2-hydroxy-1-(4-(4-(2-hydroxy-2-methylpropionyl)benzyl)phenyl)-2-methylpropan-1-one

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2005-03-03 to 2005-03-10

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)

Version / remarks:

adopted on June 14, 1996

Deviations:

no

GLP compliance:

yes

Radiolabelling:

no

Details on sampling:

- Sampling intervals/frequency for test organisms: on day 7, 14, 21, 23, 28
- Sampling intervals/frequency for test medium samples: on day 7, 14, 21, 23, 28

Vehicle:

yes

Details on preparation of test solutions, spiked fish food or sediment:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was dissolved in DMSO to prepare 25000 mg/L test substance stock solution. The stock solution was diluted with the test water supply to be used for 96hr-LC50 test.
- Controls: yes
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): DMSO

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: common carp (cyprinus carpio)
- Source: Aqua stage 21 Tokyo shop (1-12-8, Ojihoncho, Kita-ku, Tokyo)
- Length at study initiation (lenght definition, mean, range and SD): 5.7 ± 0.3 cm
- Weight at study initiation (mean and range, SD): 2.03 ± 0.33 g
- Feeding during test: yes; no feeding a day before sampling
- Food type: Mixed carp pellets (Suimmy) (Crude protein 37.0% or greater; Crude fat 2.5% or greater)
- Amount: ca. 2% per body weight/day
- Frequency: daily

ACCLIMATION
- Acclimation period: 7 days
- Acclimation conditions (same as test or not): yes
- Health during acclimation (any mortality observed): no mortality was observed

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

28 d

Hardness:

43 mg/L

Test temperature:

23.7 ± 0.6°C

pH:

7.1 ±0.1

Dissolved oxygen:

7.2 + 0.2 mg/L

TOC:

< 2 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquarium volume 100 L (water volume 50 L)
- Aeration: yes
- Renewal rate of test solution: 300 mL/min (432 L/day)
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 4
- No. of vessels per control / vehicle control (replicates): 1


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: JIS K0102 33.1 (alpha-Tolidine colorimetry method).
- Metals: copper < 0.005 mg/L
- Chlorine: < 0.01 mg/L
- Holding medium different from test medium: no
- Intervals of water quality measurement: temperature, pH and DO : on day 0, 7, 14, 21, 23, 28

OTHER TEST CONDITIONS
- Photoperiod: The operations were performed in a dark place because of instability of the test substance against the light.


RANGE-FINDING / PRELIMINARY STUDY
- Test concentrations: The 96hr-LC50 value for zebra-fish {Danio rerio) was determined to be 1.95 mg/L. Based on this value and the quantifiable limit of this analytical method, the following test concentrations were selected by setting safety factors of 1/100 and 1/1000.

Nominal and measured concentrations:

- two nominal concentrations: 0.02 mg/L; 0.002 mg/L

Reference substance (positive control):

no

Details on estimation of bioconcentration:

BASIS INFORMATION
- Measured/calculated logPow: 2.3

Conc. / dose:

0.02 mg/L

Temp.:

23.7 °C

pH:

7.1

Type:

BCF

Value:

< 1 dimensionless

Basis:

not specified

Time of plateau:

21 d

Calculation basis:

steady state

Conc. / dose:

0.002 mg/L

Temp.:

23.7 °C

pH:

7.1

Type:

BCF

Value:

< 10 dimensionless

Basis:

not specified

Time of plateau:

21 d

Calculation basis:

steady state

A bioconcentration study of the test item in carp, Cyprinus carpio, was conducted in compliance with the Test Methods Concerning a New Chemical Substance (Kashinhou). Based on a 96hr-LC50 of the test substance for zebra-fish (Danio rerio) (1.95 mg/L), two concentration levels were selected: 0.02 mg/L as a high exposure level and 0.002 mg/L as a low exposure level.

The test substance is a reaction product consisting of the main component (composition ratio 93.0%) and its isomer (1.1%), and three minor components (less than 1% for each component).

As the results of this study, the residual concentrations of p,p-isomer and m,p-isomer in fish were the detectable Umit or less, and the exposure period therefore was terminated after 28 days of exposure when the steady-states of these components were assumed to have been reached.

The bioconcentration factors (BCFs) of p,p-isomer and m,p-isomer in fish were 1 fold or less and 2 folds or less, respectively, at the high exposure level (0.02 mg/L group) and 10 folds or less and 20 folds or less, respectively, at the low exposure level (0.002 mg/L group).

Validity criteria fulfilled:

yes

Conclusions:

The test substance does not accumulate in organisms.

Executive summary:

In this study conducted to generally accepted scientific standards the test material (EC 444-860-9) was determined to not not bioaccumulate in organisms.

Description of key information

 The test substance does not accumulate in organisms.  The result of a bioaccumulation study with Cyprinus carpio following the procedures of the OECD guideline 305C showed a BCF of <10 for a test concentration of 0.002 mg/L (Institute of Ecotoxicology Co., 2005). Even though DMSO was used as solubilizer, the study is regarded as valid since both test concentrations (0.02 and 0.002 mg/L) are well below the water solubility of 9.9 mg/L of the test substance. 

Additional information

Study conducted to generally recognised scientific standards with GLP.