Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 277-552-6 | CAS number: 73612-29-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- The test item concentration in the test solution was measured at the start and end of exposure. At the start, the solution sampled from the container for preparation was used for the measurement. At the end of exposure, equal volume of the test solution was taken out from test vessels in each exposure level, mixed, and used for the measurement.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
In a preliminary study the solubility in the test item was measured by using different loading concentrations: The test sample was added in medium to produce the nominal loading concentrations of 10 and 1 mg/L. After that, they were stirred for 24 and 48 hours under 23±1 °C and settled, and then the middle layer was collected. The middle layer was filtered through a glass fiber filter(No absorption to the filter was checked) (GB-140, pore size: 0.4 µm) and centrifuged (10,000xg, 30 min.) to remove the insoluble material. Then, the concentration of the test item was measured by HPLC (n=2).
The result suggested !hat the solubility of the test item in medium ranged from 0.6 to 1 mg/L. lt is only necessary to select nominal concentration which is higher than the predicted value of solubility. Therfore, a nominal loading rate of 2 mg/L was used for the actual test:
Correction with the purity was not applied to the preparatiou of the test solution.
Dispersed suspension of tbe test item (nominal loading concentration: 2 mg/L) was produced by stirring for about 48 hours, and then filtered to prepare the filtrate. Five test concentrations of 100, 55.6, 30.9, 17.1 and 9.53% as filtrate content (a geometric series with a factor of 1.8) and a control were selected. - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Strain: ATCC 22662
- Source (laboratory, culture collection): Originally from the American Type Culture Collection (12301 Parklawn Drive Rockville, Maryland 20852-1776 U.S.A.) on June 30, 1995 and have been cultured in the Kururne Labaratory
- Age of inoculum (at test initiation): The pre-culture, incubated under the same conditions as the test for 3 days and exponentially growing was used as inoculum to prepare the initial cell concentration of approximately 104 cells/mL. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 21 - 24 °C
- pH:
- 7.8 - 8
- Nominal and measured concentrations:
- - Nominal: 2 mg/L ( = 100 %); 55.6%, 30.9%, 17.1%, 8.53%
- Measured: 0.941, 0.531, 0.294, 0.177, 0.0982 mg/L; concentrations were based on a range-finding study - Details on test conditions:
- TEST SYSTEM
- Test vessel: sterilized 500 mL Erlenmeyer flask (with gas-permeable Silicosen®)
- Volume of test solution: 600 mL/controllevel (100 mL/vessel); 300 mL/exposure Ievel (100 mL/vessel)
- Initial cells density: the pre-culture, incubated under the same conditions as the lest for 3 days and exponentially growing was used as inoculum to prepare the initial cell concentration of approximately 10E4 cells/mL.
- No. of vessels per concentration: 3
- No. of vessels per control: 6
GROWTH MEDIUM
- Standard medium used: yes (medium recommended in OECD test guideline 201)
TEST MEDIUM / WATER PARAMETERS
- Intervals of water quality measurement: the pH of the test solution was measured at the start and end of exposure. For the measurement of pH, another solution sampled from the container for preparation was used at the start of exposure and one vessel in each test level was used for the measurement at the end of exposure. The temperature and light intensity in the incubator were measured once a day during exposure.
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: No
- Light intensity and quality: Continuous illumination provided with 60 - 120 µE·mE-2·sE-1 at the level of the test solutions, not varied more than 20% using a fluorescent light with wavelength range of 400 - 700 nm.
EFFECT PARAMETERS MEASURED:
- Determination of cell concentrations: Cell concentration was counted with particle counter (Model COULTER Z1, Beckman Coulter) at 24, 48 and 72 hours after the start of exposure
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- Range finding study: In the preliminary studies, excessive amount of the test sample was added in medium (nominal loading concentration: 2 mg/L) and stirred for about 48 hours. After that, insoluble material was removed to produce the saturated solution which was at around the predicted solubility (1 mg/L). Then, the test organisms were · exposed to the saturated solution. As a result, the growth inhibition below 50% was observed in the saturated solution. In addition, no growth inhibition was observed in the 9.53% content solution of the saturated solution. Therefore, five test concentrations described above were selected. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.941 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: no effects observed up to the limit of water solubility
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 0.941 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.941 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.531 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- No acute toxic effects within the range of solubility.
- Results with reference substance (positive control):
- An algae growth inhibition test of potassium dichromate (Reagent grade, Wako Pure Chemical Industries) with the test organisms was conducted to confirm the reproducibility of the test system (August 29 - September 1, 2005). The EbC50 (0 - 72h) and ErC50 (0 - 3d) of potassium dichromate were 0.492 and 1.09 mg/L respectively [Background data : Mean ± S.D. of EbC50 ( 0-72h ) and ErC50 (0 - 3d) was 0.408 ± 0.079 mg/L and 1.12 ± 0.44 mg/L (n=10), respectively (including extrapolated value of ErC50s (0 - 3d)].
- Validity criteria fulfilled:
- yes
- Conclusions:
- With high probability acutely not harmful to aquatic algae.
Reference
Description of key information
With high probability acutely not harmful to aquatic algae.
Key value for chemical safety assessment
Additional information
A study according to GLP and OECD 201 was conducted to determine the effect of the test item on the growth of the aquatic algae Pseudokirchneriella subcapitata (Kurume Laboratory 2005). After 72 h no effects were observed up to the limit of water solubility (at a nominal loading rate of 2 mg/L).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.