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EC number: 220-912-4 | CAS number: 2935-90-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- N/A to 1983-03-08
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study conducted with methods similar to OECD guideline 471. However, an E. coli strain or S. typhimurium strain TA102 was not included.
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 983
- Report date:
- 1983
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- An E. coli strain or S. typhimurium strain TA102 was not included.
- Principles of method if other than guideline:
- N/A
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Methyl 3-mercaptopropionate
- EC Number:
- 220-912-4
- EC Name:
- Methyl 3-mercaptopropionate
- Cas Number:
- 2935-90-2
- Molecular formula:
- C4H8O2S
- IUPAC Name:
- methyl 3-sulfanylpropanoate
- Details on test material:
- - Name of test material (as cited in study report): methyl 3-mercaptopropionate
- Molecular formula (if other than submission substance): N/A
- Molecular weight (if other than submission substance): N/A
- Smiles notation (if other than submission substance): N/A
- InChl (if other than submission substance): N/A
- Structural formula attached as image file (if other than submission substance): N/A
- Substance type: active
- Physical state: liquid
Constituent 1
Method
- Target gene:
- histidine locus
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA1538, TA1537, TA1535, TA98 and TA100
- Details on mammalian cell type (if applicable):
- N/A
- Additional strain / cell type characteristics:
- other: See below for additional strain characteristics.
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254 induced rat liver S9
- Test concentrations with justification for top dose:
- 61.7, 185.2, 555.6, 1666.7 and 5000 ug/plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: The test substance was freely soluble in DMSO.
Controlsopen allclose all
- Untreated negative controls:
- yes
- Remarks:
- untreated
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO (vehicle of the test substance)
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: Methylnitronitrosoguanidine- used for TA1535 and TA100 at 5 ug/plate
- Remarks:
- without metabolic activation
- Untreated negative controls:
- yes
- Remarks:
- untreated
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO (vehicle of the test substance)
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene-used for all strains at 5 ug/plate
- Remarks:
- with metabolic activation
- Untreated negative controls:
- yes
- Remarks:
- untreated
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO (vehicle of the test substance)
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- without metabolic activation
Migrated to IUCLID6: used for TA1537 at 75 ug/plate
- Untreated negative controls:
- yes
- Remarks:
- untreated
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO (vehicle of the test substance)
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- Remarks:
- without metabolic activation
Migrated to IUCLID6: used for TA1538 and TA98 at 50 ug/plate
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Preincubation period: N/A
- Exposure duration: ~ 2 days (Contents were poured onto VBE minimal agar plates, gently rotated and tilted to assure uniform distribution of the top agar, allowed to harden on an even surface for one hour, and inverted and put in a dark 37 +/- 0.5 deg. C incubator.)
- Expression time (cells in growth medium): N/A
- Selection time (if incubation with a selection agent): N/A
- Fixation time (start of exposure up to fixation or harvest of cells): N/A
SELECTION AGENT (mutation assays): N/A
SPINDLE INHIBITOR (cytogenetic assays): N/A
STAIN (for cytogenetic assays): N/A
NUMBER OF REPLICATIONS: 3
NUMBER OF CELLS EVALUATED: N/A
DETERMINATION OF CYTOTOXICITY
- Method: measurement of the bacterial background lawn
OTHER EXAMINATIONS:
- Determination of polyploidy: N/A
- Determination of endoreplication: N/A
- Other: N/A
OTHER:
Spontaneous revertant frequencies were determined and had to be within acceptable limits for each study. The presence of the specific genetic markers were determined periodically for each tester strain. Each organism was routinely checked for: 1) histidine requirement, 2) biotin requirement (for uvrB deletion), 3) sensitivity to crystal violet and/or deoxycholate (for deep rough (rfa) mutation to cell wall), and 4) ampicillin resistant R factor (for tester strains TA100 and TA98). Each new batch of S9 was checked with standard mutagens to evaluate its strength and to find the optimum amount to use for general screening. - Evaluation criteria:
- For the test to be considered valid, the following conditions were required:
1: Demonstration of toxicity of the test substance for the tester strains, unless this was not possible due to limited solubility of the test substance.
2: The negative control responses were within the normal range of the laboratory database.
3. Confirmation of sensitivity and responsiveness of the tester strains to mutagenic action as indicated by their responses to the positive controls.
If the above criteria were met, the test substance was considered to be mutagenic if it induced a positive response in a dose-related manner over three concentrations, with the baseline increase in the number of histidine revertants equal to twice the solvent control level. - Statistics:
- N/A
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: TA1538, TA1537, TA1535, TA98 and TA100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Effects of pH: N/A
- Effects of osmolality: N/A
- Evaporation from medium: N/A
- Water solubility: N/A
- Precipitation: N/A
- Other confounding effects: N/A
RANGE-FINDING/SCREENING STUDIES:
Dose levels for the mutagenicity test were determined in a range-finding (toxicity) study. 2 mL of complete top agar, 0.1 mL of an overnight culture of TA100 and 0.1 mL of various concentrations of the test substance in the absence of metabolic activation were combined. The contents of the tube were thoroughly mixed, poured and uniformly distributed over VBE minimal agar. The plates were allowed to harden approximately one hour, inverted and put into a dark 37+/-0.5 degrees C incubator. After two days, the background lawn of growth and revertant colonies in both test and control plates were scored. Observations of test substance precipitation were similarly recorded. The maximum dose selected for the mutagenicity test was 5,000 ug/plate because it exhibited growth inhibition. No further details on concentrations used or results of the range-finding study were provided.
COMPARISON WITH HISTORICAL CONTROL DATA: N/A
ADDITIONAL INFORMATION ON CYTOTOXICITY:
The test substance was cytotoxic at 5000 ug/plate in both the presence and absence of metabolic activation in all strains, except in strain TA98 in the presence of metabolic activation. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
The test substance in the presence and absence of metabolic activation did not increase the reversion rate of any of the strains, and was therefore considered not to be mutagenic in the test system.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative with and without metabolic activation
The test substance was evaluated for mutagenic potential in the absence and presence of metabolic activation using the Ames test. Under the conditions of the study, the test substance was determined to be non-mutagenic up to a dose level of 5000 ug/plate. - Executive summary:
N/A
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