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EC number: 238-620-0 | CAS number: 14576-08-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 18 June 2010 to 19 July 2010.
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- (1992)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 835.3110 (Ready Biodegradability)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- - Substance name: Orange Flower Ether
- CAS number: 14576-08-0 - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- Fresh activated sludge from a biological waste water treatment plant treating predominantly dornestic sewage (Bois-de-Bay, Satigny, Switzerland) was used. The sludge is collected in the morning, washed three times in the mineral medium (by centrifuging at 1000 g for 10 minutes, discarding the supernatant and resuspending in mineral medium) and kept aerobic until being used on the same day. The dry weight of suspended solids is determined by taking two 50 mL samples of the homogenised sludge, evaporating water on a steam bath, drying in an oven at 105 - 110 °C for two hours and weighing the residue. The dry weight of suspended solid was 5.23 g/L and diluted to 1.0 g/L for dosage.
- Duration of test (contact time):
- 31 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- MINERAL MEDIUM
Prepared by mixing 50 mL of sofution A and 2000 mL deionised water, adding 5 mL of each of the solutions B, C and D and making up to 5 litres with deionised water. The pH is measured and if necessary adjusted to 7.4 ± 0.2 with phosphoric acid or potassium hydroxide.
- Solution A: 8.5 g/L KH2PO4, 21.75 g/L K2HPO4, 33.4 g/L Na2HPO4·2H20 and 0.5 g/L NH4Cl.
- Solution B: 27.5 g/L CaCl2
- Solution C: 22.5 g/L MgSO4·7H2O
- Solution D: 0.25 g/L FeCl3·6H2O and one drop of HCl per litre.
PREPARATION OF TEST SOLUTIONS
To obtain a concentration of 30 mg/L (dry weight) in 100/101 mL of test medium, 3.00 mL of diluted sludge is needed (inoculum). To obtain a concentration of 30 mg/L (dry weight) in 200 mL of test medium, 6.00 mL of diluted sludge is needed (inoculum).
PREPARATION OF TEST FLASKS
Test substance samples (10.0 mg, corresponding to 100 mg/L in 100 mL of test medium) are weighed in small aluminium boats and added directly to the test flasks of the Oxitop, whereas reference the substance (sodium benzoate) is added as 1.00 mL of a 10.0 g/L solution in mineral medium, to give a total volume of 101 mL. Flasks are filled with 97 mL of mineral medium (flasks containing sludge only: 194 mL). Samples of test or reference substance are added. Then suspended sludge diluted to a concentration of 1.0 g/L dry matter is added. Except when the test substance has an acid or alkaline character, the pH of each flask is not measured but assumed to be the same as the mineral medium, in order not to remove any floating undissolved test substance from the test medium by dipping a glass electrode in it. Neutral test substances, even sodium benzoate, were shown not to affect the pH of the medium by more than 0.1 pH unit. Two sodium hydroxide pellets are placed in the quivers on top of the bottle, and the flasks are closed tightly with the measuring heads. The flasks are allowed to equilibrate to the test temperature. The measurement is started by programming the measuring unit of the Oxitop test flasks, and the test flasks are placed in the temperature controlled cupboard of the Oxitop system. After temperature equilibration, the controller of the instrument starts the data acquisition (time zero of the experiment). The initial pH is set at 7.6. The test temperature is 22°C.
PERFORMANCE OF THE TEST
Everyday the oxygen consumption of each flask is recorded and correct temperature and stirring are checked. At the end of the test period (normally 28 days), the pH of each flask is measured again.
APPARATUS
The respirometer used during this study is an Oxitop Control System, made by Wissenschaftlich-Technische Werkstätten (WTW), Weilheim, Germany. - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 100 mg/L
- Test performance:
- - Degradation of sodium benzoate exceeded 40 % after 7 days and 65 % after 14 days: the activity of the inoculum was thus verified (validity criterion).
- Not more than 20% difference between replicates (validity criterion ).
- Orange Flower Ether did not inhibit the intrinsic respiration of the inoculum at the test concentration and was therefore considered to be non-toxic to the inoculum at the test concentration. - Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 76
- Sampling time:
- 28 d
- Remarks on result:
- other: The substance is considered readily biodegradable because > 70% is degraded within 28-days, despite the 10-day window criterion is not fulfilled (13 % biodegradation on day 9 and 59 % on day 19).
