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EC number: 202-905-8 | CAS number: 100-97-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1995
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: non GLP, no guideline
- Qualifier:
- according to guideline
- Guideline:
- DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
- Deviations:
- yes
- Remarks:
- additional time points 60 and 90 min
- Principles of method if other than guideline:
- Measurement of luminescence
- GLP compliance:
- not specified
- Analytical monitoring:
- no
- Details on sampling:
- no sampling
- Vehicle:
- no
- Details on test solutions:
- no details given
- Test organisms (species):
- Vibrio fisheri
- Details on inoculum:
- The marine, luminescent bacteria strain Vibrio fischeri NRRL-B-11177 (formerly Photobacterium phosphoreum; DSM 7151) was used for luminescence inhibition test system. In order to ensure optimal salt conditions for the bacteria, the samples must have a salt content of 2%, which was achieved by adding 2% NaCl in solid form.
- Test type:
- static
- Water media type:
- saltwater
- Limit test:
- no
- Total exposure duration:
- 30 min
- Remarks on exposure duration:
- 60 and 90 min also tested
- Post exposure observation period:
- no post exposure period
- Hardness:
- not indicated
- Test temperature:
- 15°C
- pH:
- not indicated
- Dissolved oxygen:
- not indicated
- Salinity:
- 2 %
- Nominal and measured concentrations:
- 5000 mg/L (nominal)
- Details on test conditions:
- The LUMIStox measuring unit from Dr. Lange GmbH (Berlin, Germany) was used to determine the ecotoxicological potential of Hexamethylenetetramine. The EC50 values were determined. The sample sequence corresponds to DIN 38412 L34 (L341), which ranges from 1:2 to 1:32-dilution of the compound in the test system. All dilution steps were made in duplicate. The change of intensity of bacterial luminescence in the absence (controls) and in the presence (samples) of Hexamethylenetetramine after different incubation times (30 min according to DIN 38412 and after 60 and 90 min) were recorded. The controls (with 2% NaCl only) were measured for calculating the correction factor, which was necessary to consider the "normal" decrease of luminescence (without any toxic effect) per time. The corrected values were used for calculating the percentage of inhibition of luminescence per incubation period of each sample. The bioluminescence of the bacteria is measured by a temperature-controlled photomultipier at 15°C.
- Reference substance (positive control):
- yes
- Remarks:
- other substances with positive effects were measured in parallel.
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 5 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: luminescence
- Key result
- Duration:
- 60 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 5 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: luminescence
- Key result
- Duration:
- 90 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 5 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: luminescence
- Details on results:
- no details given
- Results with reference substance (positive control):
- Other substances with positive effects were measured in parallel.
- Reported statistics and error estimates:
- not indicated
- Validity criteria fulfilled:
- yes
- Conclusions:
- The LC50 of methenamine in Vibiro fischeri was determined to be > 5000 mg/L.
- Executive summary:
In a 30, 60 and 90 min toxicity study, cultures of Vibrio fischeri were exposed to methenamine at a nominal limit-concentrations of 5000 mg/L under static conditions. The NOEC and EC50 values based are based on luminescence. 5000 mg/l, the highest concentration tested, did not reveal any effects for a test duration of up to 90 min. Thus the EC50 and the NOEC values were > 5000 mg/L.
This toxicity study is classified as acceptable for the risk assessment for STPs.
Results Synopsis
Test Organism: Vibrio fischeri
Test Type: Static
90 min EC50: > 5000 mg/L
90 min NOEC: >= 5000 mg/L
- Endpoint:
- activated sludge nitrification inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1977
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: non GLP, no guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Measurement of inhibition of nitrification.
- GLP compliance:
- not specified
- Analytical monitoring:
- no
- Details on sampling:
- no sampling
- Vehicle:
- no
- Details on test solutions:
- not indicated
- Test organisms (species):
- other: Nitrosomas sp. and Nitrobacter sp.
