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EC number: 201-029-3 | CAS number: 77-47-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1977
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- yes
- Remarks:
- Data on light was not recorded
- GLP compliance:
- no
- Specific details on test material used for the study:
- TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: test compound dissolved in acetone - Analytical monitoring:
- not specified
- Details on sampling:
- - Concentrations: 0.32 mg/L; 0.24 mg/L; 0.18 mg/L; 0.14 mg/L; 0.10 mg/L; 0.087 mg/L; 0.065 mg/L.
- Vehicle:
- yes
- Remarks:
- acetone
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test compound for each concentration, dissolved in acetone, was added directly into each jar and mixed by stirring.
- Controls: Negative controls (same dilution water and conditions but with no test substance or acetone) and positive controls (containing greatest amount of acetone introduced into any one test vessel).
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Acetone - Test organisms (species):
- Lepomis macrochirus
- Details on test organisms:
- TEST ORGANISM
- Common name: Bluegill
- Source: commercial fish hatchery in Nebraska
- Length at study initiation (length definition, mean, range and SD): 26 ± 4.9 mm
- Weight at study initiation (mean and range, SD): 0.45 ± 0.17 g
- Maintenance of the brood fish: fish were held in 1700L concrete raceways, coated with an epoxy resin paint to prevent leaching of extraneous materials into the water. Well water flowed through these raceways at a minimum of 4 L / minute, providing an adequate water turn-over rate for maintaining the species.
ACCLIMATION
- Acclimation period: 48 hours
- Acclimation conditions (same as test or not): test conditions of temperature and water quality
- Feeding frequency during acclimation: fish were not fed during acclimation period
- Health during acclimation (any mortality observed): no mortality observed and fish appeared to be in excellent condition at the initiation of the tests
QUARANTINE
- Duration: 30 days
- Health/mortality: less than 2% - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 35 mg/L CaCO3
- Test temperature:
- 21 ± 1 °C
- pH:
- 7.1
- Dissolved oxygen:
- > 60% saturation
- Details on test conditions:
- TEST SYSTEM
- Test vessel: jars
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume: 19.6 L glass jars containing 15 L test solution
- Aeration: Test solutions were not aerated during the tests.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Intervals of water quality measurement: Dissolved oxygen concentration, pH and temperature of test solutions were checked at 0, 24, 48 and 96 hours of exposure in the highest, middle and lowest test concentrations, at a minimum.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : percentage mortality at 24, 48, and 96 hours
TEST CONCENTRATIONS
- Range finding study: The preliminary test defined a narrower range of concentrations to be used in the definitive test which would then assess the dose response relationship and permit calculation of LC50 and its 95% confidence interval from the mortality data produced.
- Test concentrations: 0.32 mg/L; 0.24 mg/L; 0.18 mg/L; 0.14 mg/L; 0.10 mg/L; 0.087 mg/L; 0.065 mg/L. - Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.17 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.15 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.13 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.065 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Other biological observations: The mortality syndrome among fish from those concentrations in which mortality was observed was similar. Fish generally became dark and lethargic, lost equilibrium, and expired.
- Mortality of control: no mortality
- Other adverse effects control: no effects - Reported statistics and error estimates:
- LC50 (24hr) = 0.14 - 0.21 mg/L (95% confidence interval)
LC50 (48hr) = 0.12 - 0.18 mg/L (95% confidence interval)
LC50 (96hr) = 0.11 - 0.17 mg/L (95% confidence interval) - Sublethal observations / clinical signs:
Table showing concentrations tested and corresponding observed percentage mortalities for bluegill (Lepomis macrochirus)
Nominal concentration (mg/L) % mortality observed 24 h 48 h 96 h 0.32 100 100 100 0.24 80 (a,b) 100 100 0.18 30 (a,b) 30 (a,b) 40 (b) 0.14 10 (a,b) 30 (a,b) 40 (b) 0.10
10 10 20 (b) 0.087
0
0
10
0.065
0
0
0
control (acetone)
0
0
0
control
0
0
0
(a) = Fish displayed dark colouration
(b) = Fish were lethargic
(c) = Fish displayed a partial loss of equilibrium
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 96 hr LC50 of hexachlorocyclopentadiene for Lepomis macrochirus was 0.13 mg/L.
- Executive summary:
The short term toxicity of hexachlorocyclopentadiene to fish was determined using a procedure equivalent to the OECD Guideline for Testing of Chemicals 203 with acceptable restrictions. The study was carried out on Bluegill (Lepomis macrochirus). The study determined the percent mortality of the test species to the registered substance over a range of concentrations between 0.065 and 0.32 mg/L. It was determined that the 96 hour LC50 of the registered substance was 0.13 mg/L.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1977
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- yes
- Remarks:
- Data on light was not recorded
- GLP compliance:
- no
- Specific details on test material used for the study:
- TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: test compound dissolved in acetone - Analytical monitoring:
- not specified
- Details on sampling:
- - Concentrations: 0.32 mg/L; 0.24 mg/L; 0.18 mg/L; 0.16 mg/L; 0.14 mg/L; 0.12 mg/L; 0.087 mg/L.
