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EC number: 201-029-3 | CAS number: 77-47-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1977
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- Limited number of animals per concentration; low hardness
- GLP compliance:
- no
- Specific details on test material used for the study:
- TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: test compound dissolved in acetone - Analytical monitoring:
- not specified
- Details on sampling:
- - Concentrations: 0.24 mg/L; 0.16 mg/L; 0.10 mg/L; 0.065 mg/L; 0.042 mg/L; 0.028 mg/L; 0.018 mg/L; 0.012 mg/L.
- Vehicle:
- yes
- Remarks:
- acetone
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: For each test concentration, the appropriate amount of test compound, dissolved in acetone, was pipetted into 500 ml of diluent water and mixed with a magnetic stirrer. The remaining 50 ml were used for 0-hour pH and DO determinations.
- Controls: Negative controls (same dilution water and conditions but with no test substance or acetone) and positive controls (containing greatest amount of acetone introduced into any one test vessel).
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Acetone - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Source: Laboratory stocks cultured at Bionomics
- Age of parental stock: <= 24 hours - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 35 mg/L CaCO3
- Test temperature:
- 21 ± 1 °C
- pH:
- 7.1
- Dissolved oxygen:
- > 60% saturation
- Nominal and measured concentrations:
- Nominal concentrations (mg/L): 0.24; 0.16, 0.10; 0.065; 0.042; 0.028; 0.018; 0.012.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 ml beaker
- Material, size, headspace, fill volume: 250 ml beaker containing 150 ml test solution
- Aeration: Test solutions were not aerated during the tests
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 3
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Well water was used to culture the water fleas. Prior to the test, diluent water was filtered through polyester fiber and sterilized with an ultraviolet light to remove any microorganisms.
- Intervals of water quality measurement: Dissolved oxygen concentration, pH and temperature of test solutions were checked at 0 and 48 hours in the highest, middle and lowest test concentrations, at a minimum.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : percentage mortality at 24 and 48 hours
VEHICLE CONTROL PERFORMED: yes
RANGE-FINDING STUDY
- Test concentrations: The preliminary test defined a narrower range of concentrations to be used in the definitive test which would then assess the dose-response relationship and permit calculation of an LC50 and its 95% confidence interval from the mortality data produced. - Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.13 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.039 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.018 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- - Other biological observations: All dead water flea observed during this exposure were carrying a partially attached carapace, suggesting the organisms died while attempting to moult.
- Mortality of control: No mortality
- Other adverse effects control: No effects - Reported statistics and error estimates:
- LC50 (24hr) = 0.068 - 0.26 mg/L (95% confidence interval)
LC50 (48hr) = 0.030 - 0.052 mg/L (95% confidence interval) - Validity criteria fulfilled:
- yes
- Conclusions:
- The 48 hr LC50 of hexachlorocyclopentadiene for Daphnia magna was 0.039 mg/L.
- Executive summary:
The short-term toxicity of hexachlorocyclopentadiene to the freshwater invertebrate species Daphnia magna was determined using a procedure equivalent to the OECD Guideline for Testing of Chemicals 202, with acceptable restrictions. The study determined the average percent mortality over a range of concentrations between 0.012 - 0.24 mg/L. It was determined that the 48 hr LC50 of the registered substance was 0.039 mg/L.
Reference
Table showing concentrations tested and corresponding average observed percentage mortalities for the water flea (Daphnia magna) exposed to hexachlorocyclopentadiene:
Nominal concentration (mg/L) | Average percentage mortality | |
24 hr | 48 hr | |
0.24 | 87 | 100 |
0.16 | 33 | 100 |
0.10 | 33 | 100 |
0.065 | 40 | 93 |
0.042 | 7 | 57 |
0.028 | 0 | 20 |
0.018 | 0 | 0 |
0.012 | 0 | 0 |
control (acetone) | 0 | 0 |
control | 0 | 0 |
Description of key information
Two non-GLP short-term toxicity studies were performed according to a method similar to the OECD Testing Guideline 202 on Daphnia magna. The lowest 48-hour EC50 was 0.039 mg/l. It was selected as the key value as it represents a worst-case scenario.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.039 mg/L
Additional information
The short-term toxicity of the registered substance to marine invertebrates was not investigated as part of this REACH Registration. It was not considered as relevant taking into account the uses of the substance.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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