- Results with reference substance:
- 40 % after 7 days and 65 % after 14 days
- Validity criteria fulfilled:
- yes
- Remarks:
- See 'Test performance'.
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The substance is readily biodegradable.
- Executive summary:
The biodegradation potential of the substance in water was determined in a screening study according to OECD TG 301F (Manometric Respirometry) and in compliance with GLP criteria (Givaudan, 2010). In this study 100 mg/L test substance was inoculated with activated sludge from a municipal wastewater treatment plant for 31 days under aerobic conditions. During the incubation period the biological oxygen demand (BOD) was measured and biodegradation expressed as percentage of the theoretical uptake (ThOD). Orange Flower Ether did not inhibit the intrinsic respiration of the inoculum at the test concentration and was therefore considered to be non-toxic to the inoculum at the test concentration. The substance was biodegraded 76 % after 28 days in the test conditions. The 10-day window criterion was not fulfilled (13 % biodegradation on day 9 and 59 % on day 19). In view of the substance being degraded for > 70% (76%) and it contains more than one constituents the10-day window does not need to be met (CLP 4.1.2.9.5 consolidated version of Feb 2016: 4.1.2.9.5 at ii) c; ii), respectively). Based on these findings the substance is assessed as readily biodegradable.
Reference
Biodegradability
Test Flask
|
# |
Days |
||||||
8 |
9 |
14 |
19 |
21 |
28 |
31 |
||
O2 uptake of sludge (inoculum blank) |
1a |
22.7 |
22.7 |
28.9 |
33.0 |
35.1 |
39.2 |
41.3 |
2a |
22.7 |
24.8 |
31.0 |
37.1 |
37.1 |
43.3 |
47.5 |
|
Mean |
22.7 |
23.8 |
30.0 |
35.1 |
36.1 |
41.3 |
44.4 |
|
O2 uptake of test substance + sludge |
17a |
37.7 |
53.9 |
150.9 |
198.8 |
215.8 |
252.8 |
263.8 |
18a |
48.5 |
70.1 |
167.1 |
221.2 |
237.2 |
280.2 |
286.2 |
|
O2 uptake of test substance |
|
15.0 |
30.1 |
120.9 |
163.8 |
179.7 |
211.5 |
219.4 |
|
25.8 |
46.4 |
137.2 |
186.1 |
201.1 |
239.0 |
241.8 |
|
% biodegradation of test substance |
|
5 |
10 |
41 |
56 |
61 |
72 |
74 |
|
9 |
16 |
47 |
63 |
68 |
81 |
82 |
|
mean |
7 |
13 |
44 |
59 |
65 |
76 |
78 |
Oxygen uptakes, as read on the Oxitop controller, are corrected to account for the small differences between actual and nominal concentrations of test and reference substances.
Description of key information
The biodegradation potential of the substance in water was determined in a screening study according to OECD TG 301F. Under the conditions of the test the substance was readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
The biodegradation potential of the substance in water was determined in a screening study according to OECD TG 301F (Manometric Respirometry) and in compliance with GLP criteria (Givaudan, 2010). In this study 100 mg/L test substance was inoculated with activated sludge from a municipal wastewater treatment plant for 31 days under aerobic conditions. During the incubation period the biological oxygen demand (BOD) was measured and biodegradation expressed as percentage of the theoretical uptake (ThOD). Orange Flower Ether did not inhibit the intrinsic respiration of the inoculum at the test concentration and was therefore considered to be non-toxic to the inoculum at the test concentration. The substance was biodegraded 76 % after 28 days in the test conditions. The 10-day window criterion was not fulfilled (13 % biodegradation on day 9 and 59 % on day 19). In view of the substance being degraded for > 70% (76%) and it contains more than one constituents the10-day window does not need to be met (CLP 4.1.2.9.5 consolidated version of Feb 2016: 4.1.2.9.5 at ii) c; ii), respectively). Based on these findings the substance is assessed as readily biodegradable.
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