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EC number: 203-841-3 | CAS number: 111-17-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Take a 0.75 mL sample of test liquid from each test container at the start of exposure and after 24 hours (before changing the water), and after acetonitrile was equally added and stirred into this, it was analyzed using HPLC.
The test material concentration of each test liquid was quantified from the ratio with the standard solution peak area to find the geometric mean. - Test organisms (species):
- Oryzias latipes
- Details on test organisms:
- TEST ORGANISM
- Common name: Himedaka (Japanese name)
- Source: Reared in-house
- Age at study initiation: Approximately 6 months from hatching
- Length at study initiation: Average 1.55 cm (1.50 to 1.86 cm), n = 10
- Weight at study initiation: Average 0.054 g (0.035 to 0.135 g), n = 10
- Feeding during test: no feeding from 24 hours before the start of exposure
ACCLIMATION
- Acclimation period: January 28, 2004 to April 5, 2004
- Type and amount of food: Approx. 2% of fish body weight/day
- Health during acclimation: The morality rate 7 days prior to the start of exposure was under 5%, and grossly healthy and normal specimens were used for the test - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 59 mg CaCO3/L
- Test temperature:
- 24±1ºC
- pH:
- The test liquid pH was not adjusted: Control 6.8-7.5; container with test substance: 4.7-4.9
- Dissolved oxygen:
- A saturated concentration of 60% or more was maintained (no aeration)
- Nominal and measured concentrations:
- Control + 100 mg/L (nominal)
median measured concentration: 99.1 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: closed (the water surface was covered with a lid made of Teflon sheet)
- Material, size, headspace, fill volume: 5 L glass container
- Aeration: No
- Renewal rate of test solution: Test liquid is completely replaced every 24 hours
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates):1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: For the dilution water, Yokohama City tap water was processed with activated charcoal to remove the residual chlorine, etc., and the water was sufficiently aerated, so dechlorinated tap water controlled at a temperature of 24 ± 2ºC was used. It was confirmed that there was not residual chlorine at the time of use. It was determined that the water quality was suitable as water for raising fish.
- Metals: lead: < 0.01 mg/L; mercury: < 0.0001 mg/L; Cadmium < 0.001 mg/L; Arsenic <0.005 mg/L; Chromium < 0.005 mg/L; Copper < 0.001 mg/L; Zinc 0.01 mg/L; Aluminium 0.05 mg/L; Tin <0.1 mg/L; Iron < 0.1 mg/L; Nickel <0.01 mg/L
- Chlorine: free chlorine < 0.02 mg/L
- Alkalinity: 44 mg/L
- Conductivity: 156 µS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: once a day
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours
- Light intensity: 1000 lux or less
EFFECT PARAMETERS MEASURED: mortality, abnormal mobility, abnormal breathing, immobility
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 10, 32, 100 mg/L - Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 99.1 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 99.1 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 99.1 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 99.1 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - One fish of the test substance group died within 24h (10% mortality) (see table 2)
- pH in group with test substance was with 4.7-4.9 very low over the whole test (see table 3)
- Behavioural abnormalities: No abnormal signs were observed in the surviving fish in the control group during the exposure period. Abnormal mobility (languid movement) was observed in the 100 mg/L concentration group.
- Mortality of control: none - Sublethal observations / clinical signs:
Table 1: Measured Concentrations of the Test Substance in Test Water (Semi-Static Condition)
Nominal Concentration (mg/L)
Measured Concentration (mg/L)
(Percent of Nominal)
Meana Measured Concentration (mg/L)
0 Hour (new)
24 Hours (old)
Control
< 0.08
< 0.08
----
100
103
(103)
95.3
(95)
99.1
(99)
Table 2: Mortality of the Medaka (Oryzias latipes) Exposed to the Test Substance
Nominal Concentration (mg/L)
Meana Measured Concentration (mg/L)
Cumulative Mortality (Percent Mortality)
24 Hours
48 Hours
72 Hours
96 Hours
Control
------
0 (0)
0 (0)
0 (0)
0 (0)
100
99.1
1 (10)
1 (10)
1 (10)
1 (10)
a: geometric mean
Table 3: pH Values
Nominal Concentration (mg/L)
MeanaMeasured Concentration (mg/L)
pH
0 Hour
24 Hours
48 Hours
72 Hours
96 Hours
new
old
new
old
new
old
new
old
Control
------
7.5
6.9
7.2
7.3
7.2
6.8
7.2
7.2
100
99.1
4.9
4.9
4.9
4.9
4.9
4.7
4.7
4.8
a: geometric mean
new: freshly prepared test solutions
old: test solutions after 24 hours exposure
Table 4: Temperature
Nominal Concentration (mg/L)
MeanaMeasured Concentration (mg/L)
Temperature (°C)
0 Hour
24 Hours
48 Hours
72 Hours
96 Hours
new
old
new
old
new
old
new
old
Control
------
23.4
23.7
23.7
23.8
23.8
23.7
23.8
23.8
100
99.1
23.3
23.8
23.3
23.8
23.4
23.8
23.5
23.7
a: geometric mean
new: freshly prepared test solutions
old: test solutions after 24 hours exposure
Table 5: Dissolved Oxygen Concentrations
Nominal Concentration (mg/L)
MeanaMeasured Concentration (mg/L)
Dissolved Oxygen Concentration (mg/L)
0 Hour
24 Hours
48 Hours
72 Hours
96 Hours
new
old
new
old
new
old
new
old
Control
------
8.3
7.3
8.5
7.5
8.3
7.5
8.4
7.4
100
99.1
8.3
7.1
8.5
7.5
8.4
7.6
8.5
7.6
a: geometric mean
new: freshly prepared test solutions
old: test solutions after 24 hours exposure
Reference
Description of key information
LC50 (96h) > 100 mg/L
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
The semi-static limit test with freshwater fish (Oryzias latipes) over 96 hours was conducted according to OECD guideline 203 and observed no mortality at the tested concentration of 100 mg/L. The LC50 (96h) is > 100 mg/L (nominal concentration) and > 99.1 mg/L based on the geometrical mean of measured concentrations. Thus, only a low toxicity of the test substance to fish is expected.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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