O4P2S4Zn
- Molecular weight: 716.38 g/mol
- Smiles notation: [Zn+2].CCCCCCCOP(=S)([S-])OCCCCCCC.CCCCCCCOP(=S)([S-])OCCCCCCC
- InChl: 1S/2C14H31O2PS2.Zn/c2*1-3-5-7-9-11-13-15-17(18,19)16-14-12-10-8-6-4-2;/h2*3-14H2,1-2H3,(H,18,19);/q;;+2/p-2
- Substance type: Organic
- Physical state: liquid
Specific details on test material used for the study:
- Name of test material (IUPAC name): Dialkyl(C1-C14)dithiophosphoric acid, zinc salt
- Common name: Phosphorodithioic acid, O,O–di–C1–14–alkyl esters, zinc salts
- Molecular formula: C28H60O4P2S4Zn
- Molecular weight: 716.38 g/mol
- Smiles notation: [Zn+2].CCCCCCCOP(=S)([S-])OCCCCCCC.CCCCCCCOP(=S)([S-])OCCCCCCC
- InChl: 1S/2C14H31O2PS2.Zn/c2*1-3-5-7-9-11-13-15-17(18,19)16-14-12-10-8-6-4-2;/h2*3-14H2,1-2H3,(H,18,19);/q;;+2/p-2
- Substance type: Organic
- Physical state: liquid

Test animals

Species:
rat
Strain:
other: Charles River
Sex:
male
Details on test animals or test system and environmental conditions:
not specified

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
unchanged (no vehicle)
Details on oral exposure:
not specified
Doses:
1350, 2025, 3038, 4556, 5000 and 6834 mg/kg bw
No. of animals per sex per dose:
Total: 35
1350 mg/kg: 5 male
2025 mg/kg: 5 male
3038 mg/kg: 5 male
4556 mg/kg: 5 male
5000 mg/kg: 10 male
6834 mg/kg: 5 male
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: on day fist and on 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight and gross pathology were examined.
Statistics:
not specified

Results and discussion

Preliminary study:
not specified
Effect levels
Sex:
male
Dose descriptor:
LD50
Effect level:
3 195 mg/kg bw
Based on:
test mat.
Remarks on result:
other: No effect on survival, body weight, gross pathology
Mortality:
When treated with 6834 mg/kg bw, all animals died within 6-22 hours.

When treated with 5000 mg/kg bw, 7 animals died within 3-4 days.

When treated with 4556 mg/kg bw, 4 animals died within 2-3 days.

When treated with 3038 mg/kg bw, 2 animals died in 3 days.

When treated with 2025 mg/kg bw, 1 animals died in 3 days.

When treated with 1350 mg/kg bw, no mortality were observed in treated rats.
Clinical signs:
other: Hypoactivity, ptosis, ruffed fur, muscular weakness and Diarrhea were observed in treated rats.
Gross pathology:
Slightly reddened lungs were observed in dead animals and no gross pathological changes were observed in terminally sacrificed rats.
Other findings:
not specified

Any other information on results incl. tables

1.      Mortality and Body Weight Data:

Dose Level

(mg/kg)

Animal number

Individual Body Weight (grams)

Number Dead/Number Tested

Percent Dead

Test day number:

0

14

1350

1

2

3

4

5

196

214

22

204

216

306

342

364

320

346

0/5

0

2025

6

7

8

9

10

260

298

254

274

312

358

(3 days)

340

390

420

1/5

20

3038

11

12

13

14

15

320

288

246

354

260

396

366

308

(3 days)

(3 days)

2/5

40

4556

16

17

18

19

20

246

246

288

256

320

368

(6-22 hours)

(3 days)

(2 days)

(3 days)

4/5

80

5000

21

22

23

24

25

26

27

28

29

30

180

188

190

200

184

164

184

178

186

192

(3 days)

266

(3 days)

308

(4 days)

(4 days)

(3 days)

(4 days)

290

(4 days)

7/10

70

6834

31

32

33

34

35

318

302

244

300

264

(6-22 hours)

(6-22 hours)

(6-22 hours)

(2 days)

(6-22 hours)

5/5

100

              

Note: Figures in parentheses indicate time of death

 

 2.      Reactions and Pathology

 The pharmacotoxic symptoms exhibited by the rats post-oral administration of MRD-ECH-75-19 are presented below:

 

 

Reaction

Dose Levels (mg/kg)

1350

2025

3038

4556

5000

6834

O

R

O

R

O

R

O

R

O

R

O

R

HYPOACTIVITY

-

-

4 H

2 D

4 H

3 D

4 H

3 D

4 H

4 D

2 H

N

PTOSIS

-

-

-

-

4 H

3 D

4 H

3 D

4 H

4 D

2 H

N

RUFFED FUR

-

-

6-22 H

2 D

4 H

3 D

4 H

3 D

4 H

4 D

2 H

N

MUSCULAR WEAKNESS

-

-

6-22 H

2 D

4 H

3 D

4 H

3 D

4 H

4 D

2 H

N

DIARRHEA

-

-

6-22 H

2 D

1 H

2 D

1 H

2 D

1 H

4 D

1 H

N

 O = Onset           H = Hours   M =Minutes

R = Recovery      D = Days     N =No recovery    - = No reaction

Applicant's summary and conclusion

Interpretation of results:
Category 5 based on GHS criteria
Conclusions:
LD50 was considered to be 3195 mg/kg bw when CD male rats were treated with Phosphorodithioic acid, O,O-di-C1-14-alkyl esters, zinc salts orally by gavage.
Executive summary:

In a acute oral toxicity study,Charles River male rats were treated with Phosphorodithioic acid, O,O-di-C1-14-alkyl esters, zinc salts in the concentration of1350, 2025, 3038, 4556, 5000 and 6834 mg/kg bw orally by gavage and observed for 14 days. All animals died within 6-22 hours at 6834 mg/kg bw, 7 animals died within 3-4 days at 5000 mg/kg bw, 4 animals died within 2-3 days at 4556 mg/kg bw, 2 animals died in 3 days at 3038 mg/kg bw, 1 animals died in 3 days at 2025 mg/kg bw and no mortality were observed in treated rats at 1350 mg/kg bw. Hypoactivity, ptosis, ruffed fur, muscular weakness and Diarrhea were observed in treated rats. Slightly reddened lungs were observed in dead animals and no gross pathological changes were observed in terminally sacrificed rats. Therefore,LD50 was considered to be3195 mg/kg bw when CD male rats were treated with Phosphorodithioic acid, O,O-di-C1-14-alkyl esters, zinc salts orally by gavage.