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EC number: 238-056-5 | CAS number: 14205-39-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 986
- Report date:
- 1986
Materials and methods
- Principles of method if other than guideline:
- The point-mutagenic effects were investigated in doses up to 12000 µg/plate on four histidine-auxotropic Salmonella typhimurium LT2 strains, for each substance, dose level and strain 4 plates were used. After an incubation period of 48 h the number of revertant colonies per plate were counted.
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Methyl 3-aminocrotonate
- EC Number:
- 238-056-5
- EC Name:
- Methyl 3-aminocrotonate
- Cas Number:
- 14205-39-1
- Molecular formula:
- C5 H9 O2 N
- IUPAC Name:
- methyl 3-aminocrotonate
- Details on test material:
- 3-Aminocrotonsäuremethylester, white powder, content and identity not certified
Constituent 1
Method
- Target gene:
- histidin gene locus
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA 1535, TA 100, TA 1537, TA 98
- Metabolic activation:
- with and without
- Metabolic activation system:
- rat liver homogenate (S-9 mix)
- Test concentrations with justification for top dose:
- 3-Aminocrotonsäuremethylester: 375-12000 µg/plate
Sodium azide: 10 µg/plate
Nitrofurantoin: 0.2 µg/plate
4-Nitro-o-phenylendiamin: 10 µg/plate (TA 1537) and 0.5 µg/plate (TA 98)
2-Aminoanthracene: 3 µg/plate
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Ethanol
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: Sodium azide (TA 1535, without S9-mix), Nitrofurantoin (TA 100, without S9-mix), 4-Nitro-o-phenylendiamin (TA 1537 and 98, without S9-mix), 2-Aminoanthracene (with S9-mix)
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: TA 1535, TA 100, TA 1537, TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- at doses of 1500 µg/plate and higher bacteriotoxic effects, but these were only weak strainspecific effects, so that this range could nevertheless be used for evaluation purposes
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Executive summary:
Methyl 3 -aminocrotonate was tested in the Salmonella/microsome test for point-mutagenic effects in doses up to 12000 µg/plate on the four histidine-auxotrophic Salmonella typhimurium LT2 strains TA 1535, TA 100, TA 1537 and TA 98.
Doses up to and including 750 µg/plate did not cause any bacteriotoxic effects and at higher doses the substance had only a weak strainspecific bacteriotoxic effect, so that this range could nevertheless be used for evaluation purposes.
No evidence for a mutagenic activity of methyl 3 -aminocrotonate was found.
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