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EC number: 203-841-3 | CAS number: 111-17-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study with acceptable restrictions. Only translated summary available, actual guideline not stated.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Report date:
- 2003
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- Only summary available, guideline not stated
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 3,3'-thiodi(propionic acid)
- EC Number:
- 203-841-3
- EC Name:
- 3,3'-thiodi(propionic acid)
- Cas Number:
- 111-17-1
- Molecular formula:
- C6H10O4S
- IUPAC Name:
- 3-[(2-carboxyethyl)sulfanyl]propanoic acid
- Details on test material:
- - Name of test material (as cited in study report): Thiodipropionic acid (TDPA), 3,3’-thiodipropionic acid; bis(2-carboxyethyl) sulfide; ß,ß-thiodipropionic acid; 3,3’-thiobis(propanoic acid); 4-thiaheptanedioic acid; diethyl sulfide 2,2’-dicarboxylic acid; thiodihydracrylic acid
- Analytical purity: no data
Constituent 1
Method
- Target gene:
- Histidine operon for TA100, TA98,TA1535, TA1537, tryptophan operon for E. coli WP2 uvrA
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-mix
- Test concentrations with justification for top dose:
- 156, 313, 625, 1250, 2500, 5000µg/mL
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- benzo(a)pyrene
- Remarks:
- with S9-mix Migrated to IUCLID6: 5µg/plate for TA 98, TA 100, TA 1537
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-Aminanthrazene, 2µg/plate for TA 1535 and 10µg/plate for E. coli WP2 uvrA
- Remarks:
- with S9-mix
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-(2-furyl)-3-(5-nitro-2-furyl) acrylamide, 0.01µg/plate for E. coli WP2 uvrA and TA 100 and 0.1µg/plate for TA 98
- Remarks:
- without S9-mix
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- without S9-mix Migrated to IUCLID6: 0.5µg/plate for TA 1535
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-methoxy-6-chloro-9-[3-(2-chloroethyl)-aminopropylamino] acridine·2HCl, 1µg/plate for TA 1537
- Remarks:
- without S9-mix
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in medium; in agar (plate incorporation) with preincubation
DURATION
- Preincubation period: 20min
- Exposure duration: 48h
NUMBER OF REPLICATIONS: 3
NUMBER OF CELLS EVALUATED: no data
DETERMINATION OF CYTOTOXICITY
- Method: relative total growth by colonie count - Evaluation criteria:
- The test was determined to be positive if the number of revertant colonies was nearly double or above that of the solvent control and this was found to be reproducible and to be dependant on the dosage of the test material.
- Statistics:
- Statistical methods were not used
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation: no
RANGE-FINDING/SCREENING STUDIES:
The results of the dose-range finding test and main test did not find growth inhibition or deposition of precipitation/crystals in regards to the test strains at any concentration regardless of the strain type or whether or not there was metabolic activation.
Any other information on results incl. tables
Table 1: Maximum revertant counts
|
Maximum number of revertants with |
|||||
solvent Control |
positive control |
Treatment (at dose level [µg/mL]) |
||||
Strain |
With S9 |
Without S9 |
With S9 |
Without S9 |
With S9 |
Without S9 |
TA 100 |
126 |
106 |
529 |
884 |
120 (2500) |
118 (625) |
TA 1535 |
8 |
13 |
91 |
1329 |
11 (313) |
12 (313) |
E. coli WP2 |
26 |
31 |
180 |
635 |
33 (313) |
29 (1250) |
TA 98 |
25 |
15 |
318 |
603 |
28 (313) |
16 (1250) |
TA 1537 |
12 |
15 |
93 |
2480 |
18 (313) |
17 (625) |
From the above results, it was determined that under these test conditions 3,3’-thiobispropanoic acid does not induce genetic mutations on bacteria.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results: negative
- Executive summary:
In a bacterial reverse mutation assay equivalent to OECD TG 471 S. typhimurium TA 1535, TA 1537, TA 98 and TA 100 and E. coli WP2 uvr A were exposed to 3,3’-thiobispropanoic acid at concentrations of 0 (control), 156, 313, 625, 1250, 2500, 5000 µg/mL in DMSO. The test was performed as plate incoporation and pre-incubation assay. Cytotoxicity was not observed. The test item did not induce revertants above background. Thus, 3,3’-thiobispropanoic acid is not mutagenic in this assay.
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