- Vehicle:
- yes
- Remarks:
- acetone
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test compound for each concentration, dissolved in acetone, was added directly into each jar and mixed by stirring.
- Controls: Negative controls (same dilution water and conditions but with no test substance or acetone) and positive controls (containing greatest amount of acetone introduced into any one test vessel).
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Acetone - Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- TEST ORGANISM
- Common name: Fathead minnow
- Source: commercial fish hatchery in Arkansas
- Length at study initiation (length definition, mean, range and SD): 38 ± 5.2 mm
- Weight at study initiation (mean and range, SD): 0.72 ± 0.3 g
- Maintenance of the brood fish: fish were held in 1700L concrete raceways, coated with an epoxy resin paint to prevent leaching of extraneous materials into the water. Well water with a hardness flowed through these raceways at a minimum of 4 L / minute, providing an adequate water turn-over rate for maintaining the species.
ACCLIMATION
- Acclimation period: 48 hours
- Acclimation conditions (same as test or not): test conditions of temperature and water quality
- Feeding frequency during acclimation: fish were not fed during acclimation period
- Health during acclimation (any mortality observed): no mortality observed and fish appeared to be in excellent condition at the initiation of the tests
QUARANTINE
- Duration: 30 days
- Health/mortality: less than 2% - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 35 mg/L CaCO3
- Test temperature:
- 21 ± 1 °C
- pH:
- 7.1
- Dissolved oxygen:
- > 60% saturation
- Details on test conditions:
- TEST SYSTEM
- Test vessel: jars
- Material, size, headspace, fill volume: 19.6 L glass jars containing 15 L test solution
- Aeration: Test solutions were not aerated during the tests
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Intervals of water quality measurement: Dissolved oxygen concentration, pH and temperature of test solutions were checked at 0, 24, 48 and 96 hours of exposure in the highest, middle and lowest test concentrations, at a minimum.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : percentage mortality at 24, 48, and 96 hours
TEST CONCENTRATIONS
- Range finding study: The preliminary test defined a narrower range of concentrations to be used in the definitive test which would then assess the dose response relationship and permit calculation of LC50 and its 95% confidence interval from the mortality data produced.
- Test concentrations: 0.32 mg/L; 0.24 mg/L; 0.18 mg/L; 0.16 mg/L; 0.14 mg/L; 0.12 mg/L; 0.087 mg/L. - Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.24 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.21 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.18 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.087 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Other biological observations: The mortality syndrome among fish from those concentrations in which mortality was observed was similar. Fish generally became dark and lethargic, lost equilibrium, and expired.
- Mortality of control: no mortality
- Other adverse effects control: no effects - Reported statistics and error estimates:
- LC50 (24hr) = 0.17 - 0.32 mg/L (95% confidence interval)
LC50 (48hr) = 0.18 - 0.25 mg/L (95% confidence interval)
LC50 (96hr) = 0.16 - 0.22 mg/L (95% confidence interval) - Sublethal observations / clinical signs:
Table showing concentrations tested and corresponding observed percentage mortalities for fathead minnow (Pimephales promelas).
Nominal concentration (mg/L) % mortality observed 24 h 48 h 96 h 0.32 100 100 100 0.24 50 (a,b,c) 80 (b,c) 90 (a,b) 0.18 0 20 (a) 20 (a) 0.16 0 0 20 (a) 0.14 0 0 0 0.12 0 0 10 0.087 0 0 0 control (acetone) 0 0 0 control 0 0 0 (a) = Fish displayed dark colouration
(b) = Fish were lethargic
(c) = Fish displayed a partial loss of equilibrium
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 96 hr LC50 of hexachlorocyclopentadiene for Pimephales promelas was 0.18 mg/L.
- Executive summary:
The short term toxicity of hexachlorocyclopentadiene to fish (Pimephales promelas) was determined using a procedure equivalent to the OECD Guideline for Testing of Chemicals 203, with acceptable restrictions. The study was carried out on Fathead minnow (Pimephales promelas). The study determined the percentage mortality of the test species to the registered substance over a range of concentrations between 0.087 and 0.32 mg/L. It was determined that the 96 hour LC50 of the registered substance was 0.13 mg/L.
Referenceopen allclose all
Description of key information
Two non-GLP short-term toxicity studies were performed according to a method similar to the OECD Testing Guideline 203 on Lepomis macrochirus and Pimephales promelas respectively. The lowest 96-hour LC50 (0.13 mg/l) for Lepomis macrochirus was selected as the key representative value as it represents a worst-case scenario.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 0.13 mg/L
Additional information
The short-term toxicity of the registered substance to marine fish was not investigated as part of this REACH Registration. It was not considered as relevant taking into account the uses of the substance.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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