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EC number: 202-767-9 | CAS number: 99-57-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from publication.
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- acute toxicity
- Author:
- Lloyd et al.
- Year:
- 1 977
- Bibliographic source:
- Food and Cosmetics Toxicology
- Reference Type:
- publication
- Title:
- IARC MONOGRAPHS
- Author:
- IARC
- Year:
- 1 993
- Bibliographic source:
- IARC
- Reference Type:
- review article or handbook
- Title:
- Acute oral toxicity of test chemical
- Author:
- Richard J. Lewis
- Year:
- 2 012
- Bibliographic source:
- Sax's Dangerous Properties of Industrial Materials
- Reference Type:
- review article or handbook
- Title:
- Acute oral toxicity - Test chemical in rodent
- Author:
- S D Gangolli
- Year:
- 2 007
- Bibliographic source:
- The Dictionary of Substances and their Effects (DOSE)
- Reference Type:
- other: authoritative database
- Title:
- Acute oral toxicity by using test chemical
- Author:
- U.S. National Library of Medicine
- Year:
- 2 018
- Bibliographic source:
- ChemIDplus
- Reference Type:
- publication
- Title:
- Acute oral toxicity of test chemical
- Author:
- IFA GESTIS
- Year:
- 2 018
- Bibliographic source:
- IFA GESTIS
- Reference Type:
- other: authoritative database
- Title:
- Acute Toxicities of the given test chemical
- Author:
- U.S. National Library of Medicine
- Year:
- 2 008
- Bibliographic source:
- HSDB
- Reference Type:
- secondary source
- Title:
- HUMAN HEALTH TIER II ASSESSMENT
- Author:
- NICNAS
- Year:
- 2 018
- Bibliographic source:
- INVENTORY MULTITIERED ASSESSMENT AND PRIORITISATION (IMAP) NICNAS
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: As mentioned below
- Principles of method if other than guideline:
- Acute oral toxicity study of the given test chemical in rat.
- GLP compliance:
- not specified
- Test type:
- other: not specified
- Limit test:
- no
Test material
- Reference substance name:
- 2-amino-4-nitrophenol
- EC Number:
- 202-767-9
- EC Name:
- 2-amino-4-nitrophenol
- Cas Number:
- 99-57-0
- Molecular formula:
- C6H6N2O3
- IUPAC Name:
- 2-amino-4-nitrophenol
- Details on test material:
- - IUPAC Name: 2-amino-4-nitrophenol
- InChI: 1S/C6H6N2O3/c7-5-3-4(8(10)11)1-2-6(5)9/h1-3,9H,7H2
- Smiles: Nc1cc(ccc1O)[N+](=O)[O-]
- Molecular formula :C6H6N2O3
- Molecular weight :154.1244 g/mol
- Substance type:organic
- Physical state:Solid, powder yellow
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: CFY strain
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: weight range 90-122 g
- Fasting period before study: The animals were starved overnight before treatment.
Administration / exposure
- Route of administration:
- oral: unspecified
- Vehicle:
- other: Aqueous solution or suspension in 0.5% aqueous gum tragacanth
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 40%
- DOSAGE PREPARATION (if unusual): The test material was prepared as aqueous solution or suspension in 0.5% aqueous gum tragacanth containing 0.05% Na2SO3. - Doses:
- Range of 2000 - 3000 mg/kg bw
- No. of animals per sex per dose:
- five males and five females per groups of rats
- Control animals:
- yes
- Remarks:
- Rats treated with the vehicle alone served as controls
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: During the observation period of 14 days, a record was kept of all deaths and signs of toxicity.
- Necropsy of survivors performed: yes, all rats that died were examined macroscopically in an attempt to identify the target organs, and animals surviving to the end of the experiment were similarly examined at that time to detect any possible residual damage. There was no microscopic examination of the various tissues and organ systems. - Statistics:
- The LD50 and its 95% confidence limits were calculated from the mortality data either by the method of Litchfield & Wilcoxon (1949) or by that of Weil (1952).
Results and discussion
- Preliminary study:
- In a preliminary range finding study, the freshly prepared solutions or suspensions were administered to groups of two male and two female rats by oral intubation in a range of dosage volumes, in order to find the approximate median lethal oral dose (LD50).
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 2 400 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- 2 000 - 3 000
- Mortality:
- 50% mortality was observed at 2400 mg/kg bw in treated animals.
- Clinical signs:
- Signs of reaction to treatment, observed shortly after dosing with the compound, included lethargy and piloerection. Other reactions included increased salivation, ataxia, fine body tremors, changes in respiratory rate, dieresis and diarrhea. Also, produced orange-stained urine. These signs were not considered to be indicative of any specific target-organ toxicity.
- Body weight:
- not specified
- Gross pathology:
- Autopsy of the animals that died as a result of treatment revealed changes which, in many cases, included darkening of the liver and kidneys, darkening or pallor of the spleen, hemorrhage of the lungs and intestines, and injection of the intestinal and mesenteric blood vessels. Also, the rats showed orange staining of the peritoneal wall.
Autopsy of the survivors at the end of each experiment did not reveal any abnormalities indicative of residual systemic effects. - Other findings:
- not specified
Applicant's summary and conclusion
- Interpretation of results:
- other: Not classified
- Conclusions:
- The acute oral LD50 value was considered to be 2400 mg/kg bw, with 95% confidence limit of 2000 - 3000 mg/kg bw, when groups of rats (five males and five females) were treated with the given test chemical via oral route.
- Executive summary:
Acute oral toxicity study of the given test chemical was conducted in groups of rats (five males and five females) at the dose concentration range of 2000 - 3000 mg/kg bw.
The given test chemical was prepared as aqueous solution or suspension in 0.5% aqueous gum tragacanth, containing 0.05% Na2SO3 and administered via oral route. Rats treated with the vehicle alone served as control.
In a preliminary range finding study, the freshly prepared solutions or suspensions were administered to groups of two male and two female rats by oral intubation in a range of dosage volumes, in order to find the approximate median lethal oral dose (LD50).
After these preliminary range-finding tests had given a rough approximation of the LD50, larger groups of rats (five males and five females) were used in order to locate the median lethal dose more precisely. A logarithmic dosage interval of 1.6 was used for material.
During the observation period of 14 days, a record was kept of all deaths and signs of toxicity. Necropsy of survivors performed. All rats that died were examined macroscopically in an attempt to identify the target organs, and animals surviving to the end of the experiment were similarly examined at that time to detect any possible residual damage. There was no microscopic examination of the various tissues and organ systems.
The LD50 and its 95% confidence limits were calculated from the mortality data either by the method of Litchfield & Wilcoxon (1949) or by that of Weil (1952).
50% mortality was observed at 2400 mg/kg bw in treated animals. Signs of reaction to treatment, observed shortly after dosing with the compound, included lethargy and piloerection. Other reactions included increased salivation, ataxia, fine body tremors, and changes in respiratory rate, dieresis and diarrhea. Also, produced orange-stained urine. These signs were not considered to be indicative of any specific target-organ toxicity.
Autopsy of the animals that died as a result of treatment revealed changes which, in many cases, included darkening of the liver and kidneys, darkening or pallor of the spleen, hemorrhage of the lungs and intestines, and injection of the intestinal and mesenteric blood vessels. Also, the rats showed orange staining of the peritoneal wall. Autopsy of the survivors at the end of each experiment did not reveal any abnormalities indicative of residual systemic effects.
Under the condition of the study, the LD50 value was considered to be 2400 mg/kg bw, with 95% confidence limit of 2000 - 3000 mg/kg bw, when groups of rats (five males and five females) were treated with the given test chemical via oral route.
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