- Details on inoculum:
- autotrophic nitrifying bacteria
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 2 h
- Post exposure observation period:
- no post exposure period
- Hardness:
- not indicated
- Test temperature:
- not indicated
- pH:
- 8.1
- Dissolved oxygen:
- not indicated
- Salinity:
- not indicated
- Nominal and measured concentrations:
- nominal: 100 mg/L
- Details on test conditions:
- Throughout the experiments a stock solution of autotrophic nitrifying bacteria was maintained under continuous culture conditions. Organisms were removed from this culture and used in a standard assay to determine the effects of the Hexamethylenetetramine by comparing the rate of nitrate production in the presence of the test compound to the rate in its absence. All determinations were made in duplicate, and Hexamethylenetetramine was considered to be inhibitory if it reduced the rate by more than 10 %.
- Reference substance (positive control):
- yes
- Remarks:
- other substances with positive response were tested in parallel.
- Key result
- Duration:
- 2 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of nitrification rate
- Key result
- Duration:
- 2 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of nitrification rate
- Details on results:
- 100 mg/l, the highest concentration tested, did not reveal any effects for a test duration of 2 hours.
- Results with reference substance (positive control):
- Other substances with positive response were tested in parallel.
- Reported statistics and error estimates:
- not indicated
- Validity criteria fulfilled:
- yes
- Conclusions:
- The LC50 of methenamine in nitrifying bacteria (Nitrosomas sp. and Nitrobacter sp.) was determined to be > 100 mg/L.
- Executive summary:
In a 2 hour acute toxicity study, cultures of nitrifying bacteria (Nitrosomas sp. and Nitrobacter sp.) were exposed to methenamine at a nominal limit-concentrations of 100 mg/L under static conditions. The NOEC and EC50 values based are based on nitrification (production of nitrate). 100 mg/l, the highest concentration tested, did not reveal any effects for a test duration of 2 hours. Thus the EC50 and the NOEC values were > 100 mg/L.
This toxicity study is classified as acceptable for the risk assessment.
Results Synopsis
Test Organism: nitrifying bacteria (Nitrosomas sp. and Nitrobacter sp.)
Test Type: Static
2 hr EC50: > 100 mg/L
2 hr NOEC: >= 100 mg/L
Referenceopen allclose all
Description of key information
The LC50 of methenamine in nitrifying bacteria (Nitrosomas sp. and Nitrobacter sp.) was determined to be > 100 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 100 mg/L
- EC10 or NOEC for microorganisms:
- 100 mg/L
Additional information
Key studies:
- Freshwater: In a 2 hour acute toxicity study (Hockenbury, 1977), cultures of nitrifying bacteria (Nitrosomas sp. and Nitrobacter sp.) were exposed to methenamine at a nominal limit-concentration of 100 mg/L under static conditions. The NOEC and EC50 values are based on nitrification (production of nitrate). 100 mg/L, the highest concentration tested, did not reveal any effects for a test duration of 2 hours. Thus the EC50 and the NOEC values were > 100 mg/L.
This toxicity study is classified as acceptable for the risk assessment.
- Saltwater: In a 30, 60 and 90 min toxicity study (Drzyzga, 1995), cultures of Vibrio fischeri were exposed to methenamine at a nominal limit-concentrations of 5000 mg/L under static conditions. The NOEC and EC50 values based are based on luminescence. 5000 mg/l, the highest concentration tested, did not reveal any effects for a test duration of up to 90 min. Thus the EC50 and the NOEC values were > 5000 mg/L.
This toxicity study is classified as acceptable for the risk assessment.
Supporting study:
- In a non standard assay (Greenwood, 1981) the inhibitory effect of bacteria growth of methenamine was examined. Different strains of Escherichia coli and Klebsiella aerogenes showed MAC-values (minimum antibacterial concentration = lowest concentration which caused partial inhibition of growth) between 32 and 125 mg/L and MIC-values (minimum inhibitory concentration = lowest concentration which inhibited growth overnight) between 250 and 500 mg/L. No NOECs or EC50 values are given or can be calculated, based on the data presented. Thus this study cannot be used for risk assessment purposes.
Based on the available data the NOEC > 100 mg/L observed in Nitrosomas sp. and Nitrobacter sp. can be considered as a conservative reference figure for the risk assessment of STPs.
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